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Helichrysetin and TNF‑α synergistically promote apoptosis by inhibiting overactivation of the NF‑κB and EGFR signaling pathways in HeLa and T98G cells

  • Authors:
    • Zhiying Wang
    • Xiaohui Liang
    • Aizhen Xiong
    • Lili Ding
    • Wei Li
    • Li Yang
    • Xiaojun Wu
    • Hailian Shi
    • Yue Zhou
    • Zhengtao Wang

  • View Affiliations

  • Published online on: February 10, 2021     https://doi.org/10.3892/ijmm.2021.4882
  • Article Number: 49

  • Copyright: © Wang et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

Tumor necrosis factor‑α (TNF‑α) has different effects on apoptosis depending on activation or inactivation of the nuclear factor‑κB (NF‑κB) and epidermal growth factor receptor (EGFR) signaling pathways. Helichrysetin, a natural chalcone, inhibits NF‑κB nuclear translocation in mouse pancreatic β cells. The present study aimed to identify the effect of helichrysetin on activation of the NF‑κB and EGFR signaling pathways induced by TNF‑α, and the synergistic effect of helichrysetin and TNF‑α on apoptosis of HeLa and T98G cells. Cell proliferation was measured by Cell Counting Kit‑8 assay, while apoptosis was measured by Hoechst 33258 and Annexin V/PI staining. NF‑κB activity was detected by luciferase assay, protein expression was measured by western blotting and mRNA expression was detected by quantitative PCR assay. The results revealed that in HeLa and T98G cells helichrysetin blocked the increased phosphorylation of NF‑κB p65 induced by TNF‑α. Although helichrysetin alone decreased cell viability, helichrysetin and TNF‑α synergistically decreased cell viability. Helichrysetin, not TNF‑α, promoted apoptosis, while the combination of helichrysetin and TNF‑α synergistically increased apoptosis. In addition, helichrysetin and TNF‑α synergistically enhanced the activation of caspase‑3 and poly‑(ADP‑ribose)‑polymerase compared with helichrysetin alone. Helichrysetin inhibited the phosphorylation of transforming growth factor‑β activated kinase (TAK1), IκB kinase‑α/β (IKK‑α/β), NF‑κB p65 and EGFR induced by TNF‑α. Consistent with the inhibition of NF‑κB activation, the increased TNF‑α‑induced mRNA expression levels of TNF‑α, IL‑1β, CCL2, CCL5 and CXCL10 were significantly downregulated by helichrysetin. Therefore, helichrysetin and TNF‑α synergistically promoted apoptosis by inhibiting TAK1/IKK/NF‑κB and TAK1/EGFR signaling pathways in HeLa and T98G cells, indicating a potential therapeutic strategy for cancer.
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April-2021
Volume 47 Issue 4

Print ISSN: 1107-3756
Online ISSN:1791-244X

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  • Impact Factor: 5.4
  • Ranked Medicine Research and Experimental
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  • CiteScore: 10.7
  • CiteScore Rank: Q1: #35/328 Biochemistry, Genetics and Molecular Biology
  • Source Normalized Impact per Paper (SNIP): 0.994
  • SCImago Journal Rank (SJR): 0.922
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Spandidos Publications style
Wang Z, Liang X, Xiong A, Ding L, Li W, Yang L, Wu X, Shi H, Zhou Y, Wang Z, Wang Z, et al: Helichrysetin and TNF‑α synergistically promote apoptosis by inhibiting overactivation of the NF‑κB and EGFR signaling pathways in HeLa and T98G cells. Int J Mol Med 47: 49, 2021
APA
Wang, Z., Liang, X., Xiong, A., Ding, L., Li, W., Yang, L. ... Wang, Z. (2021). Helichrysetin and TNF‑α synergistically promote apoptosis by inhibiting overactivation of the NF‑κB and EGFR signaling pathways in HeLa and T98G cells. International Journal of Molecular Medicine, 47, 49. https://doi.org/10.3892/ijmm.2021.4882
MLA
Wang, Z., Liang, X., Xiong, A., Ding, L., Li, W., Yang, L., Wu, X., Shi, H., Zhou, Y., Wang, Z."Helichrysetin and TNF‑α synergistically promote apoptosis by inhibiting overactivation of the NF‑κB and EGFR signaling pathways in HeLa and T98G cells". International Journal of Molecular Medicine 47.4 (2021): 49.
Chicago
Wang, Z., Liang, X., Xiong, A., Ding, L., Li, W., Yang, L., Wu, X., Shi, H., Zhou, Y., Wang, Z."Helichrysetin and TNF‑α synergistically promote apoptosis by inhibiting overactivation of the NF‑κB and EGFR signaling pathways in HeLa and T98G cells". International Journal of Molecular Medicine 47, no. 4 (2021): 49. https://doi.org/10.3892/ijmm.2021.4882