Ligustrazine ameliorates acute kidney injury through downregulation of NOD2‑mediated inflammation

Jiang,Guosheng; Xin,Rui; Yuan,Wendan; Zhang,Lixia; Meng,Xianghui; Sun,Wangnan; Han,Huirong; Hou,Yun; Wang,Lin; Du,Pengchao

doi:10.3892/ijmm.2020.4464

Ligustrazine ameliorates acute kidney injury through downregulation of NOD2‑mediated inflammation

Authors:
- Guosheng Jiang
- Rui Xin
- Wendan Yuan
- Lixia Zhang
- Xianghui Meng
- Wangnan Sun
- Huirong Han
- Yun Hou
- Lin Wang
- Pengchao Du
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Affiliations: School of Basic Medical Sciences, Binzhou Medical University, Yantai, Shandong 264003, P.R. China, Center for Reproductive Medicine, National Research Center for Assisted Reproductive Technology and Reproductive Genetics, Shandong University, The Key Laboratory of Reproductive Endocrinology, Shandong University, Ministry of Education, Shandong Provincial Key Laboratory of Reproductive Medicine, Jinan, Shandong 250021, P.R. China, Microwave Treatment Department, Central Hospital of Zibo (GaoQing Branch Courts), Zibo, Shandong 256300, P.R. China, Department of Anesthesiology and Shandong Provincial Medicine and Health Key Laboratory of Clinical Anesthesia, Weifang Medical University, Weifang, Shandong 261053, P.R. China, Department of Gerontology, The Second Hospital of Shandong University, Jinan, Shandong 250000, P.R. China
Published online on: January 10, 2020 https://doi.org/10.3892/ijmm.2020.4464
Pages: 731-742
Copyright: © Jiang et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

Ligustrazine has been used to alleviate clinical acute kidney injury (AKI); however, the underlying molecular mechanisms are poorly understood. In order to further elucidate the molecular mechanism underlying its occurrence, the role of nucleotide‑binding oligomerization domain‑containing 2 (NOD2) in AKI was investigated in the present study, and the results indicated that ligustrazine exerts an important protective effect against AKI in vivo by inhibiting the upregulation of NOD2 expression and reducing apoptosis of kidney cells following ischemia/reperfusion injury in rat models. Furthermore, the inhibitory role of ligustrazine on the upregulation of NOD2 and apoptosis of kidney cells induced by CoCl2 and oxygen and glucose deprivation followed by reoxygenation was investigated in in vitro experiments. The effect of ligustrazine on NOD2 downregulation was partially blocked by inhibiting autophagy. To the best of our knowledge, the results of the present study are the first to provide evidence that ligustrazine can inhibit NOD2‑mediated inflammation to protect against renal injury, which may be in part attributed to the induction of autophagy. These findings may help design and develop new approaches and therapeutic strategies for AKI to prevent the deterioration of renal function.

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