LncRNA BCAR4 promotes liver cancer progression by upregulating ANAPC11 expression through sponging miR‑1261

Zhang,Yu; Zhou,Hongyan

doi:10.3892/ijmm.2020.4586

LncRNA BCAR4 promotes liver cancer progression by upregulating ANAPC11 expression through sponging miR‑1261

Authors:
- Yu Zhang
- Hongyan Zhou
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Affiliations: Department of General Surgery, Shanxi Provincial People's Hospital, Taiyuan, Shanxi 030012, P.R. China, Department of Gastroenterology, Taiyuan Second People's Hospital, Taiyuan, Shanxi 030012, P.R. China
Published online on: April 22, 2020 https://doi.org/10.3892/ijmm.2020.4586
Pages: 159-166
Copyright: © Zhang et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

Liver cancer is a malignant tumor that occurs in the liver and can be divided into primary and secondary liver cancer. Long non‑coding RNA (lncRNA) breast cancer anti‑estrogen resistance 4 (BCAR4) has been demonstrated to promote the development of various types of cancer. However, the function of lncRNA BCAR4 in liver cancer remains unclear. In the present study, the expression of lncRNA BCAR4 was notably elevated in liver cancer compared with adjacent non‑tumor tissues. Functional in vitro assays demonstrated that knockdown of lncRNA BCAR4 inhibited the proliferation, migration and invasion of Huh‑7 cells. In addition, lncRNA BCAR4 was demonstrated to directly bind to microRNA (miR)‑1261, and miR‑1261 expression negatively correlated with the expression of lncRNA BCAR4. Through bioinformatics analysis, lncRNA BCAR4 was predicted to target anaphase‑promoting complex subunit 11 (ANAPC11) through miR‑1261. In addition, the results demonstrated that lncRNA BCAR4 increased the expression of ANAPC11 by inhibiting miR‑1261 expression. Consistently, overexpression of ANAPC11 or inhibition of miR‑1261 significantly rescued liver cancer cell proliferation induced by knockdown of lncRNA BCAR4. Collectively, the results of the present study demonstrated that lncRNA BCAR4 may promote liver cancer development by directly binding to miR‑1261 and targeting ANAPC11.

