Open Access

Antitumor effects and mechanisms of pyropheophorbide‑α methyl ester‑mediated photodynamic therapy on the human osteosarcoma cell line MG‑63

  • Authors:
    • Yanyang Chen
    • Hang Yin
    • Yong Tao
    • Shenxi Zhong
    • Haoyang Yu
    • Jianxiao Li
    • Zhibiao Bai
    • Yunsheng Ou
  • View Affiliations

  • Published online on: February 10, 2020     https://doi.org/10.3892/ijmm.2020.4494
  • Pages: 971-982
  • Copyright: © Chen et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

Photodynamic therapy (PDT) is a promising treatment for osteosarcoma, and pyropheophorbide‑α methyl ester (MPPa) is a second‑generation photosensitizer for tumor treatment. The present study aimed to determine the efficacy and possible mechanisms of MPPa‑PDT in the treatment of osteosarcoma MG‑63 cells. Flow cytometry and western blotting were used to detect cell cycle‑related indicators Cyclin D1, Cyclin E, Cyclin A and Cyclin B1. Cell migration and invasion abilities were detected using wound‑healing and Transwell chamber assays. Cellular endoplasmic reticulum stress (ERS), autophagy and apoptosis‑related indicators were detected by flow cytometry and western blotting. The results demonstrated that MPPa‑PDT blocked the MG‑63 cell cycle and inhibited cell migration and invasion. Additionally, MPPa‑PDT inhibited the activation of the Akt/mammalian target of rapamycin (mTOR) pathway. MG‑63 cells underwent ERS‑induced apoptosis following MPPa‑PDT treatment. Pretreatment with the mTOR phosphorylation inhibitor rapamycin affected the autophagy of MPPa‑PDT‑induced osteosarcoma MG‑63 cells and enhanced apoptosis through targeting mTOR.
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April-2020
Volume 45 Issue 4

Print ISSN: 1107-3756
Online ISSN:1791-244X

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Spandidos Publications style
Chen Y, Yin H, Tao Y, Zhong S, Yu H, Li J, Bai Z and Ou Y: Antitumor effects and mechanisms of pyropheophorbide‑α methyl ester‑mediated photodynamic therapy on the human osteosarcoma cell line MG‑63. Int J Mol Med 45: 971-982, 2020
APA
Chen, Y., Yin, H., Tao, Y., Zhong, S., Yu, H., Li, J. ... Ou, Y. (2020). Antitumor effects and mechanisms of pyropheophorbide‑α methyl ester‑mediated photodynamic therapy on the human osteosarcoma cell line MG‑63. International Journal of Molecular Medicine, 45, 971-982. https://doi.org/10.3892/ijmm.2020.4494
MLA
Chen, Y., Yin, H., Tao, Y., Zhong, S., Yu, H., Li, J., Bai, Z., Ou, Y."Antitumor effects and mechanisms of pyropheophorbide‑α methyl ester‑mediated photodynamic therapy on the human osteosarcoma cell line MG‑63". International Journal of Molecular Medicine 45.4 (2020): 971-982.
Chicago
Chen, Y., Yin, H., Tao, Y., Zhong, S., Yu, H., Li, J., Bai, Z., Ou, Y."Antitumor effects and mechanisms of pyropheophorbide‑α methyl ester‑mediated photodynamic therapy on the human osteosarcoma cell line MG‑63". International Journal of Molecular Medicine 45, no. 4 (2020): 971-982. https://doi.org/10.3892/ijmm.2020.4494