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miR‑187‑3p inhibitor attenuates cerebral ischemia/reperfusion injury by regulating Seipin‑mediated autophagic flux

  • Authors:
    • Zhenkui Ren
    • Peng Xie
    • Ju Lv
    • Yumei Hu
    • Zhizhong  Guan
    • Ling Chen
    • Wenfeng Yu
  • View Affiliations / Copyright

    Affiliations: Key Laboratory of Endemic and Ethnic Diseases, Ministry of Education, School of Basic Medical Science, Guizhou Medical University, Guiyang, Guizhou 550004, P.R. China, Laboratory of Reproductive Medicine, Department of Physiology, Nanjing Medical University, Nanjing, Jiangsu 210029, P.R. China
    Copyright: © Ren et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 1051-1062
    |
    Published online on: June 16, 2020
       https://doi.org/10.3892/ijmm.2020.4642
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Abstract

MicroRNAs (miRNAs/miRs) have been reported to affect ischemia/reperfusion (I/R)‑induced cerebral damage. miRNAs cause post‑transcriptional gene silencing by binding to the protein‑coding sequence (CDS) of mRNAs. Seipin has a potential role in regulating autophagic flux. The present study investigated the involvement of miR‑187‑3p in Seipin expression, autophagic flux and apoptosis in vitro, as well as the underlying mechanism, using PC12 cells exposed to oxygen‑glucose deprivation/reoxygenation (OGD/R), which mimicked the process of I/R. In comparison with control PC12 cells, OGD/R caused an increase in the level of miR‑187‑3p and a decrease in Seipin protein levels without changes in the level of Seipin mRNA. Using bioinformatics analysis, it was identified that miR‑187‑3p could bind to the CDS of Seipin. miR‑187‑3p inhibitor attenuated the reduction in Seipin protein expression in OGD/R‑treated PC12 cells. Following OGD/R, autophagic flux was reduced and apoptosis was enhanced, which were attenuated by inhibition of miR‑187‑3p. Compared with OGD/R‑treated PC12 cells, Seipin knockdown further impaired autophagic flux and promoted neuronal apoptosis, which were insensitive to inhibition of miR‑187‑3p. Furthermore, treatment with miR‑187‑3p inhibitor could decrease the infarction volume in a rat model of middle cerebral artery occlusion/reperfusion. The present findings indicated that miR‑187‑3p inhibitor attenuated ischemia‑induced cerebral damage by rescuing Seipin expression to improve autophagic flux.
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Copy and paste a formatted citation
Spandidos Publications style
Ren Z, Xie P, Lv J, Hu Y, Guan Z, Chen L and Yu W: miR‑187‑3p inhibitor attenuates cerebral ischemia/reperfusion injury by regulating Seipin‑mediated autophagic flux. Int J Mol Med 46: 1051-1062, 2020.
APA
Ren, Z., Xie, P., Lv, J., Hu, Y., Guan, Z., Chen, L., & Yu, W. (2020). miR‑187‑3p inhibitor attenuates cerebral ischemia/reperfusion injury by regulating Seipin‑mediated autophagic flux. International Journal of Molecular Medicine, 46, 1051-1062. https://doi.org/10.3892/ijmm.2020.4642
MLA
Ren, Z., Xie, P., Lv, J., Hu, Y., Guan, Z., Chen, L., Yu, W."miR‑187‑3p inhibitor attenuates cerebral ischemia/reperfusion injury by regulating Seipin‑mediated autophagic flux". International Journal of Molecular Medicine 46.3 (2020): 1051-1062.
Chicago
Ren, Z., Xie, P., Lv, J., Hu, Y., Guan, Z., Chen, L., Yu, W."miR‑187‑3p inhibitor attenuates cerebral ischemia/reperfusion injury by regulating Seipin‑mediated autophagic flux". International Journal of Molecular Medicine 46, no. 3 (2020): 1051-1062. https://doi.org/10.3892/ijmm.2020.4642
Copy and paste a formatted citation
x
Spandidos Publications style
Ren Z, Xie P, Lv J, Hu Y, Guan Z, Chen L and Yu W: miR‑187‑3p inhibitor attenuates cerebral ischemia/reperfusion injury by regulating Seipin‑mediated autophagic flux. Int J Mol Med 46: 1051-1062, 2020.
APA
Ren, Z., Xie, P., Lv, J., Hu, Y., Guan, Z., Chen, L., & Yu, W. (2020). miR‑187‑3p inhibitor attenuates cerebral ischemia/reperfusion injury by regulating Seipin‑mediated autophagic flux. International Journal of Molecular Medicine, 46, 1051-1062. https://doi.org/10.3892/ijmm.2020.4642
MLA
Ren, Z., Xie, P., Lv, J., Hu, Y., Guan, Z., Chen, L., Yu, W."miR‑187‑3p inhibitor attenuates cerebral ischemia/reperfusion injury by regulating Seipin‑mediated autophagic flux". International Journal of Molecular Medicine 46.3 (2020): 1051-1062.
Chicago
Ren, Z., Xie, P., Lv, J., Hu, Y., Guan, Z., Chen, L., Yu, W."miR‑187‑3p inhibitor attenuates cerebral ischemia/reperfusion injury by regulating Seipin‑mediated autophagic flux". International Journal of Molecular Medicine 46, no. 3 (2020): 1051-1062. https://doi.org/10.3892/ijmm.2020.4642
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