Open Access

The role of myoglobin in epithelial cancers: Insights from transcriptomics

  • Authors:
    • Anne Bicker
    • Theresa Nauth
    • Daniela Gerst
    • Mostafa Ahmed Aboouf
    • Joachim Fandrey
    • Glen Kristiansen
    • Thomas Alexander Gorr
    • Thomas Hankeln
  • View Affiliations

  • Published online on: December 18, 2019     https://doi.org/10.3892/ijmm.2019.4433
  • Pages: 385-400
  • Copyright: © Bicker et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

The muscle‑associated respiratory protein myoglobin (MB) is expressed in multiple types of cancer, including breast and prostate tumors. In Kaplan‑Meier analyses of the two tumor types, MB positivity is associated with favorable prognoses. Despite its well‑characterized function in myocytes, the role of MB in cancer remains unclear. To study the impact of endogenous MB expression, small interfering RNA MB‑knockdown cells were engineered using breast, prostate and colon cancer cell lines (MDA‑MB468, LNCaP, DLD‑1), and their transcriptomes were investigated using RNA‑Seq at different oxygen levels. In MB‑positive cells, increased expression of glycolytic genes was observed, which was possibly mediated by a higher activity of hypoxia‑inducible factor 1α. In addition, the results of the gene set enrichment analysis suggested that MB contributed to fatty acid transport and turnover. MB‑positive, wild‑type‑p53 LNCaP cells also exhibited increased expression of p53 target genes involved in cell cycle checkpoint control and prevention of cell migration. MB‑positive cells expressing mutant p53 exhibited upregulation of genes associated with prolonged cancer cell viability and motility. Therefore, it was hypothesized that these transcriptomic differences may result from MB‑mediated generation of nitric oxide or reactive oxygen species, thus employing established enzymatic activities of the globin. In summary, the transcriptome comparisons identified potential molecular functions of MB in carcinogenesis by highlighting the interaction of MB with key metabolic and regulatory processes.

Introduction

The red color of the vertebrate skeletal and cardiac muscles originates from a high expression level of the respiratory protein myoglobin (MB) (1,2). The globin fold of MB harbors an iron-containing heme group that allows the binding of gaseous ligands such as O2 and nitric oxide (NO·) (3,4). The impact of MB as an intracellular O2 storage and transport molecule was indirectly evidenced when the viability of Mb−/− mice required compensatory adaptions in cardiac and striated muscles, including increased vessel densities, higher blood viscosity and elevated expression of the hypoxia-inducible transcription factors hypoxia-inducible factor 1α (HIF1α) and HIF2α (5-7). An additional function of oxygenated MB (oxy-MB) in aerobic muscles is to convert harmful NO· to inert nitrate by enzymatic dioxygenase activity (4,8). Mb−/− mice exhibited a greater sensitivity for altered NO· concentrations in cardiomyocytes compared with respective wild-type animals and were more susceptible to NO·-induced changes in the vascular tone, cardiac functions and energetic parameters (8). Oxy-MB was further reported to scavenge toxic reactive oxygen species (ROS) for cell protection in C2C12 mouse myotubes and in the brain of the common carp (9-11). Under hypoxia, deoxygenated MB (deoxy-MB) can produce NO·, as observed in studies on mouse smooth muscle cells in vivo; this regulates NO·-mediated vasodilation of adjacent blood vessels in isolated hearts of Mb+/+ vs. Mb−/− mice, thus reducing ischemia-induced cardiac damage (12). MB harbors various fatty acid binding sites with high affinity for palmitic and oleic acid (13-15). Thus, oxy-MB has been proposed to bind and transport fatty acids (FAs) and acylcarnitines to mitochondria in an O2-dependent manner (14,15). In addition, since the cardiomyocytes of Mb−/− mice were less able to oxidize fatty acids, older mice developed a lipid overload phenotype that impaired the heart function (16,17). These results suggested that oxy-MB may serve a non-canonical role by promoting FA turnover in vivo.

Ectopic MB expression at a level of 1:100-1:1,000 compared to striated muscle was further reported in several tumor types, including breast, prostate, non-small lung and colon cancer, renal cell carcinoma, head and neck squamous cell carcinoma, osteosarcoma and leukemic bone marrow (18-25). Instead of using the myocytic standard gene promoter, MB is transcribed from an alternative upstream promoter region in cancer cells, which can be specifically induced by hypoxia and silenced by hormonal treatments (26,27). In addition, MB staining was enhanced at hypoxic, perinecrotic central areas in avascular, non-invasive ductal carcinoma in situ (DCIS) breast tumors (28). Compared to the low-level expression of MB in the healthy breast epithelium, MB production in mammary malignancies increases up to 350-fold (29). Overall, MB positivity was detected in ~40% of primary breast tumors, mainly in a mosaic-like pattern in luminal-type, estrogen receptor (ER)-positive cases (21), and in ~53% of prostate cancer tumors, mostly in androgen-receptor positive and poorly differentiated cases (24). Kaplan-Meier survival analyses of a large cohort of patients with mammary carcinoma associated high MB expression with beneficial prognostic outcomes for cases with positive or negative ERα receptor status (21). Additionally, a trend towards prolonged recurrence-free patient survival was observed for MB-positive compared with -negative tumors in a cohort of poorly differentiated prostate tumors (24). In contrast to a hypothetical tumor-suppressing role of MB in these tumor entities, patients with lung adenocarcinoma with high MB levels in tumor biopsies exhibited poor prognostic outcomes (22). This discrepancy indicates potential tumor type-specific differences for the role of MB in cancer cells.

Despite a limited number of initial experiments, no in-depth characterization of the molecular role of MB endogenously expressed in tumor cells has been achieved. As breast, prostate and colon cancer exhibit several pathological and biochemical commonalities, and in order to assess a broader spectrum of potential molecular functions of MB in epithelial cancers, the present study aimed to determine the impact of endogenous MB expression in three different cancer cell lines representing the above malignancies: MDA-MB468, LNCaP and DLD-1. To keep this approach free of a priori hypotheses, transcriptome-wide cDNA sequencing (RNA-Seq) of MB-expressing (MB+) cancer cell lines and respective RNA interference (RNAi)-mediated MB-knockdown cells (siMB) was conducted. The bioinformatic identification of differentially expressed gene categories and pathways may provide valuable information about the molecular role of MB in tumor cells.

