Open Access

ptsI gene in the phosphotransfer system is a potential target for developing a live attenuated Salmonella vaccine

  • Authors:
    • Yong Zhi
    • Shun Mei Lin
    • Ki Bum Ahn
    • Hyun Jung Ji
    • Hui‑Chen Guo
    • Sangryeol Ryu
    • Ho Seong Seo
    • Sangyong Lim
  • View Affiliations

  • Published online on: February 18, 2020     https://doi.org/10.3892/ijmm.2020.4505
  • Pages: 1327-1340
  • Copyright: © Zhi et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

Salmonella enterica serovar Typhimurium causes invasive non‑typhoidal Salmonella diseases in animals and humans, resulting in a high mortality rate and huge economic losses globally. As the prevalence of antibiotic‑resistant Salmonella has been increasing, vaccination is thought to be the most effective and economical strategy to manage salmonellosis. The present study aimed to investigate whether dysfunction in the phosphoenolpyruvate:carbohydrate phosphotransferase system (PTS), which is critical for carbon uptake and survival in macrophages, may be adequate to generate Salmonella‑attenuated vaccine strains. A Salmonella strain (KST0555) was generated by deleting the ptsI gene from the PTS and it was revealed that this auxotrophic mutant was unable to efficiently utilize predominant carbon sources during infection (glucose and glycerol), reduced its invasion and replication capacity in macrophages, and significantly (P=0.0065) lowered its virulence in the setting of a mouse colitis model, along with a substantially decreased intestinal colonization and invasiveness compared with its parent strain. The reverse transcription‑quantitative PCR results demonstrated that the virulence genes in Salmonella pathogenicity island-1 (SPI-1) and -2 (SPI-2) and the motility of KST0555 were all downregulated compared with its parent strain. Finally, it was revealed that when mice were immunized orally with live KST0555, Salmonella‑specific humoral and cellular immune responses were effectively elicited, providing protection against Salmonella infection. Thus, the present promising data provides a strong rationale for the advancement of KST0555 as a live Salmonella vaccine candidate and ptsI as a potential target for developing a live attenuated bacterial vaccine strain.

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May 2020
Volume 45 Issue 5

Print ISSN: 1107-3756
Online ISSN:1791-244X

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APA
Zhi, Y., Lin, S.M., Ahn, K.B., Ji, H.J., Guo, H., Ryu, S. ... Lim, S. (2020). ptsI gene in the phosphotransfer system is a potential target for developing a live attenuated Salmonella vaccine. International Journal of Molecular Medicine, 45, 1327-1340. https://doi.org/10.3892/ijmm.2020.4505
MLA
Zhi, Y., Lin, S. M., Ahn, K. B., Ji, H. J., Guo, H., Ryu, S., Seo, H. S., Lim, S."ptsI gene in the phosphotransfer system is a potential target for developing a live attenuated Salmonella vaccine". International Journal of Molecular Medicine 45.5 (2020): 1327-1340.
Chicago
Zhi, Y., Lin, S. M., Ahn, K. B., Ji, H. J., Guo, H., Ryu, S., Seo, H. S., Lim, S."ptsI gene in the phosphotransfer system is a potential target for developing a live attenuated Salmonella vaccine". International Journal of Molecular Medicine 45, no. 5 (2020): 1327-1340. https://doi.org/10.3892/ijmm.2020.4505