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Knockdown of long non‑coding RNA DLEU2 suppresses idiopathic pulmonary fibrosis by regulating the microRNA‑369‑3p/TRIM2 axis

  • Authors:
    • Hengzhong Yi
    • Danlin Luo
    • Yangbao Xiao
    • Di Jiang
  • View Affiliations / Copyright

    Affiliations: Sixth Medical Department, Hunan Chest Hospital, Changsha, Hunan 410013, P.R. China, Endoscopy Center, Hunan Chest Hospital, Changsha, Hunan 410013, P.R. China, Department of Ultrasound, The First Affiliated Hospital of University of South China, Hengyang, Hunan 421001, P.R. China
    Copyright: © Yi et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Article Number: 80
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    Published online on: March 11, 2021
       https://doi.org/10.3892/ijmm.2021.4913
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Abstract

Idiopathic pulmonary fibrosis (IPF) is the most common form of idiopathic interstitial pneumonia with an increasing incidence. In the present study, Genome Expression Omnibus (GEO) datasets (GSE10667, GSE24206 and GSE32537) were applied to identify lncRNA DLEU2 in IPF. Through prediction using starBase, TargetScan, miRTarBase and miRDB, tripartite motif containing 2 (TRIM2) and prostaglandin F2 receptor inhibitor (PTGFRN) were found to be upregulated in IPF. DLEU2 expression, the mRNA expression of TRIM2 and PTGFRN, and miR‑369‑3p expression in A549 cells and lung tissues were detected by RT‑qPCR. The protein expression of TRIM2 and PTGFRN in lung tissues and A549 cells was detected by western blot analysis. The proliferation and migration of A549 cells was respectively detected by CCK‑8 assay and wound healing assay. The expression of collagen I, α‑smooth muscle actin (SMA) and E‑cadherin was detected by immunofluorescence assay in A549 cells, and collagen I expression was detected by immunohistochemistry assay in lung tissues. The expression of collagen I, α‑SMA and E‑cadherin was also detected by western blot analysis in A549 cells and lung tissues. Dual‑luciferase reporter assay was used to confirm the association between DLEU2 and miR‑369‑3p, and miR‑369‑3p and TRIM2. As a result, DLEU2 expression was found to be upregulated in IPF and in transforming growth factor (TGF)‑β1‑stimulated A549 cells. The silencing of DLEU2 inhibited the TGF‑β1‑induced proliferation, migration and epithelial‑mesenchymal transition (EMT) of A549 cells and bleomycin (BLM)‑induced pulmonary fibrosis in mice. TRIM2 expression was increased and miR‑369‑3p expression was decreased in the lung tissues of mice with BLM‑induced fibrosis and in TGF‑β1‑stimulated A549 cells. DLEU2 directly targeted miR‑369‑3p. The effect of the silencing of DLEU2 on TGF‑β1‑stimulated A549 cells was suppressed by the silencing of miR‑369‑3p. TRIM2 was the target protein of miR‑369‑3p. On the whole, the present study demonstrates that the silencing of DLEU2 suppressed IPF by upregulating miR‑369‑3p expression and downregulating TRIM2 expression.
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1 

Caminati A, Madotto F, Cesana G, Conti S and Harari S: Epidemiological studies in idiopathic pulmonary fibrosis: Pitfalls in methodologies and data interpretation. Eur Respir Rev. 24:436–444. 2015. View Article : Google Scholar : PubMed/NCBI

2 

Chanda D, Otoupalova E, Smith SR, Volckaert T, De Langhe SP and Thannickal VJ: Developmental pathways in the pathogenesis of lung fibrosis. Mol Aspects Med. 65:56–69. 2019. View Article : Google Scholar :

3 

Meyer KC: Pulmonary fibrosis, part I: Epidemiology, pathogenesis, and diagnosis. Expert Rev Respir Med. 11:343–359. 2017.PubMed/NCBI

