Knockdown of STIP1 inhibits the invasion of CD133‑positive cancer stem‑like cells of the osteosarcoma MG63 cell line via the PI3K/Akt and ERK1/2 pathways

  • Authors:
    • Jian‑Hua Wang
    • Chen Gong
    • Feng‑Jin Guo
    • Xiao Zhou
    • Ming‑Sheng Zhang
    • Hong Qiu
    • Teng‑Fei Chao
    • Yang Liu
    • Liang Qin
    • Hui‑Hua Xiong
  • View Affiliations

  • Published online on: October 21, 2020     https://doi.org/10.3892/ijmm.2020.4764
  • Pages: 2251-2259
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Abstract

Osteosarcoma is the most common primary malignant tumor of the bone in adolescents and children, with high rates of metastasis and a poor prognosis. Recently, osteosarcoma cancer stem/stem‑like cells (CSCs) have been identified as the main cause of recurrence and metastasis. Stress‑induced phosphoprotein 1 (STIP1), a co‑chaperone that binds to heat shock proteins 70 and 90, is abnormally expressed in several tumor cell lines, and may play an important role in tumor cell migration and invasion. These features indicate that STIP1 may represent a new therapeutic target for osteosarcoma CSCs. However, the role of STIP1 in osteosarcoma CSC migration and invasion remains largely unknown. In the present study, CD133‑positive osteosarcoma CSCs were first isolated and cultured by magnetic cell sorting and serum‑free medium suspension cell sphere culture, respectively. Knockdown of STIP1 by small interfering RNA significantly was then shown to inhibit the migration and invasion of these cells, possibly due to the regulation of the expression of matrix metalloproteinase (MMP)‑2, MMP‑9 and tissue inhibitor of metalloproteinase‑2. Furthermore, data from the present study suggested that the knockdown of STIP1 decreased the levels of phosphorylated Akt and phosphorylated ERK1/2. In summary, these findings indicate that targeting STIP1 in osteosarcoma may constitute a viable molecular targeted therapy strategy for the inhibition of CSC invasion and migration.
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December-2020
Volume 46 Issue 6

Print ISSN: 1107-3756
Online ISSN:1791-244X

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Spandidos Publications style
Wang JH, Gong C, Guo FJ, Zhou X, Zhang MS, Qiu H, Chao TF, Liu Y, Qin L, Xiong HH, Xiong HH, et al: Knockdown of STIP1 inhibits the invasion of CD133‑positive cancer stem‑like cells of the osteosarcoma MG63 cell line via the PI3K/Akt and ERK1/2 pathways. Int J Mol Med 46: 2251-2259, 2020
APA
Wang, J., Gong, C., Guo, F., Zhou, X., Zhang, M., Qiu, H. ... Xiong, H. (2020). Knockdown of STIP1 inhibits the invasion of CD133‑positive cancer stem‑like cells of the osteosarcoma MG63 cell line via the PI3K/Akt and ERK1/2 pathways. International Journal of Molecular Medicine, 46, 2251-2259. https://doi.org/10.3892/ijmm.2020.4764
MLA
Wang, J., Gong, C., Guo, F., Zhou, X., Zhang, M., Qiu, H., Chao, T., Liu, Y., Qin, L., Xiong, H."Knockdown of STIP1 inhibits the invasion of CD133‑positive cancer stem‑like cells of the osteosarcoma MG63 cell line via the PI3K/Akt and ERK1/2 pathways". International Journal of Molecular Medicine 46.6 (2020): 2251-2259.
Chicago
Wang, J., Gong, C., Guo, F., Zhou, X., Zhang, M., Qiu, H., Chao, T., Liu, Y., Qin, L., Xiong, H."Knockdown of STIP1 inhibits the invasion of CD133‑positive cancer stem‑like cells of the osteosarcoma MG63 cell line via the PI3K/Akt and ERK1/2 pathways". International Journal of Molecular Medicine 46, no. 6 (2020): 2251-2259. https://doi.org/10.3892/ijmm.2020.4764