Open Access

PLAG1 silencing promotes cell chemosensitivity in ovarian cancer via the IGF2 signaling pathway

  • Authors:
    • Wei Huang
    • Bi‑Rong Li
    • Hao Feng
  • View Affiliations

  • Published online on: January 8, 2020     https://doi.org/10.3892/ijmm.2020.4459
  • Pages: 703-714
  • Copyright: © Huang et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

Ovarian cancer (OC) is one of the most lethal gynecological diseases. Novel prognostic biomarkers and therapeutic targets for OC are urgently required. The aim of this study was to investigate the mechanisms that govern how pleomorphic adenoma gene 1 (PLAG1) influences the biological processes and chemosensitivity of OC cells via the insulin‑like growth factor‑2 (IGF2) signaling pathway. Differentially expressed genes in OC were selected based on bioinformatics data. OC and adjacent tissue specimen were collected, followed by the determination of the expression of PLAG1 and IGF2 signaling pathway‑associated genes. The regulatory mechanisms of PLAG1 in OC cells were analyzed following treatment with pcDNA or small interfering RNA (siRNA), and included the assessment of cell proliferation, migration, invasion and cisplatin resistance. PLAG1 was identified as an upregulated gene in OC. OC tissues exhibited increased expression of PLAG1 and IGF2 compared with the controls. Moreover, PLAG1 was observed to positively regulate the IGF2 signaling pathway. The siRNA‑mediated silencing of PLAG1 resulted in decreased expression of IGF2, IGF1 receptor and insulin receptor substrate 1, as well as inhibited proliferation, migration, invasion and cisplatin resistance of OC cells. Furthermore, the effect of PLAG1 was dependent on IGF2. PLAG1 may therefore be considered as a possible target for the treatment of OC.
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March 2020
Volume 45 Issue 3

Print ISSN: 1107-3756
Online ISSN:1791-244X

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APA
Huang, W., Li, B., & Feng, H. (2020). PLAG1 silencing promotes cell chemosensitivity in ovarian cancer via the IGF2 signaling pathway. International Journal of Molecular Medicine, 45, 703-714. https://doi.org/10.3892/ijmm.2020.4459
MLA
Huang, W., Li, B., Feng, H."PLAG1 silencing promotes cell chemosensitivity in ovarian cancer via the IGF2 signaling pathway". International Journal of Molecular Medicine 45.3 (2020): 703-714.
Chicago
Huang, W., Li, B., Feng, H."PLAG1 silencing promotes cell chemosensitivity in ovarian cancer via the IGF2 signaling pathway". International Journal of Molecular Medicine 45, no. 3 (2020): 703-714. https://doi.org/10.3892/ijmm.2020.4459