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Article Open Access

Resveratrol contributes to the inhibition of liver fibrosis by inducing autophagy via the microRNA‑20a‑mediated activation of the PTEN/PI3K/AKT signaling pathway

  • Authors:
    • Lili Zhu
    • Qiuju Mou
    • Yinghui Wang
    • Zixin Zhu
    • Mingliang Cheng
  • View Affiliations / Copyright

    Affiliations: Department of Blood Transfusion, The Affiliated Hospital of Guizhou Medical University, Guiyang, Guizhou 550004, P.R. China, Department of Blood Transfusion, The Affiliated Baiyun Hospital of Guizhou Medical University, Guiyang, Guizhou 550004, P.R. China, Graduate School, Guizhou Medical University, Guiyang, Guizhou 550004, P.R. China, Department of Infectious Diseases, The Affiliated Hospital of Guizhou Medical University, Guiyang, Guizhou 550004, P.R. China
    Copyright: © Zhu et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 2035-2046
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    Published online on: October 9, 2020
       https://doi.org/10.3892/ijmm.2020.4748
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Abstract

Liver fibrosis (LF) is a healing response to wounds resulting in liver injury that can cause liver failure or even cancer without functional prevention. Resveratrol (RSV) has been suggested to exert biological effects against various human diseases. MicroRNA‑20a (miRNA/miR‑20a) has been shown to promote disease progression. The present study aimed to assess the mechanisms through which RSV induces autophagy and activates the miR‑20a‑mediated phosphatase and tensin homolog (PTEN)/PI3K/AKT signaling pathway in LF. First, a rat model of carbon tetrachloride (CCL4)‑induced LF and a cell model of platelet‑derived growth factor (PDGF)‑BB‑stimulated HSC‑T6 cells were established for use in subsequent experiments. Subsequently, RSV at a range of concentrations was injected into the model rats with LF. Indicators related to liver injury, oxidative stress and fibrosis were determined in the rats with LF. The RSV‑treated HSC‑T6 cells were subjected to transfection with miR‑20a mimic and PTEN overexpression plasmid to assess the levels of liver injury and LF. A dual‑luciferase reporter gene assay was performed to verify the binding sites between PTEN and miR‑20a. RSV was found to alleviate LF in rats, and autophagy was enhanced in the rats with LF following RSV treatment. Furthermore, the activation of the PTEN/PI3K/AKT axis attenuated LF, which was reversed by transfection with miR‑20a mimic. RSV reversed the inhibitory effects of miR‑20a on PTEN expression, reducing miR‑20a expression and promoting PTEN, PI3K and p‑AKT protein expression, thus attenuating LF. On the whole, the present study demonstrates that RSV induces autophagy and activates the miR‑20a‑mediated PTEN/PI3K/AKT signaling pathway to attenuate LF. These findings may lead to the development of potential therapeutic strategies for LF.
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Copy and paste a formatted citation
Spandidos Publications style
Zhu L, Mou Q, Wang Y, Zhu Z and Cheng M: Resveratrol contributes to the inhibition of liver fibrosis by inducing autophagy via the microRNA‑20a‑mediated activation of the PTEN/PI3K/AKT signaling pathway. Int J Mol Med 46: 2035-2046, 2020.
APA
Zhu, L., Mou, Q., Wang, Y., Zhu, Z., & Cheng, M. (2020). Resveratrol contributes to the inhibition of liver fibrosis by inducing autophagy via the microRNA‑20a‑mediated activation of the PTEN/PI3K/AKT signaling pathway. International Journal of Molecular Medicine, 46, 2035-2046. https://doi.org/10.3892/ijmm.2020.4748
MLA
Zhu, L., Mou, Q., Wang, Y., Zhu, Z., Cheng, M."Resveratrol contributes to the inhibition of liver fibrosis by inducing autophagy via the microRNA‑20a‑mediated activation of the PTEN/PI3K/AKT signaling pathway". International Journal of Molecular Medicine 46.6 (2020): 2035-2046.
Chicago
Zhu, L., Mou, Q., Wang, Y., Zhu, Z., Cheng, M."Resveratrol contributes to the inhibition of liver fibrosis by inducing autophagy via the microRNA‑20a‑mediated activation of the PTEN/PI3K/AKT signaling pathway". International Journal of Molecular Medicine 46, no. 6 (2020): 2035-2046. https://doi.org/10.3892/ijmm.2020.4748
Copy and paste a formatted citation
x
Spandidos Publications style
Zhu L, Mou Q, Wang Y, Zhu Z and Cheng M: Resveratrol contributes to the inhibition of liver fibrosis by inducing autophagy via the microRNA‑20a‑mediated activation of the PTEN/PI3K/AKT signaling pathway. Int J Mol Med 46: 2035-2046, 2020.
APA
Zhu, L., Mou, Q., Wang, Y., Zhu, Z., & Cheng, M. (2020). Resveratrol contributes to the inhibition of liver fibrosis by inducing autophagy via the microRNA‑20a‑mediated activation of the PTEN/PI3K/AKT signaling pathway. International Journal of Molecular Medicine, 46, 2035-2046. https://doi.org/10.3892/ijmm.2020.4748
MLA
Zhu, L., Mou, Q., Wang, Y., Zhu, Z., Cheng, M."Resveratrol contributes to the inhibition of liver fibrosis by inducing autophagy via the microRNA‑20a‑mediated activation of the PTEN/PI3K/AKT signaling pathway". International Journal of Molecular Medicine 46.6 (2020): 2035-2046.
Chicago
Zhu, L., Mou, Q., Wang, Y., Zhu, Z., Cheng, M."Resveratrol contributes to the inhibition of liver fibrosis by inducing autophagy via the microRNA‑20a‑mediated activation of the PTEN/PI3K/AKT signaling pathway". International Journal of Molecular Medicine 46, no. 6 (2020): 2035-2046. https://doi.org/10.3892/ijmm.2020.4748
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