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Bleomycin A5 suppresses Drp1‑mediated mitochondrial fission and induces apoptosis in human nasal polyp‑derived fibroblasts

  • Authors:
    • Fan Wu
    • Yun Ma
    • Jingyi Wang
    • Huashuang Ou
    • Hua Dang
    • Yiqing Zheng
    • Peng Tian
    • Hua Zou
  • View Affiliations / Copyright

    Affiliations: Department of Otorhinolaryngology‑Head and Neck Surgery, Sun Yat‑sen Memorial Hospital, Sun Yat‑sen University, Guangzhou, Guangdong 510000, P.R. China
    Copyright: © Wu et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 346-360
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    Published online on: November 23, 2020
       https://doi.org/10.3892/ijmm.2020.4797
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Abstract

Intralesional injection of bleomycin‑A5 (BLE‑A5) is a novel treatment for nasal polyps. Our previous study clarified that BLE‑A5 could induce nasal polyp‑derived fibroblast (NPDF) apoptosis in nasal polyps. However, the detailed mechanisms are still unclear. The present study aimed to determine the effects of BLE‑A5 on NPDF mitochondrial dynamics and provide a theoretical basis for the local application of BLE‑A5 to treat nasal polyps. In the present study, an in vitro nasal polyp tissue culture model was used to define the BLE‑A5 target cell type in nasal polyps. NPDF primary cell culture was used to study the effects of BLE‑A5 on the mitochondrial dynamic‑related mechanism. The results showed that BLE‑A5 treatment of NPDFs caused mitochondrial‑mediated apoptosis. Dynamin‑related protein 1 (Drp1) was shown to be altered in BLE‑A5‑treated NPDFs. Drp1 knockdown increased the sensitivity of NPDFs to BLE‑A5 and exacerbated mitochondrial dysfunction. BLE‑A5 decreased cyclin B1‑CDK1 complex‑mediated phosphorylation of Drp1 and inhibited Drp1‑mediated mitophagy in NPDFs. Overall, the present study concluded that BLE‑A5 mainly induces NPDF apoptosis in nasal polyps. BLE‑A5 regulates the mitochondria by inhibiting Drp1 activation, resulting in NPDF mitochondrial dynamic disorder and apoptosis.
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Wu F, Ma Y, Wang J, Ou H, Dang H, Zheng Y, Tian P and Zou H: Bleomycin A5 suppresses Drp1‑mediated mitochondrial fission and induces apoptosis in human nasal polyp‑derived fibroblasts. Int J Mol Med 47: 346-360, 2021.
APA
Wu, F., Ma, Y., Wang, J., Ou, H., Dang, H., Zheng, Y. ... Zou, H. (2021). Bleomycin A5 suppresses Drp1‑mediated mitochondrial fission and induces apoptosis in human nasal polyp‑derived fibroblasts. International Journal of Molecular Medicine, 47, 346-360. https://doi.org/10.3892/ijmm.2020.4797
MLA
Wu, F., Ma, Y., Wang, J., Ou, H., Dang, H., Zheng, Y., Tian, P., Zou, H."Bleomycin A5 suppresses Drp1‑mediated mitochondrial fission and induces apoptosis in human nasal polyp‑derived fibroblasts". International Journal of Molecular Medicine 47.1 (2021): 346-360.
Chicago
Wu, F., Ma, Y., Wang, J., Ou, H., Dang, H., Zheng, Y., Tian, P., Zou, H."Bleomycin A5 suppresses Drp1‑mediated mitochondrial fission and induces apoptosis in human nasal polyp‑derived fibroblasts". International Journal of Molecular Medicine 47, no. 1 (2021): 346-360. https://doi.org/10.3892/ijmm.2020.4797
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Spandidos Publications style
Wu F, Ma Y, Wang J, Ou H, Dang H, Zheng Y, Tian P and Zou H: Bleomycin A5 suppresses Drp1‑mediated mitochondrial fission and induces apoptosis in human nasal polyp‑derived fibroblasts. Int J Mol Med 47: 346-360, 2021.
APA
Wu, F., Ma, Y., Wang, J., Ou, H., Dang, H., Zheng, Y. ... Zou, H. (2021). Bleomycin A5 suppresses Drp1‑mediated mitochondrial fission and induces apoptosis in human nasal polyp‑derived fibroblasts. International Journal of Molecular Medicine, 47, 346-360. https://doi.org/10.3892/ijmm.2020.4797
MLA
Wu, F., Ma, Y., Wang, J., Ou, H., Dang, H., Zheng, Y., Tian, P., Zou, H."Bleomycin A5 suppresses Drp1‑mediated mitochondrial fission and induces apoptosis in human nasal polyp‑derived fibroblasts". International Journal of Molecular Medicine 47.1 (2021): 346-360.
Chicago
Wu, F., Ma, Y., Wang, J., Ou, H., Dang, H., Zheng, Y., Tian, P., Zou, H."Bleomycin A5 suppresses Drp1‑mediated mitochondrial fission and induces apoptosis in human nasal polyp‑derived fibroblasts". International Journal of Molecular Medicine 47, no. 1 (2021): 346-360. https://doi.org/10.3892/ijmm.2020.4797
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