Materials and methods

Cell culture

MDA-MB468 and DLD-1 cells were cultured in DMEM/HamsF12 with L-glutamine (PAA Laboratories; GE Healthcare Bio-Sciences Austria GmbH); LNCaP cells were cultured in RPMI-1640 medium with L-glutamine (Invitrogen; Thermo Fisher Scientific, Inc.). All cells were supplemented with 10% Fetal Bovine Serum Gold (PAA Laboratories; GE Healthcare Bio-Sciences Austria GmbH). Hera cell 240 (MultiTemp Scientific AG), IG150 (Jouan; Thermo Fisher Scientific, Inc.) and CB 53 (Binder GmbH) incubators were used for cell culture. For normoxic conditions, cells were cultured in room air with 5% CO2, whereas hypoxic incubation was conducted with 1% O2 and 5% CO2; both at 37°C in H2O-saturated atmosphere. MDA-MB468, DLD-1 and LNCaP cells were authenticated by SNP typing (Multiplexion GmbH) and approved for mycoplasma negativity using the VenorGeM Mycoplasma PCR detection kit (Minerva Biolabs GmbH) prior to experiments. Transient MB-knockdown cells for all three cell lines were generated using small interfering (si)RNA. Cells at 50-60% confluency were seeded in 24-well plates. After 24 h of incubation, 20 pmol MB siRNA (HS_MB_6, 2607575; anti-sense sequence, 5′-UGA UUA AUC AGA CAA UUG CTA-3′; Qiagen GmbH) or scrambled (scr) RNA (cat. no. 1022076; Qiagen GmbH) were diluted in Opti-MEM (cat. no. 31985062; Thermo Fisher Scientific, Inc.) for each well. Lipofectamine® 2000 (1 µl; Thermo Fisher Scientific, Inc.) was added to 50 µl Opti-MEM and incubated for 5 min at room temperature. The two prepared solutions were then gently mixed together and incubated for 20 min at room temperature. The oligomer-Lipofectamine 2000 complexes were then added to the cells. After 24 h of incubation, the culture medium was changed.

RNA-Seq experiments

Following 72 h of incubation under 1% O2 or normoxia, RNA was extracted from cells using the RNeasy Mini kit (Qiagen GmbH), including the RNase-Free DNase I treatment (Qiagen GmbH) for DNA digestion. RNA integrity was confirmed using the Agilent RNA 6000 Nano kit on a 2100 Bioanalyzer (Agilent Technologies GmbH). RNA samples from four replicate cell passages were pooled for each condition and cell line. Illumina TruSeq Total RNA transcriptome libraries (Illumina, Inc.) were generated for the pools of the siMB cell types (3 cell lines and 2 O2 conditions) and the respective MB+ controls (StarSEQ GmbH). The 50 bp single-end Illumina sequencing was performed by the next generation sequencing core facility of the Biology Department of Johannes Gutenberg University (Mainz, Germany) on an Illumina HiSeq2500 sequencing platform.

Data processing

Using the CLC Genomics Workbench 8.0.1 sequence trimmer (Qiagen GmbH), 12 terminal nucleotides from the 5′ end, remaining Illumina adapters and low-quality sequences (below Phred 13) were removed from each read. The minimum sequence length was set to 15 bp, allowing ≤2 ambiguous nucleotides per read. The reads were then mapped to the annotated human genome hg19 (ftp.ensembl.org/pub/release-75/gtf/homo_sapiens/Homo_sapiens.GRCh37.75. gtf.gz) with the CLC Genomics Workbench 8.0.1 RNA-Seq tool. Mapping parameters were set as default, but included intergenic regions and allowed ≤10 hits per read. Paired two-group comparisons were conducted between the mapping results of siMB and MB+ cells for the three cell lines with or without hypoxia using the CLC Genomics Workbench 8.0.1 with the original reads per kilobase of transcript per million mapped reads (RPKM) values (30). Proportion-based statistics were calculated with Kal's Z-tests [Bonferroni- and false discovery rate (FDR)-corrected] to identify statistically significant differentially expressed genes at [−log10(p)]≥0.5 (31).

Variant detection

Low frequency variant detection was performed with the CLC Genomics Workbench 8.5.1 at default parameters using a minimum read count of 6, a minimum coverage of six reads and including a read direction filter of 1%. Only variants of QUAL ≥100 (probability that a particular variant exists ≥0.9999999999) were listed and annotated with human genome hg19 gene track overlap information to infer potential non-synonymous amino acid changes.

Interpretation of RNA-Seq data

To access the transcriptome-wide effects of MB expression in epithelial cancer cells, all genes that were differentially expressed between siMB and MB+ cells were identified (Table I). A list of genes that were differentially expressed depending on the MB status in at least four of the six gene lists was compiled to filter general MB functions common to the majority of the investigated cell types. This merged gene list was used for gene set enrichment analysis (hypergeometric tests; FDR-corrected P<0.05) using the Cytoscape 3 application BiNGO 3.0.3 (32) to generate a map of significantly overrepresented Gene Ontology (GO)-categories.

Table I

Specifications of RNA-Seq datasets generated in this study.

Table I

Specifications of RNA-Seq datasets generated in this study.

DatasetTotal mapped readsDifferentially expressed genesDownregulation of MBHypoxia induction
LDHAGLUT1VEGFA
siMB MB468 Hx
MB+ MB468 Hx
28,841,086
23,674,893
807 (224 up, 583 down)−37.9-foldbMB: 1.3a; MB+: 1.4bsiMB: 1.2; MB+: 1.3siMB: 1.8; MB+: 1.2
siMB MB468 Nx
MB+ MB468 Nx
46,544,990
36,990,522
683 (241 up, 442 down)−26.1-foldb
siMB DLD-1 Hx
MB+ DLD-1 Hx
28,156,099
26,890,772
549 (276 up, 273 down)−7.7-foldasiMB: 3.1b; MB+: 2.8bsiMB: 2.4b; MB+: 2.1bsiMB: 4.4b; MB+: 5.0b
siMB DLD-1 Nx
MB+ DLD-1 Nx
53,342,155
47,002,503
160 (77 up, 83 down)−5.9-folda
siMB LNCaP Hx
MB+ LNCaP Hx
47,393,215
32,967,690
857 (371 up, 486 down)−15.9-foldbsiMB: 2.5b; MB+: 1.7bsiMB: 1.7b; MB+: 1.5bsiMB: 4.1b; MB+: 5.5b
siMB LNCaP Nx
MB+ LNCaP Nx
50,920,424
48,109,894
569 (284 up, 285 down)−11.1-foldb

a P≤0.1;

b P≤0.01. MB, myoglobin; siMB, cells transfected with small interfering RNA targeting MB; MB+ cells transfected with scrambled small interfering RNA; Hx, hypoxia; Nx, normoxia.

