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Association between serum anti‑ASXL2 antibody levels and acute ischemic stroke, acute myocardial infarction, diabetes mellitus, chronic kidney disease and digestive organ cancer, and their possible association with atherosclerosis and hypertension

  • Authors:
    • Shu‑Yang Li
    • Yoichi Yoshida
    • Eiichi Kobayashi
    • Akihiko Adachi
    • Seiichiro Hirono
    • Tomoo Matsutani
    • Seiichiro Mine
    • Toshio Machida
    • Mikiko Ohno
    • Eiichiro Nishi
    • Yoshiro Maezawa
    • Minoru Takemoto
    • Koutaro Yokote
    • Kenichiro Kitamura
    • Makoto Sumazaki
    • Masaaki Ito
    • Hideaki Shimada
    • Hirotaka Takizawa
    • Koichi Kashiwado
    • Go Tomiyoshi
    • Natsuko Shinmen
    • Rika Nakamura
    • Hideyuki Kuroda
    • Xiao‑Meng Zhang
    • Hao Wang
    • Kenichiro Goto
    • Yasuo Iwadate
    • Takaki Hiwasa
  • View Affiliations / Copyright

    Affiliations: Department of Neurological Surgery, Graduate School of Medicine, Chiba University, Chiba 260‑8670, Japan, Department of Neurological Surgery, Chiba Cerebral and Cardiovascular Center, Chiba 290‑0512, Japan, Department of Cardiovascular Medicine, Graduate School of Medicine, Kyoto University, Kyoto 606‑8507, Japan, Department of Endocrinology, Hematology and Gerontology, Graduate School of Medicine, Chiba University, Chiba 260‑8670, Japan, Department of Internal Medicine 3, University of Yamanashi School of Medicine, Yamanashi 409‑3898, Japan, Department of Gastroenterological Surgery and Clinical Oncology, Toho University Graduate School of Medicine, Tokyo 143‑8541, Japan, Port Square Kashiwado Clinic, Kashiwado Memorial Foundation, Chiba 260‑0025, Japan, Department of Neurology, Kashiwado Hospital, Chiba 260‑8656, Japan, Department of Biochemistry and Genetics, Graduate School of Medicine, Chiba University, Chiba 260‑8670, Japan, Medical Project Division, Research Development Center, Fujikura Kasei Co., Saitama 340‑0203, Japan
    Copyright: © Li et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 1274-1288
    |
    Published online on: July 29, 2020
       https://doi.org/10.3892/ijmm.2020.4690
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Abstract

