Downregulation of lncRNA MALAT1 suppresses abnormal proliferation of small intestinal epithelial stem cells through miR‑129‑5p expression in diabetic mice

  • Authors:
    • Ti‑Dong Shan
    • Zi‑Bin Tian
    • Yue‑Ping Jiang
  • View Affiliations

  • Published online on: February 6, 2020     https://doi.org/10.3892/ijmm.2020.4492
  • Pages: 1250-1260
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Abstract

The problems caused by diabetes mellitus (DM) and its related complications are gaining increasing attention. In our previous study, the abnormal proliferation of small intestinal epithelial cells (IECs) were observed in diabetic mice. However, little is known regarding the potential underlying mechanism. In the present study, the abnormal proliferation of IECs in DM and the marked upregulation of metastasis associated lung adenocarcinoma transcript 1 (MALAT1) was observed. Additionally, knockdown of MALAT1 significantly reduced abnormal IESC proliferation in DM mice. Bioinformatics analysis and luciferase reporter assays revealed that microRNA (miR)‑129‑5p was directly targeted by MALAT1. Moreover, the results of the bioinformatics prediction and luciferase assays demonstrated that MALAT1 directly interacted with SRY‑box 9 (SOX9). Furthermore, MALAT1 silencing was observed to attenuate the abnormal proliferation of IESCs through the SOX9‑mediated WNT/β‑catenin signaling pathway. Knockdown of MALAT1 downregulated SOX9 expression by binding to miR‑129‑5p, thereby inhibiting the abnormal proliferation of IESCs via the WNT/β‑catenin signaling pathway.
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April-2020
Volume 45 Issue 4

Print ISSN: 1107-3756
Online ISSN:1791-244X

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Spandidos Publications style
Shan TD, Tian ZB and Jiang YP: Downregulation of lncRNA MALAT1 suppresses abnormal proliferation of small intestinal epithelial stem cells through miR‑129‑5p expression in diabetic mice. Int J Mol Med 45: 1250-1260, 2020
APA
Shan, T., Tian, Z., & Jiang, Y. (2020). Downregulation of lncRNA MALAT1 suppresses abnormal proliferation of small intestinal epithelial stem cells through miR‑129‑5p expression in diabetic mice. International Journal of Molecular Medicine, 45, 1250-1260. https://doi.org/10.3892/ijmm.2020.4492
MLA
Shan, T., Tian, Z., Jiang, Y."Downregulation of lncRNA MALAT1 suppresses abnormal proliferation of small intestinal epithelial stem cells through miR‑129‑5p expression in diabetic mice". International Journal of Molecular Medicine 45.4 (2020): 1250-1260.
Chicago
Shan, T., Tian, Z., Jiang, Y."Downregulation of lncRNA MALAT1 suppresses abnormal proliferation of small intestinal epithelial stem cells through miR‑129‑5p expression in diabetic mice". International Journal of Molecular Medicine 45, no. 4 (2020): 1250-1260. https://doi.org/10.3892/ijmm.2020.4492