For a more detailed analysis of the impact of MB in each cell line under each O2 condition, the six lists of genes differentially expressed between siMB and MB+ cells in matching conditions were further analyzed for GO term enrichment (P<0.05) and gene enrichment in KEGG and Biocarta pathways using the DAVID Bioinformatics Resources 6.7 functional annotation tool (33,34), choosing either the upregulated or downregulated genes as inputs. Genes differentially expressed between siMB and MB+ cells were further annotated and interpreted by Core Ingenuity Pathway analysis (IPA) (http://www.ingenuity.com). Analyses were conducted using the fold-changes determined by CLC two-group-comparisons, including the IPA a priori knowledge of direct and indirect relationships between genes observed in all human tissues. For visualization, a list of significantly active upstream regulators in each condition was compiled based on the direction of regulation of their target genes.

Results

RNA-Seq data generation

To investigate the function of endogenously expressed MB in epithelial cancer cells, siRNA was used to generate MB-knockdown breast (MDA-MB468), prostate (LNCaP) and colon (DLD-1) cancer cells, and their transcriptome profiles were compared to those of matching MB-wild-type cells treated with scr siRNA as a control. Different tumor types were selected for studying MB expression to discriminate tumor-specific effects [e.g., ER status (27)] from common changes that may be associated with MB expression throughout different tumor types of epithelial origin. As MB can be either oxygenated or deoxygenated, experiments for all three cell lines were conducted in room air (normoxia) and 1% O2 (hypoxia), the latter causing a fractional MB O2 desaturation of ~42% (35). To specifically study the impact of MB in cells adapted to long-term hypoxia, mimicking tumors, the cells were incubated for 72 h at hypoxic vs. normoxic conditions; previous experiments on MDA-MB468 siRNA MB-knockdown cells demonstrated strong phenotypic effects at 72 h (28). Using Illumina transcriptome sequencing and read mapping to the annotated human genome, gene expression profiles were generated for each cell line and condition. The numbers of successfully mapped reads ranged between 23.7 and 53.3 Mio (Table I).

Functional annotation of differentially regulated genes

Following RNAi, MB was downregulated 6- and 8-fold in DLD-1, 11- and 16-fold in LNCaP and 26- and 38-fold in MDA-MB468 cells compared with the control cells in normoxic and hypoxic conditions, respectively. In addition, between 160 and 857 genes were differentially expressed between siMB and MB+ control cells under equivalent oxygen conditions (Tables I and SI). This divergence implied differences in the impact of MB on specific epithelial cell lines and encouraged the investigation of the generic and cell-type specific effects of MB expression in epithelial cancer cells. Differentially expressed genes were subjected to GO term analysis, and overrepresentations of GO terms within gene lists were statistically evaluated.

The effects of MB expression common among the three epithelial cancer cell lines were summarized by BiNGO analysis of a subset of 145 genes, which were consistently differentially expressed in at least four of the six experimental groups (Table I). A number of overrepresented GO terms, presented as colored nodes in Fig. 1, were associated with metabolic shifts (i.e., 'glycolysis', 'fatty acid biosynthetic process', 'oxidative phosphorylation' and 'generation of precursor metabolites and energy') and anti-apoptotic cell signaling (i.e., 'anti-apoptosis', 'negative regulation of programmed cell death' and 'regulation of DNA damage response', signaling transduction by p53 class mediator'). These terms were linked to the overrepresented nodes 'cell cycle' and 'protein ubiquiti-nation' in the BiNGO graph. In addition, overrepresentation of the GO categories 'response to hypoxia', 'response to oxidative stress' and 'response to ROS' was observed, suggesting that MB also participates in cellular response to oxidative stress.

The present study further aimed to identify individual cell type- and O2 level-specific characteristics in the three cancer cell lines (Table II). GO term and KEGG and Biocarta pathway enrichment analyses were performed separately for each subset of significantly upregulated and downregulated genes to compare siMB and MB+ cells (Table SI). Lists of enriched GO terms revealed a number of categories that repeatedly appeared across all studied cell types. These functional terms were related to those identified before in the global BiNGO analysis. In addition, the terms were linked to a range of known molecular functions exerted by MB. Several GO categories unique either to a certain cell type or to the O2 level were also identified (Table II).

Table II

Summary of overrepresented Biological Process GO categories.

Table II

Summary of overrepresented Biological Process GO categories.

A, GO terms of genes differentially expressed in siMB vs. MB+ MDA-MB468 cells

Hypoxia/ROS/NO·Metabolism Apoptosis/Migration/MotionOthers
Response to oxidative stress ↑↓H, ↑↓NGlycolysis ↓H, ↓NInduction of programmed cell death ↑↓H
Induction of apoptosis ↑↓H
Translation ↑↓H, ↑↓N
Chromatin assembly ↑H, ↑N
Response to hypoxia ↓H, ↓NLipid biosynthetic process ↑HNegative regulation of programmed cell death ↓H, ↓N
Regulation of programmed cell death ↓H, ↑↓N
Cytoskeleton organization ↑↓N
Regulation of cell proliferation ↓H, ↑↓N
Response to O2 levels ↓H, ↓NSteroid metabolic process ↑H, ↑NNegative regulation of apoptosis ↓H, ↓N
Regulation of apoptosis ↓H, ↑↓N
Positive regulation of cell proliferation ↓H, ↓N
Cell proliferation ↓N
Response to ROS ↓H, ↓N
Cholesterol metabolic process ↑N
Anti-apoptosis ↓H, ↓N
Apoptosis ↓H, ↓↑N
Negative regulation of cell proliferation ↑↓H, ↑N
Inflammatory response ↑H, ↑↓N
O2 reduction ↓H, ↑↓NSterol metabolic process ↑N
Unsaturated fatty acid metabolic process ↓N
Cell motion ↓H, ↓N
Cell migration ↓H, ↓N
Cell adhesion ↓H, ↓N
Mitotic cell cycle ↓H, ↓N
Cell cycle process ↓H, ↓N
Regulation of cell cycle ↓H, ↓N
N compound biosynthetic process ↓HUnsaturated fatty acid biosynthetic process ↓NPositive regulation of cell motion ↓H, ↓N
Positive regulation of cell migration ↓H, ↓N
Negative regulation of ubiquitin protein
ligase activity during mitotic cell cycle
↓H, ↓N
Regulation of NO· biosynthetic
process ↓H, ↓N
Generation of precursor metabolites & energy
↓H, ↓N
Negative regulation of cell motion ↓H, ↓N
Negative regulation of cell migration ↓N
Positive regulation of ubiquitin protein ligase activity during mitotic cell cycle ↓H, ↓N

