Preconditioning with repetitive electromagnetic stimulation enhances activity of bone marrow mesenchymal stem cells from elderly patients through Erk1/2 via nitric oxide

Nam,Seungwoo; Kim,Suna; Yoon,Kangjun; Hong,Hyun  Sook; Son,Youngsook

doi:10.3892/ijmm.2019.4450

Preconditioning with repetitive electromagnetic stimulation enhances activity of bone marrow mesenchymal stem cells from elderly patients through Erk1/2 via nitric oxide

Authors:
- Seungwoo Nam
- Suna Kim
- Kangjun Yoon
- Hyun Sook Hong
- Youngsook Son
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Affiliations: Department of Genetic Engineering, College of Life Science and Graduate School of Biotechnology, Kyung Hee University, Yongin, Gyeonggi‑do 446‑701, Republic of Korea, St. Peter's Hospital, Seoul 135‑859, Republic of Korea, Department of Biomedical Science and Technology, Graduate School, Kyung Hee University, Republic of Korea
Published online on: December 30, 2019 https://doi.org/10.3892/ijmm.2019.4450
Pages: 678-686

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Abstract

Use of bone marrow aspirate (BMA) is a clinically advantageous cell therapeutic that bypasses the need for elaborate ex vivo cell culturing. However, a low level of bone marrow‑mesenchymal stem cells (BM‑MSCs) in the BMA and weak survival rate of these cells post‑transplantation entails an insufficient efficacy in vivo. Moreover, stem cell activity in BMA is impaired by age or background diseases. Thus, in order to enrich the BM‑MSC pool and improve cell survival, novel cell preconditioning technologies are required. In this study, it has been revealed that the pretreatment of repetitive electromagnetic stimulation (rEMS) is capable of enhancing fibroblastic colony‑forming units and cell proliferation in the BM‑MSCs, possibly via transient nitric oxide production and extracellular signal regulated kinase 1/2 activation. Notably, this effect was more apparent in stem cells isolated from older patients than from young patients. Furthermore, the rEMS‑pretreated cells showed ~53% higher cell survival, compared with the untreated cells, after cell transplantation in mice with no signs of tumorigenesis. Collectively, transient rEMS preconditioning could be utilized to enhance the activity of stem cells and thus, application of rEMS preconditioning to stem cells isolated from older patients is expected to improve the therapeutic effect of stem cells.

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