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SNAIL expression correlates with the translocation of syndecan‑1 intracellular domain into the nucleus in prostate cancer cell lines

  • Authors:
    • Nancy Farfán
    • Octavio Orellana‑Serradell
    • Daniela Herrera
    • Dominique Chrzanowsky
    • Paulina Cubillos
    • Gabriel Marín
    • Antonio Antonio García De Herreros
    • Enrique A. Castellón
    • Héctor R. Contreras

  • View Affiliations

  • Published online on: February 5, 2020     https://doi.org/10.3892/ijmm.2020.4488
  • Pages: 1073-1080

  • Copyright: © Farfán et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

Zinc finger protein SNAI1 (SNAIL) and zinc finger protein SNAI2 (SLUG) transcription factors promote epithelial‑mesenchymal transition, a process through which epithelial cells acquire a mesenchymal phenotype, increasing their migratory and invasive properties. In prostate cancer (PCa) progression, increased expression levels of SNAIL and SLUG have been described. In advanced PCa, a decrease in the cell surface proteoglycan syndecan‑1 (SDC‑1), which has a role in cell‑to‑extracellular matrix adhesion, has been observed. Notably, SDC‑1 nuclear location has been observed in mesenchymal cancers. The present study aimed to determine if SNAIL and SLUG may be associated with the nuclear location of SDC‑1 in PCa. To determine the location of SDC‑1, antibodies against its intracellular domain (ID) or extracellular domain (ED) were used in benign prostatic hyperplasia (BPH) and PCa samples with high Gleason scores. Only ID‑SDC‑1 was located in the cell nuclei in advanced PCa samples, but not in the BPH samples. ED‑SDC‑1 was located in the cell membrane and cytoplasm, exhibiting decreased levels in PCa in comparison with those in BPH. Furthermore, LNCaP and PC3 PCa cell lines with ectopic SNAIL expression exhibited nuclear ID‑SDC‑1. No change was observed in the ED‑SDC‑1 levels, and maintained its location in the cell membrane and cytoplasm. SLUG induced no change in ID‑SDC‑1 location. At the protein level, an association between SNAIL and nuclear ID‑SDC‑1 was observed. In conclusion, the results of the present study demonstrated that nuclear ID‑SDC‑1 localization was associated with SNAIL expression in PCa cell lines.
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April-2020
Volume 45 Issue 4

Print ISSN: 1107-3756
Online ISSN:1791-244X

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  • CiteScore Rank: Q1: #35/328 Biochemistry, Genetics and Molecular Biology
  • Source Normalized Impact per Paper (SNIP): 0.994
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Spandidos Publications style
Farfán N, Orellana‑Serradell O, Herrera D, Chrzanowsky D, Cubillos P, Marín G, Antonio García De Herreros A, Castellón EA and Contreras HR: SNAIL expression correlates with the translocation of syndecan‑1 intracellular domain into the nucleus in prostate cancer cell lines. Int J Mol Med 45: 1073-1080, 2020
APA
Farfán, N., Orellana‑Serradell, O., Herrera, D., Chrzanowsky, D., Cubillos, P., Marín, G. ... Contreras, H.R. (2020). SNAIL expression correlates with the translocation of syndecan‑1 intracellular domain into the nucleus in prostate cancer cell lines. International Journal of Molecular Medicine, 45, 1073-1080. https://doi.org/10.3892/ijmm.2020.4488
MLA
Farfán, N., Orellana‑Serradell, O., Herrera, D., Chrzanowsky, D., Cubillos, P., Marín, G., Antonio García De Herreros, A., Castellón, E. A., Contreras, H. R."SNAIL expression correlates with the translocation of syndecan‑1 intracellular domain into the nucleus in prostate cancer cell lines". International Journal of Molecular Medicine 45.4 (2020): 1073-1080.
Chicago
Farfán, N., Orellana‑Serradell, O., Herrera, D., Chrzanowsky, D., Cubillos, P., Marín, G., Antonio García De Herreros, A., Castellón, E. A., Contreras, H. R."SNAIL expression correlates with the translocation of syndecan‑1 intracellular domain into the nucleus in prostate cancer cell lines". International Journal of Molecular Medicine 45, no. 4 (2020): 1073-1080. https://doi.org/10.3892/ijmm.2020.4488