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CircUSP36 knockdown alleviates oxidized low‑density lipoprotein‑induced cell injury and inflammatory responses in human umbilical vein endothelial cells via the miR‑20a‑5p/ROCK2 axis

  • Authors:
    • Jun Miao
    • Bo Wang
    • Ran Shao
    • Yan Wang
  • View Affiliations / Copyright

    Affiliations: Department of Cardiology, Zaozhuang Municipal Hospital, Zaozhuang, Shandong 277100, P.R. China, Department of Cardiology, Zaozhuang Hospital of Zaozhuang Mining Group, Zaozhuang, Shandong 277100, P.R. China, Nursing Department, Zaozhuang Hospital of Traditional Chinese Medicine, Zaozhuang, Shandong 277100, P.R. China
    Copyright: © Miao et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Article Number: 40
    |
    Published online on: February 5, 2021
       https://doi.org/10.3892/ijmm.2021.4873
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Abstract

The dysfunctions of human umbilical vein endothelial cells (HUVECs) are important features of atherosclerosis (AS). Circular RNAs (circRNAs) are regulators of a wide range of human diseases, including AS. The present study aimed to investigate the role of circUSP36 in the ectopic phenotype of HUVECs and to provide evidence of the involvement of circUSP36 in the pathogenesis of AS. AS cell models in vitro were established using HUVECs exposed to oxidized low‑density lipoprotein (ox‑LDL). Cell viability, cell cycle progression and apoptosis, and cell migration and invasion were assessed by cell counting kit‑8 (CCK‑8) assay, flow cytometric assay and Transwell assay, respectively. The expression levels or releases of pro‑inflammatory factors were detected by western blot analysis or enzyme‑linked immunosorbent assay (ELISA). The mRNA expression of circUSP36, miR‑20a‑5p and Rho‑associated coiled‑coil kinase 2 (ROCK2) was detected by reverse transcription‑quantitative polymerase chain reaction (RT‑qPCR), and the protein level of ROCK2 was detected by western blot analysis. The targeted association between miR‑20a‑5p and circUSP36 or ROCK2 was verified by dual‑luciferase reporter assay and RNA immunoprecipitation (RIP) assay. The results revealed that circUSP36 was highly expressed in ox‑LDL‑exposed HUVECs. CircUSP36 knockdown attenuated ox‑LDL‑induced cell cycle arrest, cell apoptosis and inflammatory responses, and promoted cell migration and invasion which had been blocked by ox‑LDL. miR‑20a‑5p was found to be a target of circUSP36, and miR‑20a‑5p inhibition reversed the effects of circUSP36 knockdown. Moreover, miR‑20a‑5p directly bound to ROCK2, and miR‑20a‑5p inhibition aggravated ox‑LDL‑induced injury by increasing the ROCK2 level. More importantly, circUSP36 targeted miR‑20a‑5p to regulate the expression of ROCK2. On the whole, the present study demonstrates that, circUSP36 regulates ox‑LDL‑induced HUVEC injury and inflammation by modulating ROCK2 via competitively targeting miR‑20a‑5p.
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1 

Libby P and Hansson GK: Inflammation and immunity in diseases of the arterial tree: Players and layers. Circ Res. 116:307–311. 2015. View Article : Google Scholar : PubMed/NCBI

2 

Li B, Li W, Li X and Zhou H: Inflammation: A novel therapeutic target/direction in atherosclerosis. Curr Pharm Des. 23:1216–1227. 2017. View Article : Google Scholar

3 

Gimbrone MA Jr and García-Cardeña G: Endothelial cell dysfunction and the pathobiology of atherosclerosis. Circ Res. 118:620–636. 2016. View Article : Google Scholar : PubMed/NCBI

4 

Wang J, Wang Y, Li Y, Wang S and Zhu X: Platelet-rich plasma protects HUVECs against oX-LDL-induced injury. Open Med (Wars). 13:41–52. 2018. View Article : Google Scholar

5 

Kattoor AJ, Goel A and Mehta JL: LOX-1: Regulation, signaling and its role in atherosclerosis. Antioxidants (Basel). 8:2182019. View Article : Google Scholar

6 

Mitra S, Deshmukh A, Sachdeva R, Lu J and Mehta JL: Oxidized low-density lipoprotein and atherosclerosis implications in anti-oxidant therapy. Am J Med Sci. 342:135–142. 2011. View Article : Google Scholar : PubMed/NCBI

7 

Yin J, Hou X and Yang S: microRNA-338-3p promotes ox-LDL-induced endothelial cell injury through targeting BAMBI and activating TGF-β/Smad pathway. J Cell Physiol. 234:11577–11586. 2019. View Article : Google Scholar

8 

Peng J, Tang ZH, Ren Z, He B, Zeng Y, Liu LS, Wang Z, Wei DH, Zheng XL and Jiang ZS: TET2 protects against oxLDL-Induced HUVEC dysfunction by upregulating the CSE/H2S system. Front Pharmacol. 8:4862017. View Article : Google Scholar :