The aim of the present study was to identify novel antibody markers for the early diagnosis of atherosclerosis in order to improve the prognosis of patients at risk for acute ischemic stroke (AIS) and acute myocardial infarction (AMI). A first screening involved the serological identification of antigens by recombinant cDNA expression cloning and identified additional sex combs‑like 2 (ASXL2) as a target antigen recognized by serum IgG antibodies in the sera of patients with atherosclerosis. Antigens, including the recombinant glutathione S‑transferase‑fused ASXL2 protein and its synthetic peptide were then prepared to examine serum antibody levels. Amplified luminescence proximity homogeneous assay‑linked immunosorbent assay, which incorporates glutathione‑donor beads and anti‑human‑IgG‑acceptor beads, revealed significantly higher serum antibody levels against the ASXL2 protein and its peptide in the patients with AIS, diabetes mellitus, AMI, chronic kidney disease, esophageal squamous cell carcinoma, or colorectal carcinoma compared with those in healthy donors. The ASXL2 antibody levels were well associated with hypertension complication, but not with sex, body mass index, habitual smoking, or alcohol intake. These results suggest that the serum ASXL2 antibody marker can discriminate between hypertension‑induced atherosclerotic AIS and AMI, as well as a number of digestive organ cancers.
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Copy and paste a formatted citation
Spandidos Publications style
Li SY, Yoshida Y, Kobayashi E, Adachi A, Hirono S, Matsutani T, Mine S, Machida T, Ohno M, Nishi E, Nishi E, et al: Association between serum anti‑ASXL2 antibody levels and acute ischemic stroke, acute myocardial infarction, diabetes mellitus, chronic kidney disease and digestive organ cancer, and their possible association with atherosclerosis and hypertension. Int J Mol Med 46: 1274-1288, 2020.
APA
Li, S., Yoshida, Y., Kobayashi, E., Adachi, A., Hirono, S., Matsutani, T. ... Hiwasa, T. (2020). Association between serum anti‑ASXL2 antibody levels and acute ischemic stroke, acute myocardial infarction, diabetes mellitus, chronic kidney disease and digestive organ cancer, and their possible association with atherosclerosis and hypertension. International Journal of Molecular Medicine, 46, 1274-1288. https://doi.org/10.3892/ijmm.2020.4690
MLA
Li, S., Yoshida, Y., Kobayashi, E., Adachi, A., Hirono, S., Matsutani, T., Mine, S., Machida, T., Ohno, M., Nishi, E., Maezawa, Y., Takemoto, M., Yokote, K., Kitamura, K., Sumazaki, M., Ito, M., Shimada, H., Takizawa, H., Kashiwado, K., Tomiyoshi, G., Shinmen, N., Nakamura, R., Kuroda, H., Zhang, X., Wang, H., Goto, K., Iwadate, Y., Hiwasa, T."Association between serum anti‑ASXL2 antibody levels and acute ischemic stroke, acute myocardial infarction, diabetes mellitus, chronic kidney disease and digestive organ cancer, and their possible association with atherosclerosis and hypertension". International Journal of Molecular Medicine 46.4 (2020): 1274-1288.
Chicago
Li, S., Yoshida, Y., Kobayashi, E., Adachi, A., Hirono, S., Matsutani, T., Mine, S., Machida, T., Ohno, M., Nishi, E., Maezawa, Y., Takemoto, M., Yokote, K., Kitamura, K., Sumazaki, M., Ito, M., Shimada, H., Takizawa, H., Kashiwado, K., Tomiyoshi, G., Shinmen, N., Nakamura, R., Kuroda, H., Zhang, X., Wang, H., Goto, K., Iwadate, Y., Hiwasa, T."Association between serum anti‑ASXL2 antibody levels and acute ischemic stroke, acute myocardial infarction, diabetes mellitus, chronic kidney disease and digestive organ cancer, and their possible association with atherosclerosis and hypertension". International Journal of Molecular Medicine 46, no. 4 (2020): 1274-1288. https://doi.org/10.3892/ijmm.2020.4690
Copy and paste a formatted citation
x
Spandidos Publications style
Li SY, Yoshida Y, Kobayashi E, Adachi A, Hirono S, Matsutani T, Mine S, Machida T, Ohno M, Nishi E, Nishi E, et al: Association between serum anti‑ASXL2 antibody levels and acute ischemic stroke, acute myocardial infarction, diabetes mellitus, chronic kidney disease and digestive organ cancer, and their possible association with atherosclerosis and hypertension. Int J Mol Med 46: 1274-1288, 2020.
APA
Li, S., Yoshida, Y., Kobayashi, E., Adachi, A., Hirono, S., Matsutani, T. ... Hiwasa, T. (2020). Association between serum anti‑ASXL2 antibody levels and acute ischemic stroke, acute myocardial infarction, diabetes mellitus, chronic kidney disease and digestive organ cancer, and their possible association with atherosclerosis and hypertension. International Journal of Molecular Medicine, 46, 1274-1288. https://doi.org/10.3892/ijmm.2020.4690
MLA
Li, S., Yoshida, Y., Kobayashi, E., Adachi, A., Hirono, S., Matsutani, T., Mine, S., Machida, T., Ohno, M., Nishi, E., Maezawa, Y., Takemoto, M., Yokote, K., Kitamura, K., Sumazaki, M., Ito, M., Shimada, H., Takizawa, H., Kashiwado, K., Tomiyoshi, G., Shinmen, N., Nakamura, R., Kuroda, H., Zhang, X., Wang, H., Goto, K., Iwadate, Y., Hiwasa, T."Association between serum anti‑ASXL2 antibody levels and acute ischemic stroke, acute myocardial infarction, diabetes mellitus, chronic kidney disease and digestive organ cancer, and their possible association with atherosclerosis and hypertension". International Journal of Molecular Medicine 46.4 (2020): 1274-1288.
Chicago
Li, S., Yoshida, Y., Kobayashi, E., Adachi, A., Hirono, S., Matsutani, T., Mine, S., Machida, T., Ohno, M., Nishi, E., Maezawa, Y., Takemoto, M., Yokote, K., Kitamura, K., Sumazaki, M., Ito, M., Shimada, H., Takizawa, H., Kashiwado, K., Tomiyoshi, G., Shinmen, N., Nakamura, R., Kuroda, H., Zhang, X., Wang, H., Goto, K., Iwadate, Y., Hiwasa, T."Association between serum anti‑ASXL2 antibody levels and acute ischemic stroke, acute myocardial infarction, diabetes mellitus, chronic kidney disease and digestive organ cancer, and their possible association with atherosclerosis and hypertension". International Journal of Molecular Medicine 46, no. 4 (2020): 1274-1288. https://doi.org/10.3892/ijmm.2020.4690
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