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Upregulated long non‑coding RNA LincIN promotes tumor progression via the regulation of nuclear factor 90/microRNA‑7/HOXB13 in esophageal squamous cell carcinoma

  • Authors:
    • Zhibo Tan
    • Peitao Zhou
    • Zhenru Zhu
    • Ying Wang
    • Zeqin Guo
    • Mengying Shen
    • Yazhi Xiao
    • Weixi Shen
    • Dehua Wu
  • View Affiliations / Copyright

    Affiliations: Department of Radiation Oncology, Nanfang Hospital, Southern Medical University, Guangzhou, Guangdong 510515, P.R. China, Department of Medical Oncology, National Cancer Centre/National Clinical Research Centre for Cancer/Cancer Hospital and Shenzhen Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College, Shenzhen, Guangdong 518116, P.R. China, Hepatology Unit and Department of Infectious Diseases, Nanfang Hospital, Southern Medical University, Guangzhou, Guangdong 510515, P.R. China, Department of Oncology, Shenzhen Hospital, Southern Medical University, Shenzhen, Guangdong 518100, P.R. China
    Copyright: © Tan et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Article Number: 78
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    Published online on: March 10, 2021
       https://doi.org/10.3892/ijmm.2021.4911
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Abstract

Long non‑coding RNA LincIN has been reported to be overexpressed and to be involved in the metastasis of breast cancer. However, the expression and role of LincIN in esophageal squamous cell carcinoma (ESCC) remain unsolved. In the present study, LincIN expression was examined in ESCC by RT‑qPCR, and the roles of LincIN in ESCC were determined using cell growth, migration and invasion assays. In addition, the effects of LincIN on nuclear factor 90 (NF90) and microRNA/miR (miR)‑7 were examined by RNA immunoprecipitation assay, RT‑qPCR, dual‑luciferase reporter assay and western blot analysis. The results revealed that LincIN expression was significantly increased in ESCC tissues and cell lines. The increased expression of LincIN was positively associated with invasion depth, lymph node metastasis, TNM stage and a poor prognosis. Functional assays revealed that the overexpression of LincIN promoted ESCC cell growth, migration and invasion. Mechanistic analysis revealed that LincIN physically bound to NF90, enhanced the binding between NF90 and primary miR‑7 (pri‑miR‑7), and further enhanced the inhibitory effects of NF90 on miR‑7 biogenesis. Therefore, LincIN downregulated miR‑7 expression in ESCC. The expression of miR‑7 inversely correlated with that of LincIN in ESCC tissues. By downregulating miR‑7, LincIN increased the expression of HOXB13, a target of miR‑7. The overexpression of miR‑7 or the depletion of HOXB13 both attenuated the tumor‑promoting roles of LincIN in ESCC cell growth, migration and invasion. On the whole, the findings of the present study suggest that LincIN is overexpressed and plays an oncogenic role in ESCC via the regulation of the NF90/miR‑7/HOXB13 axis. Thus, LincIN may prove to be a promising prognostic biomarker and therapeutic target for ESCC.
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Copy and paste a formatted citation
Spandidos Publications style
Tan Z, Zhou P, Zhu Z, Wang Y, Guo Z, Shen M, Xiao Y, Shen W and Wu D: Upregulated long non‑coding RNA LincIN promotes tumor progression via the regulation of nuclear factor 90/microRNA‑7/HOXB13 in esophageal squamous cell carcinoma. Int J Mol Med 47: 78, 2021.
APA
Tan, Z., Zhou, P., Zhu, Z., Wang, Y., Guo, Z., Shen, M. ... Wu, D. (2021). Upregulated long non‑coding RNA LincIN promotes tumor progression via the regulation of nuclear factor 90/microRNA‑7/HOXB13 in esophageal squamous cell carcinoma. International Journal of Molecular Medicine, 47, 78. https://doi.org/10.3892/ijmm.2021.4911
MLA
Tan, Z., Zhou, P., Zhu, Z., Wang, Y., Guo, Z., Shen, M., Xiao, Y., Shen, W., Wu, D."Upregulated long non‑coding RNA LincIN promotes tumor progression via the regulation of nuclear factor 90/microRNA‑7/HOXB13 in esophageal squamous cell carcinoma". International Journal of Molecular Medicine 47.5 (2021): 78.
Chicago
Tan, Z., Zhou, P., Zhu, Z., Wang, Y., Guo, Z., Shen, M., Xiao, Y., Shen, W., Wu, D."Upregulated long non‑coding RNA LincIN promotes tumor progression via the regulation of nuclear factor 90/microRNA‑7/HOXB13 in esophageal squamous cell carcinoma". International Journal of Molecular Medicine 47, no. 5 (2021): 78. https://doi.org/10.3892/ijmm.2021.4911
Copy and paste a formatted citation
x
Spandidos Publications style
Tan Z, Zhou P, Zhu Z, Wang Y, Guo Z, Shen M, Xiao Y, Shen W and Wu D: Upregulated long non‑coding RNA LincIN promotes tumor progression via the regulation of nuclear factor 90/microRNA‑7/HOXB13 in esophageal squamous cell carcinoma. Int J Mol Med 47: 78, 2021.
APA
Tan, Z., Zhou, P., Zhu, Z., Wang, Y., Guo, Z., Shen, M. ... Wu, D. (2021). Upregulated long non‑coding RNA LincIN promotes tumor progression via the regulation of nuclear factor 90/microRNA‑7/HOXB13 in esophageal squamous cell carcinoma. International Journal of Molecular Medicine, 47, 78. https://doi.org/10.3892/ijmm.2021.4911
MLA
Tan, Z., Zhou, P., Zhu, Z., Wang, Y., Guo, Z., Shen, M., Xiao, Y., Shen, W., Wu, D."Upregulated long non‑coding RNA LincIN promotes tumor progression via the regulation of nuclear factor 90/microRNA‑7/HOXB13 in esophageal squamous cell carcinoma". International Journal of Molecular Medicine 47.5 (2021): 78.
Chicago
Tan, Z., Zhou, P., Zhu, Z., Wang, Y., Guo, Z., Shen, M., Xiao, Y., Shen, W., Wu, D."Upregulated long non‑coding RNA LincIN promotes tumor progression via the regulation of nuclear factor 90/microRNA‑7/HOXB13 in esophageal squamous cell carcinoma". International Journal of Molecular Medicine 47, no. 5 (2021): 78. https://doi.org/10.3892/ijmm.2021.4911
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