9 

Zeng X, Lin W, Guo M and Zou Q: A comprehensive overview and evaluation of circular RNA detection tools. PLoS Comput Biol. 13:e10054202017. View Article : Google Scholar : PubMed/NCBI

10 

Chen I, Chen CY and Chuang TJ: Biogenesis, identification, and function of exonic circular RNAs. Wiley Interdiscip Rev RNA. 6:563–579. 2015. View Article : Google Scholar : PubMed/NCBI

11 

Bayoumi AS, Aonuma T, Teoh JP, Tang YL and Kim IM: Circular noncoding RNAs as potential therapies and circulating biomarkers for cardiovascular diseases. Acta Pharmacol Sin. 39:1100–1109. 2018. View Article : Google Scholar : PubMed/NCBI

12 

Pan RY, Zhao CH, Yuan JX, Zhang YJ, Jin JL, Gu MF, Mao ZY, Sun HJ, Jia QW, Ji MY, et al: Circular RNA profile in coronary artery disease. Am J Transl Res. 11:7115–7125. 2019.PubMed/NCBI

13 

Li CY, Ma L and Yu B: Circular RNA hsa_circ_0003575 regulates oxLDL induced vascular endothelial cells proliferation and angiogenesis. Biomed Pharmacother. 95:1514–1519. 2017. View Article : Google Scholar : PubMed/NCBI

14 

Feinberg MW and Moore KJ: MicroRNA regulation of atherosclerosis. Circ Res. 118:703–720. 2016. View Article : Google Scholar : PubMed/NCBI

15 

Dlouhá D and Hubáček JA: Regulatory RNAs and cardiovascular disease- with a special focus on circulating microRNAs. Physiol Res. 66(Suppl 1): S21–S38. 2017. View Article : Google Scholar

16 

Wojciechowska A, Braniewska A and Kozar-Kamińska K: MicroRNA in cardiovascular biology and disease. Adv Clin Exp Med. 26:865–874. 2017. View Article : Google Scholar : PubMed/NCBI

17 

Gao G, Chen W, Liu M, Yan X and Yang P: Circulating MicroRNAs as novel potential biomarkers for left ventricular remodeling in postinfarction heart failure. Dis Markers. 2019:50938032019. View Article : Google Scholar : PubMed/NCBI

18 

Muendlein A, Geiger K, Leiherer A, Saely CH, Fraunberger P and Drexel H: Evaluation of the associations between circulating microRNAs and kidney function in coronary angiography patients. Am J Physiol Renal Physiol. 318:F315–F321. 2020. View Article : Google Scholar

19 

Shahbazi R, Baradaran B, Khordadmehr M, Safaei S, Baghbanzadeh A, Jigari F and Ezzati H: Targeting ROCK signaling in health, malignant and non-malignant diseases. Immunol Lett. 219:15–26. 2020. View Article : Google Scholar : PubMed/NCBI

20 

Okamoto R, Li Y, Noma K, Hiroi Y, Liu PY, Taniguchi M, Ito M and Liao JK: FHL2 prevents cardiac hypertrophy in mice with cardiac-specific deletion of ROCK2. FASEB J. 27:1439–1449. 2013. View Article : Google Scholar :

21 

Livak KJ and Schmittgen TD: Analysis of relative gene expression data using real-time quantitative PCR and the 2(-Delta Delta C(T)) method. Methods. 25:402–408. 2001. View Article : Google Scholar

22 

Singh RB, Mengi SA, Xu YJ, Arneja AS and Dhalla NS: Pathogenesis of atherosclerosis: A multifactorial process. Exp Clin Cardiol. 7:40–53. 2002.PubMed/NCBI

23 

Yang L, Yang F, Zhao H, Wang M and Zhang Y: Circular RNA circCHFR facilitates the proliferation and migration of vascular smooth muscle via miR-370/FOXO1/Cyclin D1 pathway. Mol Ther Nucleic Acids. 16:434–441. 2019. View Article : Google Scholar : PubMed/NCBI

24 

Song CL, Wang JP, Xue X, Liu N, Zhang XH, Zhao Z, Liu JG, Zhang CP, Piao ZH, Liu Y and Yang YB: Effect of circular ANRIL on the inflammatory response of vascular endothelial cells in a rat model of coronary atherosclerosis. Cell Physiol Biochem. 42:1202–1212. 2017. View Article : Google Scholar : PubMed/NCBI

25 

Zhang S, Song G, Yuan J, Qiao S, Xu S, Si Z, Yang Y, Xu X and Wang A: Circular RNA circ_0003204 inhibits proliferation, migration and tube formation of endothelial cell in atherosclerosis via miR-370-3p/TGFbetaR2/phosph-SMAD3 axis. J Biomed Sci. 27:112020. View Article : Google Scholar

26 

Liu H, Ma X, Mao Z, Shen M, Zhu J and Chen F: Circular RNA has_circ_0003204 inhibits oxLDL-induced vascular endothelial cell proliferation and angiogenesis. Cell Signal. 70:1095952020. View Article : Google Scholar : PubMed/NCBI

