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International Journal of Molecular Medicine
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Article Open Access

Knockdown of long non‑coding RNA CCAT2 suppresses the progression of thyroid cancer by inhibiting the Wnt/β‑catenin pathway

  • Authors:
    • Suping Xin
    • Xinhua Ye
  • View Affiliations / Copyright

    Affiliations: Department of Endocrinology, Changzhou Second People's Hospital Affiliated to Nanjing Medical University, Changzhou, Jiangsu 213000, P.R. China
    Copyright: © Xin et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 2047-2056
    |
    Published online on: October 19, 2020
       https://doi.org/10.3892/ijmm.2020.4761
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Abstract

Thyroid cancer (TC) is one of the most common malignancies with a high mortality rate. Long non‑coding RNA CCAT2 (CCAT2) participates in the occurrence and development of certain human cancers; however, whether it is involved in TC remains unclear. Thus, the present study investigated the role of CCAT2 in TC and the underlying mechanism. CCAT2 expression in both TC tissues and cell lines was examined by reverse transcription‑quantitative PCR. CCAT2 expression was silenced in TC cell lines by a specific small interfering (si)RNA against CCAT2 (si‑CCAT2). The effects of CCAT2 silencing on TC cell proliferation were detected by CCK‑8 and colony formation assays. Cell cycle and apoptosis of the treated TC cells were assessed by flow cytometry. Wound healing and Transwell assays were performed to detect the effects of si‑CCAT2 on the migration and invasion of TC cells. Apoptosis‑related proteins and Wnt/β‑catenin cascade‑associated agents were examined by western blotting. The interaction between CCAT2 and the Wnt/β‑catenin pathway in the transfected cells was detected by performing a dual‑luciferase reporter assay. CCAT2 expression was increased in TC tissue samples and cell lines compared with the controls. Tissue CCAT2 level was associated with T stage and tumor‑node‑metastasis stage of TC. Silencing CCAT2 inhibited TC cell proliferation, migration and invasion, and promoted TC cell cycle arrest and apoptosis. Furthermore, CCAT2 knockdown suppressed the activity of the Wnt/β‑catenin cascade in TC cells treated with lithium chloride. In summary, the present study demonstrated that CCAT2 knockdown suppresses TC progression via inactivating the Wnt/β‑catenin cascade, indicating that suppressing CCAT2 and the Wnt/β‑catenin signaling pathway may be a promising therapeutic strategy for treating TC.
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1 

Siegel RL, Miller KD and Jemal A: Cancer statistics, 2017. CA Cancer J Clin. 67:7–30. 2017. View Article : Google Scholar : PubMed/NCBI

2 

La Vecchia C, Malvezzi M, Bosetti C, Garavello W, Bertuccio P, Levi F and Negri E: Thyroid cancer mortality and incidence: A global overview. Int J Cancer. 136:2187–2195. 2015. View Article : Google Scholar

3 

Siegel RL, Miller KD and Jemal A: Cancer statistics, 2019. CA Cancer J Clin. 69:7–34. 2019. View Article : Google Scholar : PubMed/NCBI

4 

Yang L, Zheng RS, Wang N, Zeng HM, Yuan YN, Zhang SW, Li HC, Liu S, Chen WQ and He J: Analysis of incidence and mortality of thyroid cancer in China, 2013. Zhonghua Zhong Liu Za Zhi. 39:862–867. 2017.In Chinese. PubMed/NCBI

5 

Baloch ZW and LiVolsi VA: Special types of thyroid carcinoma. Histopathology. 72:40–52. 2018. View Article : Google Scholar

6 

Azar FK, Lee SL and Rosen JE: Medullary thyroid cancer: An update for surgeons. Am Surg. 81:1–8. 2015. View Article : Google Scholar : PubMed/NCBI

7 

Massimino M, Evans DB, Podda M, Spinelli C, Collini P, Pizzi N and Bleyer A: Thyroid cancer in adolescents and young adults. Pediatr Blood Cancer. 65:e270252018. View Article : Google Scholar : PubMed/NCBI

8 

Raue F and Frank-Raue K: Thyroid cancer: Risk-stratified management and individualized therapy. Clin Cancer Res. 22:5012–5021. 2016. View Article : Google Scholar : PubMed/NCBI

9 

Liu S, Liu X, Li J, Zhou H, Carr MJ, Zhang Z and Shi W: Long noncoding RNAs: Novel regulators of virus-host interactions. Rev Med Virol. 29:e20462019. View Article : Google Scholar : PubMed/NCBI

10 

Liz J and Esteller M: lncRNAs and microRNAs with a role in cancer development. Biochim Biophys Acta. 1859:169–176. 2016. View Article : Google Scholar

11 

Sui F, Ji M and Hou P: Long non-coding RNAs in thyroid cancer: Biological functions and clinical significance. Mol Cell Endocrinol. 469:11–22. 2018. View Article : Google Scholar