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1	Lazăr DC, Avram MF, Romoșan I, Văcariu V, Goldiș A and Cornianu M: Malignant hepatic vascular tumors in adults: Characteristics, diagnostic difficulties and current management. World J Clin Oncol. 10:110–135. 2019. View Article : Google Scholar
2	Turley EA, Veiseh M, Radisky DC and Bissell MJ: Mechanisms of disease: Epithelial-mesenchymal transition-does cellular plasticity fuel neoplastic progression? Nat Clin Pract Oncol. 5:280–290. 2008. View Article : Google Scholar : PubMed/NCBI
3	van Tienderen GS, Groot Koerkamp B, IJzermans JNM, van der Laan LJW and Verstegen MMA: Recreating tumour complexity in a dish: Organoid models to study liver cancer cells and their extracellular environment. Cancers (Basel). 11:pii: E1706. 2019. View Article : Google Scholar
4	Kang M, Sang Y, Gu H, Zheng L, Wang L, Liu C, Shi Y, Shao A, Ding G, Chen S, et al: Long noncoding RNAs POLR2E rs3787016 C/T and HULC rs7763881 A/C polymorphisms are associated with decreased risk of esophageal cancer. Tumour Biol. 36:6401–6408. 2015. View Article : Google Scholar : PubMed/NCBI
5	Klec C, Gutschner T, Panzitt K and Pichler M: Involvement of long non-coding RNA HULC (highly up-regulated in liver cancer) in pathogenesis and implications for therapeutic intervention. Expert Opin Ther Targets. 23:177–186. 2019. View Article : Google Scholar : PubMed/NCBI
6	Kong X, Duan Y, Sang Y, Li Y, Zhang H, Liang Y, Liu Y, Zhang N and Yang Q: LncRNA-CDC6 promotes breast cancer progression and function as ceRNA to target CDC6 by sponging microRNA-215. J Cell Physiol. 234:9105–9117. 2019. View Article : Google Scholar
7	Ouyang S, Zheng X, Zhou X, Chen Z, Yang X and Xie M: LncRNA BCAR4 promotes colon cancer progression via activating Wnt/β-catenin signaling. Oncotarget. 8:92815–92826. 2017. View Article : Google Scholar : PubMed/NCBI
8	Xing Z, Park PK, Lin C and Yang L: LncRNA BCAR4 wires up signaling transduction in breast cancer. RNA Biol. 12:681–689. 2015. View Article : Google Scholar : PubMed/NCBI
9	Li N, Gao WJ and Liu NS: LncRNA BCAR4 promotes proliferation, invasion and metastasis of non-small cell lung cancer cells by affecting epithelial-mesenchymal transition. Eur Rev Med Pharmacol Sci. 21:2075–2086. 2017.PubMed/NCBI
10	Yin XH, Jin YH, Cao Y, Wong Y, Weng H, Sun C, Deng JH and Zeng XT: Development of a 21-miRNA signature associated with the prognosis of patients with bladder cancer. Front Oncol. 9:7292019. View Article : Google Scholar : PubMed/NCBI
11	Hu Z, Gao S, Lindberg D, Panja D, Wakabayashi Y, Li K, Kleinman JE, Zhu J and Li Z: Temporal dynamics of miRNAs in human DLPFC and its association with miRNA dysregulation in schizophrenia. Transl Psychiatry. 9:1962019. View Article : Google Scholar : PubMed/NCBI
12	Angius A, Uva P, Pira G, Muroni MR, Sotgiu G, Saderi L, Uleri E, Caocci M, Ibba G, Cesaraccio MR, et al: Integrated analysis of miRNA and mRNA endorses a twenty miRNAs signature for colorectal carcinoma. Int J Mol Sci. 20:pii: E4067. 2019. View Article : Google Scholar
13	Yen MC, Yeh IJ, Liu KT, Jian SF, Lin CJ, Tsai MJ and Kuo PL: Next-generation sequencing predicts interaction network between miRNA and target genes in lipoteichoic acid-stimulated human neutrophils. Int J Mol Med. 44:1436–1446. 2019.PubMed/NCBI
14	He JH, Li BX, Han ZP, Zou MX, Wang L, Lv YB, Zhou JB, Cao MR, Li YG and Zhang JZ: Snail-activated long non-coding RNA PCA3 up-regulates PRKD3 expression by miR-1261 sponging, thereby promotes invasion and migration of prostate cance cells. Tumour Biol. Oct 14–2016.Epub ahead of print. View Article : Google Scholar
15	Wei H, Pan L, Tao D and Li R: Circular RNA circZFR contributes to papillary thyroid cancer cell proliferation and invasion by sponging miR-1261 and facilitating C8orf4 expression. Biochem Biophys Res Commun. 503:56–61. 2018. View Article : Google Scholar : PubMed/NCBI
16	Zhang F, Mai SR, Cao FP, Cao CX and Zhang L: MiR-1261/circ-PTPRZ1/PAK1 pathway regulates glioma cell growth and invasion. Human Cell. 32:540–547. 2019. View Article : Google Scholar : PubMed/NCBI
17	Shi YJ and Huo KK: Knockdown expression of Apc11 leads to cell-cycle distribution reduction in G2/M phase. Genet Mol Res. 11:2814–2822. 2012. View Article : Google Scholar : PubMed/NCBI
18	Drouet Y, Treilleux I, Viari A, Léon S, Devouassoux-Shisheboran M, Voirin N, de la Fouchardière C, Manship B, Puisieux A, Lasset C and Moyret-Lalle C: Integrated analysis highlights APC11 protein expression as a likely new independent predictive marker for colorectal cancer. Sci Rep. 8:73862018. View Article : Google Scholar : PubMed/NCBI
