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Article Open Access

Knockdown of SNHG16 suppresses the proliferation and induces the apoptosis of leukemia cells via miR‑193a‑5p/CDK8

Retraction in: /10.3892/ijmm.2024.5389
  • Authors:
    • Meihua Piao
    • Li Zhang
  • View Affiliations / Copyright

    Affiliations: Clinical Laboratory, Yanbian University Hospital (Yanbian Hospital), Yanji, Jilin 133000, P.R. China, Department of Neonatology, Weinan Maternal and Child Health Hospital, Weinan, Shaanxi 714000, P.R. China
    Copyright: © Piao et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 1175-1185
    |
    Published online on: July 8, 2020
       https://doi.org/10.3892/ijmm.2020.4671
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Abstract

Although small nucleolar RNA host gene 16 (SNHG16) is known to exhibit auxo‑action in certain types of tumor, its role in leukemia remains unclear. The present study analyzed the role and mechanisms of action of SNHG16 in leukemia cells in order to identify therapeutic targets for this disease. Reverse transcription‑quantitative polymerase chain reaction (RT‑qPCR) was performed to determine SNHG16 expression in human leukemia cell lines. Using TargetScan 7.2 and dual‑luciferase reporter assay, the target genes of SNHG16 were verified. Following the downregulation of the expression of SNHG16 or its target genes, Cell Counting kit‑8 (CCK‑8) assay was performed to examine the viability of the leukemia cells. In addition, flow cytometry was performed to analyze the cell apoptotic rates, and colony formation assays were used to determine the cell proliferative ability. RT‑qPCR and western blot analysis were used to determine the association between SNHG16 and its target genes. SNHG16 was found to be abnormally highly expressed in acute myeloblastic leukemia cell lines, the knockdown of which weakened the viability of the leukemia cells, suppressed cell proliferation and promoted cell apoptosis. miR‑193a‑5p could bind to SNHG16, and its target gene was CDK8. Moreover, the expression of miR‑193a‑5p increased with the decrease in SNHG16 expression, while the inhibition of miR‑193a‑5p promoted the expression of CDK8. The downregulation of miR‑193a‑5p enhanced the viability of the leukemia cells, accelerated cell cloning and reduced cell apoptosis, which was completely opposite to the effects observed with the silencing of CDK8. The knockdown of SNHG16 suppressed the viability of the leukemia cells, suppressed cell proliferation, and induced cell apoptosis by regulating miR‑193a‑5p/CDK8. Thus, SNHG16 may prove to be a potential therapeutic target for the treatment of leukemia.
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Copy and paste a formatted citation
Spandidos Publications style
Piao M and Zhang L: Knockdown of SNHG16 suppresses the proliferation and induces the apoptosis of leukemia cells via miR‑193a‑5p/CDK8 Retraction in /10.3892/ijmm.2024.5389. Int J Mol Med 46: 1175-1185, 2020.
APA
Piao, M., & Zhang, L. (2020). Knockdown of SNHG16 suppresses the proliferation and induces the apoptosis of leukemia cells via miR‑193a‑5p/CDK8 Retraction in /10.3892/ijmm.2024.5389. International Journal of Molecular Medicine, 46, 1175-1185. https://doi.org/10.3892/ijmm.2020.4671
MLA
Piao, M., Zhang, L."Knockdown of SNHG16 suppresses the proliferation and induces the apoptosis of leukemia cells via miR‑193a‑5p/CDK8 Retraction in /10.3892/ijmm.2024.5389". International Journal of Molecular Medicine 46.3 (2020): 1175-1185.
Chicago
Piao, M., Zhang, L."Knockdown of SNHG16 suppresses the proliferation and induces the apoptosis of leukemia cells via miR‑193a‑5p/CDK8 Retraction in /10.3892/ijmm.2024.5389". International Journal of Molecular Medicine 46, no. 3 (2020): 1175-1185. https://doi.org/10.3892/ijmm.2020.4671
Copy and paste a formatted citation
x
Spandidos Publications style
Piao M and Zhang L: Knockdown of SNHG16 suppresses the proliferation and induces the apoptosis of leukemia cells via miR‑193a‑5p/CDK8 Retraction in /10.3892/ijmm.2024.5389. Int J Mol Med 46: 1175-1185, 2020.
APA
Piao, M., & Zhang, L. (2020). Knockdown of SNHG16 suppresses the proliferation and induces the apoptosis of leukemia cells via miR‑193a‑5p/CDK8 Retraction in /10.3892/ijmm.2024.5389. International Journal of Molecular Medicine, 46, 1175-1185. https://doi.org/10.3892/ijmm.2020.4671
MLA
Piao, M., Zhang, L."Knockdown of SNHG16 suppresses the proliferation and induces the apoptosis of leukemia cells via miR‑193a‑5p/CDK8 Retraction in /10.3892/ijmm.2024.5389". International Journal of Molecular Medicine 46.3 (2020): 1175-1185.
Chicago
Piao, M., Zhang, L."Knockdown of SNHG16 suppresses the proliferation and induces the apoptosis of leukemia cells via miR‑193a‑5p/CDK8 Retraction in /10.3892/ijmm.2024.5389". International Journal of Molecular Medicine 46, no. 3 (2020): 1175-1185. https://doi.org/10.3892/ijmm.2020.4671
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