B, GO terms of genes differentially expressed in siMB vs. MB+ LNCaP cells

Hypoxia/ROS/NO·Metabolism Apoptosis/Migration/MotionOthers

Response to oxidative stress ↓H, ↓NGlycolysis ↓H, ↓NInduction of programmed cell death ↓HTranslation ↓H, ↓N
Response to hypoxia ↓H, ↓NEicosanoid metabolic process ↑H
Unsaturated fatty acid metabolic process ↑H
Induction of apoptosis ↓H
Negative regulation of programmed cell death ↑↓H, ↓N
Chromatin assembly ↑H, ↑N
Cytoskeleton organization ↑H, ↑N
Response to O2 levels ↓H, ↓NUnsaturated fatty acid biosynthetic process ↑H
Fatty acid metabolic process ↑N
Apoptosis ↑H, ↑N
Cell motion ↑H, ↑N
Regulation of cell proliferation ↑H, ↑N
Positive regulation of cell proliferation
↓H, ↓N
Response to ROS ↓H, ↓NFatty acid biosynthetic process ↑H
Generation of precursor metabolites & energy ↓H, ↓N
Cell migration ↑H
Cell adhesion ↑H
Negative regulation of cell proliferation ↑H, ↑N
Mitotic cell cycle ↓H, ↓N
O2 reduction ↑↓H, ↑↓NOxidative phosphorylation ↓H, ↓N
Glycerophospholipid metabolic process ↑N
Negative regulation of cell motion ↑H
Negative regulation of cell migration ↑H
Cell cycle process ↓H, ↓N
Regulation of cell cycle ↓H, ↓N
Positive regulation of O2 & ROS
metabolic process ↑H
β-oxidation ↑NRegulation of programmed cell death ↑↓H, ↓N
Negative regulation of apoptosis ↑↓H, ↓N
Negative regulation of ubiquitin protein ligase activity during mitotic cell cycle ↓H, ↓N
N compound biosynthetic process ↓H, ↓NSterol biosynthetic process ↑NRegulation of apoptosis ↑↓H, ↓N
Anti-apoptosis ↑↓H, ↑↓N
Positive regulation of ubiquitin protein ligase activity during mitotic cell cycle ↓H, ↓N

C, GO terms of genes differentially expressed in siMB vs. MB+ DLD-1 cells

Hypoxia/ROS/NO·Metabolism Apoptosis/Migration/MotionOthers

Response to oxidative stress ↓H, ↓NGlycolysis ↑H
Cholesterol metabolic process ↓H
Induction of programmed cell death ↑↓H
Induction of apoptosis ↑↓H
Negative regulation of programmed cell death ↑↓H, ↓N
Translation ↑↓H, ↑↓N
Cell cycle process ↑H, ↑N
Cell division ↑H, ↑N
Response to ROS ↓H, ↓NSterol metabolic process ↓HRegulation of programmed cell death ↑↓H, ↓N
Negative regulation of apoptosis ↑↓H, ↓N
Regulation of cell cycle ↓H
Cell cycle arrest ↓H
O2 reduction ↓NCholesterol biosynthetic process ↓HRegulation of apoptosis ↑↓H, ↑↓N
Anti-apoptosis ↑↓H
Cytoskeleton organization ↓H
Regulation of cell proliferation ↓H
N compound biosynthetic process ↑H, ↓NSterol biosynthetic process ↓HApoptosis ↑↓H, ↓N
Cell motion ↓N
Cell proliferation ↑H, ↓N
Negative regulation of ubiquitin protein ligase activity during mitotic cell cycle ↑↓H
Positive regulation of N compound metabolic process ↓HGluconeogenesis ↑NPositive regulation of cell motion ↑H
Positive regulation of cell migration ↑H
Positive regulation of ubiquitin protein ligase activity during mitotic cell cycle ↑↓H, ↑N

[i] ↑, upregulated and ↓, downregulated genes in siMB vs. MB+ cells at each O2 condition. Terms printed in bold were also enriched in other cell lines or at other O2 levels. H, 1%O2; N, normoxia; siMB, cells transfected with small interfering RNA targeting myoglobin; MB+ cells transfected with scrambled small interfering RNA; ROS, reactive oxygen species; NO·, nitric oxide.

GO terms indicate a metabolic shift in siMB cells

In prostate and breast cancer cells, the GO categories 'response to O2 levels' and 'response to hypoxia' were overrepresented in the set of downregulated genes in siMB cells independent of the O2 conditions (Table II). Since these genes included a number of known hypoxia marker genes (Table SI), these results suggested that MB may modulate the cellular hypoxia response in breast and prostate cancer cell lines.

GO enrichment analysis further indicated a metabolic shift towards a reduced rate of glycolysis in MB-knockdown prostate and breast cancer cells under the two O2 conditions. This was evidenced by the downregulation of the hypoxia-regulated lactate dehydrogenase A (LDHA) gene and the glycolytic genes triosephospate isomerase 1 (TPI1), GAPDH, protein kinase CGMP-dependent 1 (PGK1) and glycerol-3-phosphate acyltransferase, mitochondrial (GPAM1). By contrast, MB-knockdown in hypoxic colon cancer cells resulted in an increase in glycolytic flux, as LDHA and the glycolysis genes TPI1, PGK1, GPAM1 and enolase 1 were upregulated (Tables SI and SII).

In normoxic MB-knockdown breast cancer cells, the GO categories 'unsaturated fatty acid metabolic process' and 'unsaturated fatty acid biosynthetic process' were overrepresented in the set of downregulated genes (Table II), including fatty acid desaturase 3 and δ4-desaturase, sphingolipid 1 (Tables SI and SIII). By contrast, genes associated with sterol, cholesterol and steroid metabolic processes were upregulated in MB-knockdown breast cancer cells, such as sterol regulatory element binding transcription factor 1 (SREBF1), phosphomevalonate kinase, 24-dehydrocholesterol reductase (DHCR24) and cytochrome P450 1B1 (Tables SI and SIII). These results suggested that oxy-MB may stimulate the metabolism of unsaturated fatty acids in oxygenated breast cancer cells. However, the globin may also interfere with the synthesis of sterols.

