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Article Open Access

miRNA‑384‑5p regulates the progression of Parkinson's disease by targeting SIRT1 in mice and SH‑SY5Y cell

  • Authors:
    • Hongli Tao
    • Yan Liu
    • Yingjuan Hou
  • View Affiliations / Copyright

    Affiliations: Department of Psychiatry, Xianyang Central Hospital, Xianyang, Shaanxi 712000, P.R. China, Department of Neurology, Weinan Central Hospital, Weinan, Shaanxi 714000, P.R. China, Department of Neurology, The First Hospital of Yulin, Yulin, Shaanxi 718000, P.R. China
    Copyright: © Tao et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 441-450
    |
    Published online on: December 12, 2019
       https://doi.org/10.3892/ijmm.2019.4426
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Abstract

Parkinson's disease (PD) is the second most common neurodegenerative disorder. miR‑384‑5p expression has been shown to be increased in an in vitro model of PD; however, it remains unknown whether there are other molecules that can be regulated by miR‑384‑5p in in vivo and in vitro models of PD; thus, the present study aimed to elucidate this matter. Rotenone was applied for the establishment of in vitro and in vivo models of PD in the present study. Motor disability and equilibrium were determined by a swimming test and traction test, respectively. mRNA and protein levels were detected by reverse transcription‑quantitative polymerase chain reaction (RT‑qPCR) and western blot analysis, respectively. The association between miR‑384‑5p and Sirtuin 1 (SIRT1) expression was verified by dual luciferase reporter assay. The α‑synuclein aggregation was evaluated by immunofluorescence. The results from the in vitro model of PD demonstrated that, the mice in the PD group exhibited decreased scores in the swimming test and traction test, which were accompanied by increased α‑synuclein aggregation. In addition, the expression of miR‑384‑5p, which targeted the 3'untranslated region (3'UTR) of SIRT1, was verified to be increased in mice and SH‑SY5Y cells in the PD group, whereas SIRT1 exhibited the opposite changes. Moreover, increased mRNA and protein levels of p53 and FOXO1 were observed in mice and SH‑SY5Y cells in the PD group. In addition, the SH‑SY5Y cells in the PD group exhibited a higher cell apoptotic rate. On the whole, the findings of this study demonstrate that miRNA‑384‑5p promotes the progression of PD by targeting SIRT1.
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View References

1 

de Rijk MC, Launer LJ, Berger K, Breteler MM, Dartigues JF, Baldereschi M, Fratiglioni L, Lobo A, Martinez-Lage J, Trenkwalder C and Hofman A: Prevalence of Parkinson's disease in Europe: A collaborative study of population-based cohorts. Neurologic Diseases in the Elderly Research Group. Neurology. 54:S21–S23. 2000.PubMed/NCBI

2 

de Lau LM and Breteler MM: Epidemiology of Parkinson's disease. Lancet Neurol. 5:525–535. 2006. View Article : Google Scholar : PubMed/NCBI

3 

Dickson DW, Braak H, Duda JE, Duyckaerts C, Gasser T, Halliday GM, Hardy J, Leverenz JB, Del Tredici K, Wszolek ZK and Litvan I: Neuropathological assessment of Parkinson's disease: Refining the diagnostic criteria. Lancet Neurol. 8:1150–1157. 2009. View Article : Google Scholar : PubMed/NCBI

4 

Jankovic J: Parkinson's disease: Clinical features and diagnosis. J Neurol Neurosurg Psychiatry. 79:368–376. 2008. View Article : Google Scholar : PubMed/NCBI

5 

Seidl SE, Santiago JA, Bilyk H and Potashkin JA: The emerging role of nutrition in Parkinson's disease. Front Aging Neurosci. 6:362014. View Article : Google Scholar : PubMed/NCBI

6 

White AR, Kanninen KM and Crouch PJ: Metals and neurode-generation: Restoring the balance. Front Aging Neurosci. 7:1272015. View Article : Google Scholar

7 

Allen MT and Levy LS: Parkinson's disease and pesticide exposure-a new assessment. Crit Rev Toxicol. 43:515–534. 2013. View Article : Google Scholar : PubMed/NCBI