12 

Gugnoni M and Ciarrocchi A: Long noncoding RNA and epithelial mesenchymal transition in cancer. Int J Mol Sci. 20:19242019. View Article : Google Scholar :

13 

Lee KT, Gopalan V and Lam AK: Roles of long-non-coding RNAs in cancer therapy through the PI3K/Akt signalling pathway. Histol Histopathol. 34:593–609. 2019.PubMed/NCBI

14 

Fu D, Zhang Y and Cui H: Long noncoding RNA CCAT2 is activated by E2F1 and exerts oncogenic properties by interacting with PTTG1 in pituitary adenomas. Am J Cancer Res. 8:245–255. 2018.PubMed/NCBI

15 

Ruan R and Zhao XL: LncRNA CCAT2 enhances cell proliferation via GSK3β/β-catenin signaling pathway in human osteosarcoma. Eur Rev Med Pharmacol Sci. 22:2978–2984. 2018.PubMed/NCBI

16 

Fosselteder J, Calin GA and Pichler M: Long non-coding RNA CCAT2 as a therapeutic target in colorectal cancer. Expert Opin Ther Targets. 22:973–976. 2018. View Article : Google Scholar : PubMed/NCBI

17 

Miao S, Jing M, Sheng R, Cui D, Lu S, Zhang X, Jing S, Zhang X, Shan T, Shan H, et al: The analysis of differential diagnosis of benign and malignant thyroid nodules based on ultrasound reports. Gland surgery. 9:653–660. 2020. View Article : Google Scholar : PubMed/NCBI

18 

Lee H, Lee M, Byun SS, Lee SE and Hong SK: Evaluation of prostate cancer stage groups updated in the 8th edition of the American Joint Committee on cancer tumor-node-metastasis staging manual. Clin Genitourin Cancer. 17:e221–e226. 2019. View Article : Google Scholar

19 

Livak KJ and Schmittgen TD: Analysis of relative gene expression data using real-time quantitative PCR and the 2(-Delta Delta C(T)) method. Methods. 25:402–408. 2001. View Article : Google Scholar

20 

Srivastava NS and Srivastava RAK: Curcumin and quercetin synergistically inhibit cancer cell proliferation in multiple cancer cells and modulate Wnt/β-catenin signaling and apoptotic path-ways in A375 cells. Phytomedicine. 52:117–128. 2019. View Article : Google Scholar : PubMed/NCBI

21 

Zhao C, Qiao C, Zong L and Chen Y: Long non-coding RNA-CCAT2 promotes the occurrence of non-small cell lung cancer by regulating the Wnt/β-catenin signaling pathway. Oncol Lett. 16:4600–4606. 2018.PubMed/NCBI

22 

Ma Y, Hu X, Shang C, Zhong M and Guo Y: Silencing of long non-coding RNA CCAT2 depressed malignancy of oral squamous cell carcinoma via Wnt/β-catenin pathway. Tumour Biol. 39:10104283177176702017. View Article : Google Scholar

23 

Murugan AK, Munirajan AK and Alzahrani AS: Long noncoding RNAs: Emerging players in thyroid cancer pathogenesis. Endocr Relat Cancer. 25:R59–R82. 2018. View Article : Google Scholar

24 

Zhang R, Hardin H, Chen J, Guo Z and Lloyd RV: Non-coding RNAs in thyroid cancer. Endocr Pathol. 27:12–20. 2016. View Article : Google Scholar : PubMed/NCBI

25 

Xin Y, Li Z, Zheng H, Chan MTV and Ka Kei Wu W: CCAT2: A novel oncogenic long non-coding RNA in human cancers. Cell Prolif. 50:e123422017. View Article : Google Scholar

26 

Ling H, Spizzo R, Atlasi Y, Nicoloso M, Shimizu M, Redis RS, Nishida N, Gafà R, Song J, Guo Z, et al: CCAT2, a novel noncoding RNA mapping to 8q24, underlies metastatic progression and chromosomal instability in colon cancer. Genome Res. 23:1446–1461. 2013. View Article : Google Scholar : PubMed/NCBI

27 

Jo S, Ha TK, Han SH, Kim ME, Jung I, Lee HW, Bae SK and Lee JS: Myricetin induces apoptosis of human anaplastic thyroid cancer cells via mitochondria dysfunction. Anticancer Res. 37:1705–1710. 2017. View Article : Google Scholar : PubMed/NCBI

28 

Tirapelli DPDC, Lustosa IL, Menezes SB, Franco IM, Rodrigues AR, Peria FM, Marinho AMDN, Serafini LN, Carlotti CG Jr and Tirapelli LF: High expression of XIAP and Bcl-2 may inhibit programmed cell death in glioblastomas. Arq Neuropsiquiatr. 75:875–880. 2017. View Article : Google Scholar : PubMed/NCBI