In prostate cancer cells, siMB treatment resulted in an increased ability to metabolize or synthetize fatty acids, sterols and eicosanoids, indicated by the upregulation of hydroxyacyl-CoA dehydrogenase trifunctional multienzyme complex β, DHCR24, sterol-C5-desaturase, 15-hydroxypros-taglandin dehydrogenase (HPGD), elongation of very long chain fatty acids protein 5 (ELOVL5) and acyl-CoA synthetase long chain family member 3 in normoxic siMB cells, and by upregulation of ELOVL5, HPGD, fatty acid synthase and phospholipase A2 group V in siMB hypoxic cells (Tables SI and SIII). By contrast, in hypoxic MB-knockdown colon cancer cells, the expression of genes associated with cholesterol and sterol metabolism and biosynthesis [DHCR7, 3-hydroxy-3-methylglutaryl-CoA synthase 1 (HMGCS1), farnesyl diphosphate synthase, farnesyl-diphosphate farne-syltransferase 1 and SREBF1] was decreased (Tables SI and SIII). In summary, the results of the transcriptome analysis suggested that MB may regulate fatty acid metabolism in a cell-type specific manner.

MB expression is associated with apoptosis and migration

The GO categories 'induction of programmed cell death' and 'induction of apoptosis' were overrepresented in the set of downregulated genes in hypoxic MB-knockdown prostate cancer cells, suggesting that MB positivity may be associated with the stimulation of apoptotic processes in these cells. One option of maintaining apoptosis is by triggering cell cycle checkpoint signaling (36). Accordingly, in both normoxic and hypoxic siMB LNCaP cells, the GO categories 'regulation of cell cycle' and 'positive regulation of cell proliferation' were overrepresented among the downregulated genes, whereas the category 'negative regulation of cell proliferation' was enriched in the upregulated genes. In addition, the G1/S cell cycle checkpoint regulator cyclin-dependent kinase inhibitor 1a (CDKN1A, also termed TP21) was downregulated in siMB LNCaP cells. These results indicated direct or indirect involvement of MB in TP21 expression and a possible induction of cell cycle arrest in the presence of MB. However, this scenario is most probably of a more complex nature, since a subset of cell cycle progression-associated genes such as cyclin B1 (CCNB1) and CCND1 were also downregulated in siMB LNCaP cells (Tables SI and SIII). Enrichment analyses also revealed that the GO categories 'cell motion' and 'cell migration' were significantly overrepresented among the upregulated genes of siMB LNCaP cells (Table II), including activated leukocyte cell adhesion molecule, CD9, RAC1, laminin subunit γ1 and thrombospondin (THBS1) under both O2 conditions (Tables SI and SIII). These observations strengthened our hypothesis that MB induced apoptosis and decreased the migratory and motion capacity of prostate cancer cells.

In breast cancer cells, genes associated with apoptosis were overrepresented in the sets of up- and downregulated MDA-MB468 cells under both O2 conditions (Table II), which made it difficult to discriminate the impact of MB on promoting or inhibiting apoptosis. The GO terms 'mitotic cell cycle', 'cell cycle process' and 'positive regulation of cell proliferation' were overrepresented in the downregulated genes of siMB cells, whereas only a limited number of negative regulators of cell proliferation were upregulated (including retinoic acid receptor responder 3, prostaglandin E synthase and CDKN2C; Tables SI and SIII). These results may imply increased proliferation rates in MB-positive breast cancer cells. In addition, the GO terms 'cell motion' and 'cell migration' were significantly overrepresented in the downregulated genes of siMB cells (Table II), as evidenced by the lower expression of THBS1, TNF receptor superfamily member 12A, vinculin, moesin and tensin 3 (Tables SI and SIII). Thus, the predictions made about the impact of MB on cell motion in hypoxic and normoxic breast cancer cells contradicted the results observed in prostate cancer cells.

In colon cancer cells, genes associated with apoptosis were also overrepresented in both up- and downregulated genes (Table II). The term 'cell cycle process' was overrepresented in the upregulated genes of hypoxic and normoxic siMB DLD-1 cells, including TPX2 microtubule nucleation factor, CDC20, non-SMC condensin I complex subunit D2, family with sequence similarity 83 member D, CCNB1 and CCNB2. In addition, the term 'cell cycle arrest' was overrepresented in the downregulated genes of hypoxic siMB samples (Table II) due to the downregulation of genes such as CDKN1C, CDKN1A, protein phosphatase 1 regulatory subunit 15A and DNA damage inducible transcript 3 (Tables SI and SIII). Therefore, the DLD-1 data suggested that MB expression may mediate the suppression of cell cycle progression in colon cancer cells, possibly via apoptotic pathways.

In line with the results of the prostate cancer cell line, the GO term 'cell motion' was also overrepresented in the down-regulated genes of normoxic siMB DLD-1 cells.

MB may impact on ROS and NO· homeostasis in epithelial cancer cell lines

In hypoxic and normoxic MB-knockdown breast cancer cells, the GO term 'regulation of NO· biosynthetic process' and other GO categories referring to the biosynthetic process of N-compounds were overrepresented in the downregulated genes, suggesting an increased production of NO· in hypoxic and normoxic MB-positive breast cancer cells (Table SIII). This dependency between MB expression and NO·-related GO terms was also observed in prostate and colon cancer cells, which suggested involvement of MB in NO· metabolism in all three cell types. All siMB cells also exhibited enrichment of the GO category 'response to ROS' in the downregulated genes irrespective of the O2 culture conditions. Therefore, MB may be involved in increasing ROS levels in all tested cell lines.

MB expression may affect the activity of key transcriptional regulators

To predict the differential activation of key transcriptional regulators in siMB vs. MB+ cells, the mRNA expression of their target genes was examined using bioinformatic IPA upstream regulator analysis (Table III). A subset of 40 transcription factors was suggested to be differentially active in at least one cell line at hypoxia or normoxia, with high Z-scores indicating strong activation, and low Z-scores indicating transcription factor inhibition. All siMB cells subjected to hypoxia and normoxic MDA-MB468 cells were predicted to exhibit decreased HIF1α activity compared with the MB+ controls. This was supported by the downregulation of HIF1α target genes in the siMB cell groups, including TP21 and nucleophosmin 1 in all cell lines, LDHA in LNCaP and MDA-MB468 cells, aldolase fructose-bisphosphate A and interleukin 8 in MDA-MB468 and DLD-1 cells, vascular endothelial growth factor A in LNCaP cells and HIF1A, solute carrier family 2 member 1 and carbonic anhydrase 9 in MDA-MB468 cells (Table SI). Thus, MB may activate HIF1α in breast cancer cells and, to a lesser extent, in prostate and colon cancer cells.

Table III

Ingenuity Pathway analysis of upstream regulator activity inferred from differential target gene expression.

Table III

Ingenuity Pathway analysis of upstream regulator activity inferred from differential target gene expression.