8 

Hutvágner G and Zamore PD: A microRNA in a multiple-turnover RNAi enzyme complex. Science. 297:2056–2060. 2002. View Article : Google Scholar : PubMed/NCBI

9 

Zeng Y and Cullen BR: Sequence requirements for micro RNA processing and function in human cells. RNA. 9:112–123. 2003. View Article : Google Scholar : PubMed/NCBI

10 

Wienholds E and Plasterk RH: MicroRNA function in animal development. FEBS Lett. 579:5911–5922. 2005. View Article : Google Scholar : PubMed/NCBI

11 

Thome AD, Harms AS, Volpicelli-Daley LA and Standaert DG: MicroRNA-155 regulates alpha-synuclein-induced infammatory responses in models of Parkinson disease. J Neurosci. 36:2383–2390. 2016. View Article : Google Scholar : PubMed/NCBI

12 

Caggiu E, Paulus K, Mameli G, Arru G, Sechi GP and Sechi LA: Differential expression of miRNA 155 and miRNA 146a in Parkinson's disease patients. eNeurologicalSci. 13:1–4. 2018. View Article : Google Scholar : PubMed/NCBI

13 

Zhang X, Yang R, Hu BL, Lu P, Zhou LL, He ZY, Wu HM and Zhu JH: Reduced circulating levels of miR-433 and miR-133b are potential biomarkers for Parkinson's disease. Front Cell Neurosci. 11:1702017. View Article : Google Scholar : PubMed/NCBI

14 

Ge H, Yan Z, Zhu H and Zhao H: MiR-410 exerts neuroprotective effects in a cellular model of Parkinson's disease induced by 6-hydroxydopamine via inhibiting the PTEN/AKT/mTOR signaling pathway. Exp Mol Pathol. 109:16–24. 2019. View Article : Google Scholar : PubMed/NCBI

15 

Jiang M, Yun Q, Shi F, Niu G, Gao Y, Xie S and Yu S: Downregulation of miR-384-5p attenuates rotenone-induced neurotoxicity in dopaminergic SH-SY5Y cells through inhibiting endoplasmic reticulum stress. Am J Physiol Cell Physiol. 310:C755–C763. 2016. View Article : Google Scholar : PubMed/NCBI

16 

Inden M, Kitamura Y, Abe M, Tamaki A, Takata K and Taniguchi T: Parkinsonian rotenone mouse model: Reevaluation of long-term administration of rotenone in C57BL/6 mice. Biol Pharm Bull. 34:92–96. 2011. View Article : Google Scholar : PubMed/NCBI

17 

Haobam R, Sindhu KM, Chandra G and Mohanakumar KP: Swim-test as a function of motor impairment in MPTP model of Parkinson's disease: A comparative study in two mouse strains. Behav Brain Res. 163:159–167. 2005. View Article : Google Scholar : PubMed/NCBI

18 

Dai SF, Han G, Li Y, Yu D, Zhang D, Feng Y, Zhao JI and Sun Y: Effects of nicotine on the microglia of Parkinson's disease mice. Asian J Pharmacodyn Pharmacokin. 8:319–323. 2008.

19 

Livak KJ and Schmittgen TD: Analysis of relative gene expression data using real-time quantitative PCR and the 2(-Delta Delta C(T)) method. Methods. 25:402–408. 2001. View Article : Google Scholar

20 

Lin TK, Chen SD, Chuang YC, Lin HY, Huang CR, Chuang JH, Wang PW, Huang ST, Tiao MM, Chen JB and Liou CW: Resveratrol partially prevents rotenone-induced neurotoxicity in dopaminergic SH-SY5Y cells through induction of heme oxygenase-1 dependent autophagy. Int J Mol Sci. 15:1625–1646. 2014. View Article : Google Scholar : PubMed/NCBI

21 

Salimian N, Peymani M, Ghaedi K and Nasr Esfahani MH: Modulation in miR-200a/SIRT1axis is associated with apoptosis in MPP+-induced SH-SY5Y cells. Gene. 674:25–30. 2018. View Article : Google Scholar : PubMed/NCBI

