Open Access

Ginsenoside Rh2 inhibits HeLa cell energy metabolism and induces apoptosis by upregulating voltage‑dependent anion channel 1

  • Authors:
    • Ying Liu
    • Jiawen Wang
    • Juhui Qiao
    • Shichao Liu
    • Siming Wang
    • Daqing Zhao
    • Xueyuan Bai
    • Meichen Liu
  • View Affiliations

  • Published online on: September 14, 2020     https://doi.org/10.3892/ijmm.2020.4725
  • Pages: 1695-1706
  • Copyright: © Liu et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

20(S)‑Ginsenoside Rh2 [20(S)‑GRh2], one of the main active components of Panax ginseng, induces apoptosis in a wide range of cancer cell types. The present study found that 20(S)‑GRh2 reduces mitochondrial membrane potential, decreases adenosine triphosphate generation and induces reactive oxygen species in HeLa cervical cancer cells. In addition, 20(S)‑GRh2 activated mitochondrion‑dependent apoptosis and inhibited both mitochondrial oxidative phosphorylation and glycolysis in HeLa cells. It was found that voltage‑dependent anion channel 1 (VDAC1) expression was significantly upregulated by 20(S)‑GRh2 treatment, while hexokinase 2 expression was downregulated and segregated from the mitochondria. Furthermore, 20(S)‑GRh2 promoted Bax transport from the cytoplasm to the mitochondria, and knockdown of VDAC1 inhibited Bax transport and apoptosis. These results suggest that VDAC1 is a novel target of 20(S)‑GRh2. The present study provides a better understanding of the mechanistic link between cervical cancer metabolism and growth control, and these results may facilitate the development of new treatments for cervical cancer.
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November-2020
Volume 46 Issue 5

Print ISSN: 1107-3756
Online ISSN:1791-244X

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Spandidos Publications style
Liu Y, Wang J, Qiao J, Liu S, Wang S, Zhao D, Bai X and Liu M: Ginsenoside Rh2 inhibits HeLa cell energy metabolism and induces apoptosis by upregulating voltage‑dependent anion channel 1. Int J Mol Med 46: 1695-1706, 2020
APA
Liu, Y., Wang, J., Qiao, J., Liu, S., Wang, S., Zhao, D. ... Liu, M. (2020). Ginsenoside Rh2 inhibits HeLa cell energy metabolism and induces apoptosis by upregulating voltage‑dependent anion channel 1. International Journal of Molecular Medicine, 46, 1695-1706. https://doi.org/10.3892/ijmm.2020.4725
MLA
Liu, Y., Wang, J., Qiao, J., Liu, S., Wang, S., Zhao, D., Bai, X., Liu, M."Ginsenoside Rh2 inhibits HeLa cell energy metabolism and induces apoptosis by upregulating voltage‑dependent anion channel 1". International Journal of Molecular Medicine 46.5 (2020): 1695-1706.
Chicago
Liu, Y., Wang, J., Qiao, J., Liu, S., Wang, S., Zhao, D., Bai, X., Liu, M."Ginsenoside Rh2 inhibits HeLa cell energy metabolism and induces apoptosis by upregulating voltage‑dependent anion channel 1". International Journal of Molecular Medicine 46, no. 5 (2020): 1695-1706. https://doi.org/10.3892/ijmm.2020.4725