Upstream TFMB468 Hx
MB468 Nx
DLD Hx
DLD Nx
LNCaP Hx
LNCaP Nx
Entrez gene name
Fold chZFold chZFold chZFold chZFold chZFold chZ
ATF3−2.000Activating transcription factor 3
ATF4−1.0911.1862.000Activating transcription factor 4
CDKN2A0.4470.000Cyclin-dependent kinase inhibitor 2A
CTNNB1−0.218−0.811Catenin (cadherin-associated protein), beta 1, 88 kDa
EHF−0.3020.632ETS homologous factor
ERG2.000V-ets avian erythroblastosis virus E26 oncogene homolog
ETV5−1.000Ets variant 5
FOXM13.240−1.165Forkhead box M1
FOXO1−2.221−2.6211.4421,969−1.131Forkhead box O1
FOXO3−2.003−2.219−1.472−1.997Forkhead box O3
FOXO4−1.218Forkhead box O4
GATA4−0.351GATA binding protein 4
GLI10.114−0.671−1.400−1.154GLI family zinc finger 1
HAND2−0.351Heart and neural crest derivatives expressed 2
HIF1A−1.516−2.556−1.406−1.455−0.391−0.218Hypoxia inducible factor 1, alpha subunit
HLX1.0000.4470.3780.000H2.0-like homeobox
HOXD101.0001.633Homeobox D10
IRF3−2.208−1.972Interferon regulatory factor 3
JUN−1.474−1.622−1,653−1.000Jun proto-oncogene
KDM5B0.0000.832−2.864−0.8161.667Lysine (K)-specific demethylase 5B
MDM2−1.192−2.000−0.686MDM2 proto-oncogene, E3 ubiquitin protein ligase
MITF1.000 Microphthalmia-associated transcription factor
MYC0.1520.555−1,5680.849−1.6311.067V-myc avian myelocytomatosis viral oncogene homolog
MYOCD−0.612Myocardin
NANOG0.707Nanog homeobox
NEUROG11.0001.508Neurogenin 1
NFIX1.4070.655Nuclear factor I/X (CCAAT-binding transcription factor)
RELA−2.564−2.386−1.913−0.849V-rel avian reticuloendotheliosis viral oncogene homolog A
SATB1−1.494−0.5760.984−0.831−1.987−1.987SATB homeobox 1
SMAD4−1.000−1.987SMAD family member 4
SMARCA4−1.980SWI/SNF related, matrix associated, actin dependentregulator of chromatin
SNAI20.854Snail family zinc finger 2
SP1−1.000Sp1 transcription factor
STAT1−2.200Signal transducer and activator of transcription 1, 91 kDa
STAT3−1.653−0.8640.640Signal transducer and activator of transcription 3
TBX5−0.351T-box 5
TP53−2.756−2.568−1.480−0.6510.492Tumor protein p53
TP630.6501.3490.508−0.6540.060Tumor protein p63
TP73−1.230−0.970Tumor protein p73
YAP1−1.633−1.633Yes-associated protein 1

[i] TF, transcription factor; fold ch, fold-change; Z, z-score; siMB, cells transfected with small interfering RNA targeting myoglobin; MB+ cells transfected with scrambled small interfering RNA; Hx, hypoxia; Nx, normoxia.

IPA upstream activator analysis also suggested the involvement of p53 signaling in the three cancer cell lines (Table III). Of note, in our set up, only LNCaP cells encoded the TP53 wild-type-like Pro72Arg variant, whereas the MDA-MB468 and DLD-1 cells carried TP53 mutations Arg273His and Ser241Phe, respectively (Table SII), which affected the key residues of DNA-binding (37). RNA-Seq analysis of LNCaP cells demonstrated that MB+ was associated with the upregulation of p53 target genes including TP21, BCL2-interacting killer and heme oxygenase 1 (HMOX1) (Table SI), which suggested an activation of p53 signaling by MB in the prostate cancer model. In MDA-MB468 and DLD-1 cells expressing MB, even the mutant p53 was active, which was evidenced by the decreased expression of p63 target genes (Table III), possibly due to lowered p63 activity by the binding of mutated p53 (38,39). Accordingly, IPA analysis results suggested activation of p63 in MB+ hypoxic LNCaP cells expressing the wild-type-like TP53 mutation Pro72Arg (Table III). Another fitting observation noted for the breast cancer cell line was enhanced transcriptional activity predicted for the oncogenic factor RELA (Table III), which is known to be activated by mutant p53 (40).

In line with the observed metabolic shifts in MB+ cancer cells, the gluconeogenesis and adipogenesis regulator fork-head box O1 was another transcription factor predicted to be dysregulated in the three cell models (Table II).

Discussion

The respiratory protein MB, known for its prominent presence in myocytes, is ectopically expressed in several cell types and malignancies, including tumors of the breast, prostate, lung, colon, kidney, bone, and head and neck (18-26).

Only a limited number of hypothesis-driven experiments have been conducted thus far to elucidate the role of MB in cancer cells (28,41,42). The present study used the essentially unbiased approach of transcriptome analysis to reveal functional consequences of MB expression vs. RNAi-mediated MB knockdown in breast, prostate and colon cancer cells. Cells were cultured either at 1% O2 or at room air conditions for 72 h to reflect long-term hypoxia-adapted, poorly vascularized hypoxic tumor areas and aerobic tissues with oxygenated MB (35). By Illumina transcriptome sequencing and bioinformatic read mapping, differentially expressed genes between siMB and MB+ cells were identified for all cell lines at either oxygen level. Gene lists were subjected to analyses of gene set enrichment to identify dysregulated biological processes and differentially active gene regulators and pathways. As a main observation, these results demonstrated increased cellular activity of HIF1α signaling in MB+ vs. siMB epithelial cancer cells; HIF1α is the major transcription factor of the cellular hypoxia response (43). Multiple HIF1α target genes were differentially expressed in all cell lines cultured under hypoxia, and respective GO-categories were enriched. Of note, comparatively less HIF2α target genes were differentially expressed in MB+ vs. siMB cells, although HIF2α is generally considered to be more active following long-term hypoxia compared with HIF1α (44). Therefore, although hypoxia was applied for 72 h, the function of MB may be associated with the differential expression of HIF1α targets and may possibly be triggered by HIF1α activity. Supporting hypothetical interaction between MB and HIF1α, a positive correlation of MB mRNA or protein and HIF1α target gene expression was reported in breast, prostate and non-small cell lung cancer (21,22,24,29). MB levels were also increased in hypoxic, perinecrotic breast tumor regions (28). Additionally, in the present study, MB expression was upregulated in LNCaP, MDA-MB468 and DLD-1 cells cultured under 1% O2. This may be a result of HIF1α binding to a candidate hypoxia response enhancer element (27,28). Thus, transcription of MB in tumor cells may be triggered by a HIF-dependent transactivation. Based on the present gene expression data, a working model may be proposed, linking the presence of MB to HIF1α activity. A scheme of how this may be achieved is presented in Fig. 2. Deoxy-MB can produce NO· through its nitrite reductase activity (12). This enzymatic activity may be active in the cancer cell models used in the present study, since the GO category 'N-compound biosynthetic processes' was enriched in all MB+ cells. NO· inhibits the activity of the sensor protein prolylhydroxylase 2 (PHD2), which is the main PDH expressed in these cell models (45,46). This would lead to diminished hydroxylation and increased stabilization of the HIF1α protein, resulting in the increase of HIF1α target gene transcription (47,48), as observed in the present transcriptome data. Hypothetically, MB could counteract a fast decay of the HIF1α response by constantly producing NO· and decreasing PHD2 activity. As a formal alternative to this working model, MB may interfere with HIF1α signaling by modulating intracellular ROS levels. The ferrous iron of PHD2 can be oxidized by H2O2, which prevents HIF1α degradation and thus transiently increases HIF1α activity (49,50). Accordingly, the present transcriptome data demonstrated an overrepresentation of the GO category 'response to ROS' in all MB+ cells. In summary, MB may stabilize HIF1α and support the hypoxia response in epithelial cancer cells, which may reduce the formation of hypoxic niches, resulting in a tumor-suppressing phenotype. Of note, the activity of PHD2 could, to a certain extent, be replaced by PHD1 and PHD3 (51). However, the PHD2 expression levels were mostly higher compared with those of PHD1 and PHD3 based on their RPKM values. In addition, a strong correlation of PHD2 and MB expression was previously reported in breast cancer (29), making it the prime candidate to potentially hydroxylate HIF1α in an MB/NO-dependent manner. In agreement with the model established in the present study, MB-generated NO· would simultaneously also inhibit PHD1 and PHD3 activity (45).