22 

Singh P, Hanson PS and Morris CM: SIRT1 ameliorates oxida-tive stress induced neural cell death and is down-regulated in Parkinson's disease. BMC Neurosci. 18:462017. View Article : Google Scholar

23 

Zhou Y, Wang S, Li Y, Yu S and Zhao Y: SIRT1/PGC-1α signaling promotes mitochondrial functional recovery and reduces apoptosis after intracerebral hemorrhage in rats. Front Mol Neurosci. 10:4432018. View Article : Google Scholar

24 

Wang CY, Sun ZN, Wang MX and Zhang C: SIRT1 mediates salidroside-elicited protective effects against MPP+ -induced apoptosis and oxidative stress in SH-SY5Y cells: involvement in suppressing MAPK pathways. Cell Biol Int. 42:84–94. 2018. View Article : Google Scholar

25 

Wang Z, Sun L, Jia K, Wang H and Wang X: miR-9-5p modulates the progression of Parkinson's disease by targeting SIRT1. Neurosci Lett. 701:226–233. 2019. View Article : Google Scholar : PubMed/NCBI

26 

Lee JT and Gu W: SIRT1: Regulator of p53 deacetylation. Genes Cancer. 4:112–117. 2013. View Article : Google Scholar : PubMed/NCBI

27 

Yao H, Yao Z, Zhang S, Zhang W and Zhou W: Upregulation of SIRT1 inhibits H2O2-induced osteoblast apoptosis via FoxO1/β-catenin pathway. Mol Med Rep. 17:6681–6690. 2018.PubMed/NCBI

28 

Hori YS, Kuno A, Hosoda R and Horio Y: Regulation of FOXOs and p53 by SIRT1 modulators under oxidative stress. PLoS One. 8:e738752013. View Article : Google Scholar : PubMed/NCBI

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Copy and paste a formatted citation
Spandidos Publications style
Tao H, Liu Y and Hou Y: miRNA‑384‑5p regulates the progression of Parkinson's disease by targeting SIRT1 in mice and SH‑SY5Y cell. Int J Mol Med 45: 441-450, 2020.
APA
Tao, H., Liu, Y., & Hou, Y. (2020). miRNA‑384‑5p regulates the progression of Parkinson's disease by targeting SIRT1 in mice and SH‑SY5Y cell. International Journal of Molecular Medicine, 45, 441-450. https://doi.org/10.3892/ijmm.2019.4426
MLA
Tao, H., Liu, Y., Hou, Y."miRNA‑384‑5p regulates the progression of Parkinson's disease by targeting SIRT1 in mice and SH‑SY5Y cell". International Journal of Molecular Medicine 45.2 (2020): 441-450.
Chicago
Tao, H., Liu, Y., Hou, Y."miRNA‑384‑5p regulates the progression of Parkinson's disease by targeting SIRT1 in mice and SH‑SY5Y cell". International Journal of Molecular Medicine 45, no. 2 (2020): 441-450. https://doi.org/10.3892/ijmm.2019.4426
Copy and paste a formatted citation
x
Spandidos Publications style
Tao H, Liu Y and Hou Y: miRNA‑384‑5p regulates the progression of Parkinson's disease by targeting SIRT1 in mice and SH‑SY5Y cell. Int J Mol Med 45: 441-450, 2020.
APA
Tao, H., Liu, Y., & Hou, Y. (2020). miRNA‑384‑5p regulates the progression of Parkinson's disease by targeting SIRT1 in mice and SH‑SY5Y cell. International Journal of Molecular Medicine, 45, 441-450. https://doi.org/10.3892/ijmm.2019.4426
MLA
Tao, H., Liu, Y., Hou, Y."miRNA‑384‑5p regulates the progression of Parkinson's disease by targeting SIRT1 in mice and SH‑SY5Y cell". International Journal of Molecular Medicine 45.2 (2020): 441-450.
Chicago
Tao, H., Liu, Y., Hou, Y."miRNA‑384‑5p regulates the progression of Parkinson's disease by targeting SIRT1 in mice and SH‑SY5Y cell". International Journal of Molecular Medicine 45, no. 2 (2020): 441-450. https://doi.org/10.3892/ijmm.2019.4426
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