29 

Warren CFA, Wong-Brown MW and Bowden NA: BCL-2 family isoforms in apoptosis and cancer. Cell Death Dis. 10:1772019. View Article : Google Scholar : PubMed/NCBI

30 

Hamivand Z, Haddadi G and Fardid R: Expression of Bax and Bcl2 genes in peripheral blood lymphocytes of patients with differentiated thyroid cancer. J Med Phys. 43:41–45. 2018.PubMed/NCBI

31 

Albasri AM, Elkablawy MA, Ansari IA and Alhujaily AS: Prognostic significance of cyclin D1 Over-expression in colorectal cancer: An experience from Madinah, Saudi Arabia. Asian Pac J Cancer Prev. 20:2471–2476. 2019. View Article : Google Scholar : PubMed/NCBI

32 

Nusse R and Clevers H: Wnt/β-catenin signaling, disease, and emerging therapeutic modalities. Cell. 169:985–999. 2017. View Article : Google Scholar : PubMed/NCBI

33 

Shang S, Hua F and Hu ZW: The regulation of β-catenin activity and function in cancer: Therapeutic opportunities. Oncotarget. 8:33972–33989. 2017. View Article : Google Scholar : PubMed/NCBI

34 

Krishnamurthy N and Kurzrock R: Targeting the Wnt/beta-catenin pathway in cancer: Update on effectors and inhibitors. Cancer Treat Rev. 62:50–60. 2018. View Article : Google Scholar

35 

Bi W, Huang J, Nie C, Liu B, He G, Han J, Pang R, Ding Z, Xu J and Zhang J: CircRNA circRNA_102171 promotes papillary thyroid cancer progression through modulating CTNNBIP1-dependent activation of β-catenin pathway. J Exp Clin Cancer Res. 37:2752018. View Article : Google Scholar

36 

Jing N, Huang T, Guo H, Yang J, Li M, Chen Z and Zhang Y: LncRNA CASC15 promotes colon cancer cell proliferation and metastasis by regulating the miR4310/LGR5/Wnt/β-catenin signaling pathway. Mol Med Rep. 18:2269–2276. 2018.PubMed/NCBI

37 

Zarkou V, Galaras A, Giakountis A and Hatzis P: Crosstalk mechanisms between the WNT signaling pathway and long non-coding RNAs. Noncoding RNA Res. 3:42–53. 2018. View Article : Google Scholar : PubMed/NCBI

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Copy and paste a formatted citation
Spandidos Publications style
Xin S and Ye X: Knockdown of long non‑coding RNA CCAT2 suppresses the progression of thyroid cancer by inhibiting the Wnt/β‑catenin pathway. Int J Mol Med 46: 2047-2056, 2020.
APA
Xin, S., & Ye, X. (2020). Knockdown of long non‑coding RNA CCAT2 suppresses the progression of thyroid cancer by inhibiting the Wnt/β‑catenin pathway. International Journal of Molecular Medicine, 46, 2047-2056. https://doi.org/10.3892/ijmm.2020.4761
MLA
Xin, S., Ye, X."Knockdown of long non‑coding RNA CCAT2 suppresses the progression of thyroid cancer by inhibiting the Wnt/β‑catenin pathway". International Journal of Molecular Medicine 46.6 (2020): 2047-2056.
Chicago
Xin, S., Ye, X."Knockdown of long non‑coding RNA CCAT2 suppresses the progression of thyroid cancer by inhibiting the Wnt/β‑catenin pathway". International Journal of Molecular Medicine 46, no. 6 (2020): 2047-2056. https://doi.org/10.3892/ijmm.2020.4761
Copy and paste a formatted citation
x
Spandidos Publications style
Xin S and Ye X: Knockdown of long non‑coding RNA CCAT2 suppresses the progression of thyroid cancer by inhibiting the Wnt/β‑catenin pathway. Int J Mol Med 46: 2047-2056, 2020.
APA
Xin, S., & Ye, X. (2020). Knockdown of long non‑coding RNA CCAT2 suppresses the progression of thyroid cancer by inhibiting the Wnt/β‑catenin pathway. International Journal of Molecular Medicine, 46, 2047-2056. https://doi.org/10.3892/ijmm.2020.4761
MLA
Xin, S., Ye, X."Knockdown of long non‑coding RNA CCAT2 suppresses the progression of thyroid cancer by inhibiting the Wnt/β‑catenin pathway". International Journal of Molecular Medicine 46.6 (2020): 2047-2056.
Chicago
Xin, S., Ye, X."Knockdown of long non‑coding RNA CCAT2 suppresses the progression of thyroid cancer by inhibiting the Wnt/β‑catenin pathway". International Journal of Molecular Medicine 46, no. 6 (2020): 2047-2056. https://doi.org/10.3892/ijmm.2020.4761
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