MicroRNA‑431 inhibits the expression of surfactant proteins through the BMP4/activin/TGF‑β signaling pathway by targeting SMAD4

  • Authors:
    • Yan‑Qing Shen
    • Zhi‑Dan Bao
    • Jing‑Jing Pan
    • Xiao‑Nan Mao
    • Rui Cheng
    • Xiao‑Guang Zhou
    • Xiao‑Yu Zhou
    • Yang Yang
  • View Affiliations

  • Published online on: February 24, 2020     https://doi.org/10.3892/ijmm.2020.4511
  • Pages: 1571-1582
Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )
Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )


Abstract

The synthesis and secretion of surfactant proteins (SPs) is an important sign of lung maturation. Furthermore, the morbidity of lung developmental diseases, including respiratory distress syndrome and bronchopulmonary dysplasia which are mainly caused by immature lung development and lack of SPs, is increasing. As is well known, multiple microRNAs (miRs/miRNAs) are able to influence lung development via numerous different signaling pathways. However, few studies examine the association between the miRNAs and lung developmental diseases. A previous study has demonstrated that miR‑431 was significantly (F=33.49; P<0.001) downregulated in the lung tissues of Sprague‑Dawley rats at 3 time points, embryonic day 19, embryonic day 21 and postnatal day 3. The present study reported that the regulation of miR‑431 may influence the expression of SPs. Thus, the further potential mechanisms of miR‑431 in negatively regulating lung development were examined in the present study. Stable A549 cell lines overexpressing or knocking down SMAD family member 4 (SMAD4) transfected with miR‑431 overexpressed or knocked down, and their control groups were established. Subsequently, the expression of bone morphogenetic protein 4 (BMP4), SMAD4 and SPs (SP‑A, SP‑B and SP‑C) at the RNA and protein levels were validated respectively by reverse transcription quantitative PCR and western blotting. miR‑431 exhibited a decreased expression, while BMP4 and SPs exhibited increased expression at the mRNA and protein levels in the SMAD4 knockdown group. Meanwhile, the expression of SPs were reduced in the SMAD4‑knockdown group via overexpressing miR‑431 and increased in the SMAD4‑overexpression group via inhibiting miR‑431. The present results indicate that SMAD4 negatively regulates the expression of SPs, and that miR‑431 negatively regulates the expression of SPs through inhibiting the BMP4/activin/transforming growth factor‑β signaling pathway by targeting SMAD4.

Related Articles

Journal Cover

May 2020
Volume 45 Issue 5

Print ISSN: 1107-3756
Online ISSN:1791-244X

Sign up for eToc alerts

Recommend to Library

Copy and paste a formatted citation
x
APA
Shen, Y., Bao, Z., Pan, J., Mao, X., Cheng, R., Zhou, X. ... Yang, Y. (2020). MicroRNA‑431 inhibits the expression of surfactant proteins through the BMP4/activin/TGF‑β signaling pathway by targeting SMAD4. International Journal of Molecular Medicine, 45, 1571-1582. https://doi.org/10.3892/ijmm.2020.4511
MLA
Shen, Y., Bao, Z., Pan, J., Mao, X., Cheng, R., Zhou, X., Zhou, X., Yang, Y."MicroRNA‑431 inhibits the expression of surfactant proteins through the BMP4/activin/TGF‑β signaling pathway by targeting SMAD4". International Journal of Molecular Medicine 45.5 (2020): 1571-1582.
Chicago
Shen, Y., Bao, Z., Pan, J., Mao, X., Cheng, R., Zhou, X., Zhou, X., Yang, Y."MicroRNA‑431 inhibits the expression of surfactant proteins through the BMP4/activin/TGF‑β signaling pathway by targeting SMAD4". International Journal of Molecular Medicine 45, no. 5 (2020): 1571-1582. https://doi.org/10.3892/ijmm.2020.4511