19	Wang G, Fang X, Han M, Wang X and Huang Q: MicroRNA-493-5p promotes apoptosis and suppresses proliferation and invasion in liver cancer cells by targeting VAMP2. Int J Mol Med. 41:1740–1748. 2018.PubMed/NCBI
20	Xin X, Wu M, Meng Q, Wang C, Lu Y, Yang Y, Li X, Zheng Q, Pu H, Gui X, et al: Long noncoding RNA HULC accelerates liver cancer by inhibiting PTEN via autophagy cooperation to miR15a. Mol Cancer. 17:942018. View Article : Google Scholar : PubMed/NCBI
21	Li R, Zhu H, Yang D, Xia J and Zheng Z: Long noncoding RNA lncBRM promotes proliferation and invasion of colorectal cancer by sponging miR-204-3p and upregulating TPT1. Biochem Biophys Res Commun. 508:1259–1263. 2019. View Article : Google Scholar
22	Francica G and Borzio M: Status of, and strategies for improving, adherence to HCC screening and surveillance. J Hepatocell Carcinoma. 6:131–141. 2019. View Article : Google Scholar : PubMed/NCBI
23	Viveiros P, Riaz A, Lewandowski RJ and Mahalingam D: Current state of liver-directed therapies and combinatory approaches with systemic therapy in hepatocellular carcinoma (HCC). Cancers (Basel). 11:pii: E1085. 2019. View Article : Google Scholar
24	Gao Q, Zhang G, Zheng Y, Yang Y, Chen C, Xia J, Liang L, Lei C, Hu Y, Cai X, et al: SLC27A5 deficiency activates NRF2/TXNRD1 pathway by increased lipid peroxidation in HCC. Cell Death Differ. 27:1086–1104. 2020. View Article : Google Scholar
25	Feld J: Update on the risk of primary and recurrent HCC With the use of DAA therapy for HCV infection. Gastroenterol Hepatol (N Y). 15:303–306. 2019.
26	Fang G, Zhang P, Liu J, Zhang X, Zhu X, Li R and Wang H: Inhibition of GSK-3β activity suppresses HCC malignant phenotype by inhibiting glycolysis via activating AMPK/mTOR signaling. Cancer Lett. 463:11–26. 2019. View Article : Google Scholar : PubMed/NCBI
27	Shigeta K, Datta M, Hato T, Kitahara S, Chen IX, Matsui A, Kikuchi H, Mamessier E, Aoki S, Ramjiawan RR, et al: Dual programmed death receptor-1 and vascular endothelial growth factor receptor-2 blockade promotes vascular normalization and enhances antitumor immune responses in hepatocellular carcinoma. Hepatology. Aug 5–2019.Epub ahead of print. PubMed/NCBI
28	Ju JX, Zeng QJ, Xu EJ, He XQ, Tan L, Huang QN, Li K and Zheng RQ: Intraprocedural contrast-enhanced ultrasound-CT/MR fusion imaging assessment in HCC thermal ablation to reduce local tumor progression: Compared with routine contrast-enhanced ultrasound. Int J Hyperthermia. 36:785–793. 2019. View Article : Google Scholar : PubMed/NCBI
29	Mao LH, Chen SY, Li XQ, Xu F, Lei J, Wang QL, Luo LY, Cao HY, Ge X, Ran T, et al: LncRNA-LALR1 upregulates small nucleolar RNA SNORD72 to promote growth and invasion of hepatocellular carcinoma. Aging (Albany NY). 12:4527–4546. 2020. View Article : Google Scholar
30	Yan D, Jin F and Lin Y: lncRNA HAND2-AS1 inhibits liver cancer cell proliferation and migration by upregulating SOCS5 to inactivate the JAK-STAT pathway. Cancer Biother Radiopharm. 35:143–152. 2020. View Article : Google Scholar : PubMed/NCBI
31	Ouyang S, Zhou X, Chen Z, Wang M, Zheng X and Xie M: LncRNA BCAR4, targeting to miR-665/STAT3 signaling, maintains cancer stem cells stemness and promotes tumorigenicity in colorectal cancer. Cancer Cell Int. 19:722019. View Article : Google Scholar : PubMed/NCBI
32	Godinho MF, Wulfkuhle JD, Look MP, Sieuwerts AM, Sleijfer S, Foekens JA, Petricoin EF III, Dorssers LC and van Agthoven T: BCAR4 induces antioestrogen resistance but sensitises breast cancer to lapatinib. Br J Cancer. 107:947–955. 2012. View Article : Google Scholar : PubMed/NCBI
33	Yang H, Yan L, Sun K, Sun X, Zhang X, Cai K and Song T: lncRNA BCAR4 increases viability, invasion, and migration of non-small cell lung cancer cells by targeting glioma-associated oncogene 2 (GLI2). Oncol Res. 27:359–369. 2019. View Article : Google Scholar
34	Wei Y, Wang Z, Zong Y, Deng D, Chen P and Lu J: LncRNA MFI2-AS1 promotes HCC progression and metastasis by acting as a competing endogenous RNA of miR-134 to upregulate FOXM1 expression. Biomed Pharmacother. 125:1098902020. View Article : Google Scholar : PubMed/NCBI
35	Moyret-Lalle C, Drouet Y and Puisieux A: The catalytic subunit of the anaphase-promoting complex, APC11, is involved in CIN⁺ CRC progression. Med Sci (Paris). 35:118–122. 2019.In French. View Article : Google Scholar