27 

Memczak S, Jens M, Elefsinioti A, Torti F, Krueger J, Rybak A, Maier L, Mackowiak SD, Gregersen LH, Munschauer M, et al: Circular RNAs are a large class of animal RNAs with regulatory potency. Nature. 495:333–338. 2013. View Article : Google Scholar : PubMed/NCBI

28 

Chen M, Li W, Zhang Y and Yang J: MicroRNA-20a protects human aortic endothelial cells from Ox-LDL-induced inflammation through targeting TLR4 and TXNIP signaling. Biomed Pharmacother. 103:191–197. 2018. View Article : Google Scholar : PubMed/NCBI

29 

Wang D, Wang Y, Ma J, Wang W, Sun B, Zheng T, Wei M and Sun Y: MicroRNA-20a participates in the aerobic exercise-based prevention of coronary artery disease by targeting PTEN. Biomed Pharmacother. 95:756–763. 2017. View Article : Google Scholar : PubMed/NCBI

30 

Zhou Q, Mei Y, Shoji T, Han X, Kaminski K, Oh GT, Ongusaha PP, Zhang K, Schmitt H, Moser M, et al: Rho-associated coiled-coil-containing kinase 2 deficiency in bone marrow-derived cells leads to increased cholesterol efflux and decreased atherosclerosis. Circulation. 126:2236–2247. 2012. View Article : Google Scholar : PubMed/NCBI

31 

Mattaliano MD, Wooters J, Shih HH and Paulsen JE: ROCK2 associates with lectin-like oxidized LDL receptor-1 and mediates oxidized LDL-induced IL-8 production. Am J Physiol Cell Physiol. 298:C1180–1187. 2010. View Article : Google Scholar : PubMed/NCBI

32 

Huang L, Dai F, Tang L, Bao X, Liu Z, Huang C, Zhang T and Yao W: Distinct roles for ROCK1 and ROCK2 in the regulation of oxldl-mediated endothelial dysfunction. Cell Physiol Biochem. 49:565–577. 2018. View Article : Google Scholar : PubMed/NCBI

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Copy and paste a formatted citation
Spandidos Publications style
Miao J, Wang B, Shao R and Wang Y: CircUSP36 knockdown alleviates oxidized low‑density lipoprotein‑induced cell injury and inflammatory responses in human umbilical vein endothelial cells via the miR‑20a‑5p/ROCK2 axis. Int J Mol Med 47: 40, 2021.
APA
Miao, J., Wang, B., Shao, R., & Wang, Y. (2021). CircUSP36 knockdown alleviates oxidized low‑density lipoprotein‑induced cell injury and inflammatory responses in human umbilical vein endothelial cells via the miR‑20a‑5p/ROCK2 axis. International Journal of Molecular Medicine, 47, 40. https://doi.org/10.3892/ijmm.2021.4873
MLA
Miao, J., Wang, B., Shao, R., Wang, Y."CircUSP36 knockdown alleviates oxidized low‑density lipoprotein‑induced cell injury and inflammatory responses in human umbilical vein endothelial cells via the miR‑20a‑5p/ROCK2 axis". International Journal of Molecular Medicine 47.4 (2021): 40.
Chicago
Miao, J., Wang, B., Shao, R., Wang, Y."CircUSP36 knockdown alleviates oxidized low‑density lipoprotein‑induced cell injury and inflammatory responses in human umbilical vein endothelial cells via the miR‑20a‑5p/ROCK2 axis". International Journal of Molecular Medicine 47, no. 4 (2021): 40. https://doi.org/10.3892/ijmm.2021.4873
Copy and paste a formatted citation
x
Spandidos Publications style
Miao J, Wang B, Shao R and Wang Y: CircUSP36 knockdown alleviates oxidized low‑density lipoprotein‑induced cell injury and inflammatory responses in human umbilical vein endothelial cells via the miR‑20a‑5p/ROCK2 axis. Int J Mol Med 47: 40, 2021.
APA
Miao, J., Wang, B., Shao, R., & Wang, Y. (2021). CircUSP36 knockdown alleviates oxidized low‑density lipoprotein‑induced cell injury and inflammatory responses in human umbilical vein endothelial cells via the miR‑20a‑5p/ROCK2 axis. International Journal of Molecular Medicine, 47, 40. https://doi.org/10.3892/ijmm.2021.4873
MLA
Miao, J., Wang, B., Shao, R., Wang, Y."CircUSP36 knockdown alleviates oxidized low‑density lipoprotein‑induced cell injury and inflammatory responses in human umbilical vein endothelial cells via the miR‑20a‑5p/ROCK2 axis". International Journal of Molecular Medicine 47.4 (2021): 40.
Chicago
Miao, J., Wang, B., Shao, R., Wang, Y."CircUSP36 knockdown alleviates oxidized low‑density lipoprotein‑induced cell injury and inflammatory responses in human umbilical vein endothelial cells via the miR‑20a‑5p/ROCK2 axis". International Journal of Molecular Medicine 47, no. 4 (2021): 40. https://doi.org/10.3892/ijmm.2021.4873
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