A second major result of the present transcriptome comparison of MB+ and siMB cancer cells involved changes in metabolism. Breast and prostate MB+ cells revealed upregulation of genes involved in the energy-producing part of glycolysis and of LDHA, which converts pyruvate into L-lactate and regenerates NAD+ from NADH+ H+ to sustain anaerobic substrate flux (52). This was in line with the enhanced HIF1α signaling described above, since the upregulated genes are known HIF1α targets (53). In contrast to the breast and prostate cancer cell lines, hypoxic MB+ colon cancer cells exhibited downregulation of glycolysis-related genes and LDHA. GO term enrichment analyses of hypoxic colon cancer cells revealed that MB may contribute to enhanced cholesterol biosynthesis, previously described to occur in this cell type (54). HMGCS1, which induces cholesterol synthesis, was upregulated in MB+ DLD-1 cells, whereas FOXO1, a negative regulator of HMGCS1 expression and thus cholesterol synthesis (55), was inactive. The involvement of MB in cholesterol metabolism appears to be unique to the colon cancer cell line cultured under hypoxia. By contrast, the breast cancer line indicated a specific contribution of MB to the metabolism of unsaturated fatty acids at normoxia, as indicated by enrichment of the associated GO terms. This supported previous in vitro studies demonstrating that oxy-MB was able to bind palmitic and oleic acid with physiological binding constants (13-15), potentially indicating a non-canonical role of the globin in the intracellular transport of unsaturated fatty acids. The observation that all three epithelial cancer cell line transcriptomes indicated different metabolic shifts in response to MB knockdown suggests that additional cell type-specific properties need to be considered in order to understand the distinct metabolic role of MB in different cancer entities.

A third major finding of the present study was that the GO categories associated with negative or positive p53-mediated regulation of apoptosis and programmed cell death were overrepresented in all three MB+ cell lines. Since the three cell models differ in their p53 genotype, they require separate interpretation of the transcriptome data.

LNCaP prostate cancer cells express the wild-type-like TP53 mutation Pro72Arg, which has a higher binding affinity to p73 and thus an increased potential to induce apoptosis (56,57). In the prostate cancer cell line, a multitude of p53 target genes were upregulated in MB+ cells, including the elevated expression of the p53 target gene TP21, which controls the G1/S cell cycle checkpoint and inhibits cell cycle progression (58). The activity of p21 is enhanced by increased ROS levels (59), as implicated by the results of the GO term enrichment. Elevated ROS levels, along with increased p53 activity, may trigger oxidative damage-induced cell death and, as a result, selectively kill cancer cells (60). As added indirect evidence for the presence of ROS in the present cell model, increased levels of HMOX1 were observed in MB+ LNCaP; HMOX1 is an enzyme that can be induced by oxidative stress and p53, and whose presence is also associated with cell cycle arrest and reduced cancer cell viability (60). This hypothetical p53/p21-mediated tumor-suppressing effect was further substantiated by the overrepresentation of GO categories associated with cell motion and migration in siMB vs. MB+ cells. The transcriptome data of the prostate cancer model were thus in agreement with the ameliorated survival prognoses of patients with MB-positive prostate tumors (24). A hypothetical working model explaining the potential impact of MB on p53 activity is presented in Fig. 3.

As proposed previously in the HIF1α scenario, deoxy-MB may enhance p53 action by producing NO· in hypoxic LNCaP cells which, in turn, could induce the phosphorylation of p53Pro72Arg and inhibit its nuclear export by MDM2 proto-oncogene, resulting in nuclear retention and subsequent activation (61). Augmentation of p53Pro72Arg signaling may further be maintained by stabilized HIF1α or by increased ROS levels (62,63), both of which may also be associated with the action of deoxy-MB (Fig. 3).

In contrast to the prostate cancer line, the breast cancer cell model MDA-MB468 encoded the gain-of-function TP53 mutation Arg273His, which impairs p53 target site binding (37). Despite the inability to directly drive p53 target gene expression, mutated p53 could function by interacting with other proteins. p53Arg273His may interact with and inactivate the tumor suppressor p63 (39) in the cell model used in the present study, as indicated by the observed downregulation of p63 target genes in MB+ breast cancer cells. This may lead to enhanced cell survival and decreased apoptosis, which has been previously described in TP53Arg273His vs. TP53-knockdown MDA-MB468 cells (38). In addition, mutated p53 may act as a co-factor of the RELA proto-oncogene/NF-κB and STAT3 complex to induce the transcription of NF-κB-regulated genes (40). The present transcriptome results suggested increased activity of p53Arg273His, RELA and STAT3 in MB+ breast cancer cells. Specifically for p53Arg273His expressing cell models, a tumor-promoting effect of MB may be hypothesized, which could be maintained, as proposed previously in terms of wild-type-like p53, by MB-mediated activation and stabilization of p53Arg273His via increased cellular NO·, ROS and HIF1α levels (63).

In addition, GO-terms associated with cell migration, motion, and adhesion were enriched in the genes upregulated in MB+ MDA-MB468 cells. This finding was in agreement with a previous study, which revealed increased migratory potential, viability and proliferation rates in normoxia- and hypoxia-cultured MB+ MDA-MB468 cells (28), thus experimentally validating our bioinformatics data. These in silico and in vitro results from the MDA-MB468 breast cancer cell model are contradictory to the beneficial prognostic outcome associated with MB positivity in breast tumors (21). However, the published Kaplan-Meier survival data lacked information about the patient p53 status. It may be speculated that the observed prognostic correlation mainly referred to wild-type-p53 cancer cases, but may result in the opposite outcome if analyzing the prognostic impact of MB in p53-gain-of-function mutated tumors. An alternative explanation is the strong positive correlation of MB with ER-expression in breast cancer, which itself is associated with better patient outcome. Of note, MB expression had additive prognostic value to ER alone, which raises the possibility of influences additional to the p53 status (21).

Transcription factor activity analyses also suggested p53 to be active in MB+ DLD-1 colon cancer cells, which encoded the TP53 mutation Ser241Phe. Despite impaired target gene binding (37), p53Ser241Phe may also hypothetically function via interaction with other proteins. In analogy to MDA-MB468 cells, the predicted transcriptional activation of RELA in MB+, p53-mutated colon cancer cells suggested that the RELA/NF-κB/STAT3 complex may be active and participate in the transcription of NF-κB target genes (40).

However, in contrast to a tumor-promoting role of MB in p53-mutated DLD-1 cells, upstream regulator analyses indicated significantly decreased activity of FOXM1 in MB+ DLD-1 cells, possibly indicating increased G2/M arrest (64). In addition, the transcription factor FOXO3, which acts as a tumor suppressor by transcribing genes involved in cell cycle arrest and DNA repair (65), was predicted to be more active in MB+ DLD-1 cells. FOXO3 can be trans-activated by HIF1α and induced by metabolic stress and increased ROS levels (66), i.e., the conditions and transcription factors indicated to be active with MB expression. Although the differential activity of mutant p53 and RELA suggested tumor propagation by MB in DLD-1, the results also indicated that MB expression may indirectly intervene with cell cycle progression.

As an alternative scenario to a p53-driven cell cycle arrest, oxy-MB was recently reported to decrease cell proliferation by inducing mitochondrial fusion in p53-wild-type, as well as in p53-gain-of-function-mutated and p53-loss-of-function-mutated cell lines (42). In this network, oxy-MB was described to produce ROS, which increased parkin oxidation and inhibited the degradation of mitofusin 1 and 2. Thus, MB-positivity was linked to an increase in mitochondrial fusion, resulting in G1/S phase arrest. Although the results of the present study also demonstrated enrichment of genes associated with corresponding GO terms, the genes specifically involved in ROS/parkin-driven cell cycle arrest were not differentially expressed in any of the studied MB+ vs. siMB cell transcriptomes.

In summary, the present study performed the first transcriptome comparison of epithelial cancer cell models with endogenous vs. knocked-down levels of MB mRNA expression. The results of the present study revealed distinct changes in gene expression following MB knockdown and suggested novel working hypotheses to integrate MB function into metabolic pathways and gene networks of epithelial cancer cells. Non-canonical enzymatic functions, which have previously been reported for MB in myocytes, seamlessly fit to explain a number of the present RNA-Seq results. Thus, important starting points were identified for future experimental work on the role of MB in tumors, e.g., by carefully considering the p53 status when choosing cell models. The proposed ability of MB to fine-tune levels of NO· and ROS signaling molecules in epithelial cancer cells, if substantiated by further biochemical research, may significantly improve our understanding of cancer cell physiology.

Supplementary Data

Acknowledgments

Not applicable.

Funding

The present study was supported by Deutsche Forschungsgemeinschaft (grant no. Ha2103/10-1) and intramural grants by Johannes Gutenberg University Mainz (Stufe I) and the Johannes Gutenberg University Center for Computer Studies (CSM/SRFN). An Ingenuity Pathway Analysis license was provided by the Naturwissenschaftlich-medizinisches Forschungszentrum (NMFZ) and the University Medical Center Mainz. For cell culture, further financial support was received from von-Muralt Stiftung für Kleintiere, Switzerland.

Availability of data and materials

The datasets generated and analyzed during the current study are available in the EBI repository as study PRJEB31329 (https://www.ebi.ac.uk/ena/data/view/PRJEB31329).

Authors' contributions

AB processed and analyzed the RNA-Seq and wrote the manuscript. TN analyzed the RNA-Seq data. DG and MAA conducted cell culture experiments. GK and JF inspected the data and revised the manuscript. TAG conducted cell culture experiments, inspected the data and revised the manuscript. TH interpreted the data and wrote the final manuscript. All authors read and approved the final manuscript.

Ethics approval and consent to participate

Not applicable.

Patient consent for publication

Not applicable.

Competing interests

The authors declare that they have no competing interests.

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February-2020
Volume 45 Issue 2

Print ISSN: 1107-3756
Online ISSN:1791-244X

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Spandidos Publications style
Bicker A, Nauth T, Gerst D, Aboouf MA, Fandrey J, Kristiansen G, Gorr TA and Hankeln T: The role of myoglobin in epithelial cancers: Insights from transcriptomics. Int J Mol Med 45: 385-400, 2020.
APA
Bicker, A., Nauth, T., Gerst, D., Aboouf, M.A., Fandrey, J., Kristiansen, G. ... Hankeln, T. (2020). The role of myoglobin in epithelial cancers: Insights from transcriptomics. International Journal of Molecular Medicine, 45, 385-400. https://doi.org/10.3892/ijmm.2019.4433
MLA
Bicker, A., Nauth, T., Gerst, D., Aboouf, M. A., Fandrey, J., Kristiansen, G., Gorr, T. A., Hankeln, T."The role of myoglobin in epithelial cancers: Insights from transcriptomics". International Journal of Molecular Medicine 45.2 (2020): 385-400.
Chicago
Bicker, A., Nauth, T., Gerst, D., Aboouf, M. A., Fandrey, J., Kristiansen, G., Gorr, T. A., Hankeln, T."The role of myoglobin in epithelial cancers: Insights from transcriptomics". International Journal of Molecular Medicine 45, no. 2 (2020): 385-400. https://doi.org/10.3892/ijmm.2019.4433