4 

Johnsson P, Lipovich L, Grandér D and Morris KV: Evolutionary conservation of long non-coding RNAs; Sequence, structure, function. Biochim Biophys Acta. 1840:1063–1071. 2014. View Article : Google Scholar

5 

Sun C, Huang L, Li Z, Leng K, Xu Y, Jiang X and Cui Y: Long non-coding RNA MIAT in development and disease: A new player in an old game. J Biomed Sci. 25:232018. View Article : Google Scholar : PubMed/NCBI

6 

Wu W, Zhao Y, Gao E, Li Y, Guo X, Zhao T, He W and Zhang H: LncRNA DLEU2 accelerates the tumorigenesis and invasion of non-small cell lung cancer by sponging miR-30a-5p. J Cell Mol Med. 24:441–450. 2020. View Article : Google Scholar

7 

Liu W, Liu PC, Ma K, Wang YY, Chi QB and Yan M: LncRNA DLEU2 promotes tumour growth by sponging miR-337-3p in human osteosarcoma. Cell Biochem Funct. 38:886–894. 2020. View Article : Google Scholar : PubMed/NCBI

8 

Wang B, Hang J, Li W and Yuan W: Knockdown of LncRNA DLEU2 inhibits cervical cancer progression via targeting miR-128-3p. Onco Targets Ther. 13:10173–10184. 2020. View Article : Google Scholar : PubMed/NCBI

9 

Lu M, Chen W, Zhuang W and Zhan X: Label-free quantitative identification of abnormally ubiquitinated proteins as useful biomarkers for human lung squamous cell carcinomas. EPMA J. 11:73–94. 2020. View Article : Google Scholar : PubMed/NCBI

10 

Cao H, Fang Y, Liang Q, Wang J, Luo B, Zeng G, Zhang T, Jing X and Wang X: TRIM2 is a novel promoter of human colorectal cancer. Scand J Gastroenterol. 54:210–218. 2019. View Article : Google Scholar : PubMed/NCBI

11 

Qin Y, Ye J, Zhao F, Hu S and Wang S: TRIM2 regulates the development and metastasis of tumorous cells of osteosarcoma. Int J Oncol. 53:1643–1656. 2018.PubMed/NCBI

12 

Dong R, Liu M, Huang XX, Liu Z, Jiang DY, Xiao HJ, Geng J, Ren YH and Dai HP: Water-Soluble C60 protects against bleomycin-induced pulmonary fibrosis in mice. Int J Nanomedicine. 15:2269–2276. 2020. View Article : Google Scholar :

13 

Livak KJ and Schmittgen TD: Analysis of relative gene expression data using real-time quantitative PCR and the 2(-Delta Delta C(T)) method. Methods. 25:402–408. 2001. View Article : Google Scholar

14 

Tang F, Li R, Xue J, Lan J, Xu H, Liu Y, Zhou L and Lu Y: Azithromycin attenuates acute radiation-induced lung injury in mice. Oncol Lett. 14:5211–5220. 2017.PubMed/NCBI

15 

Abdelfattah MS, Elmallah MIY, Ebrahim HY, Almeer RS, Eltanany RMA and Abdel Moneim AE: Prodigiosins from a marine sponge-associated actinomycete attenuate HCl/ethanol-induced gastric lesion via antioxidant and anti-inflammatory mechanisms. PLoS One. 14:e02167372019. View Article : Google Scholar : PubMed/NCBI

16 

Cookson WO and Moffatt MF: Bedside to gene and back in idiopathic pulmonary fibrosis. N Engl J Med. 368:2228–2230. 2013. View Article : Google Scholar : PubMed/NCBI

17 

Fernandez IE and Eickelberg O: New cellular and molecular mechanisms of lung injury and fibrosis in idiopathic pulmonary fibrosis. Lancet. 380:680–688. 2012. View Article : Google Scholar : PubMed/NCBI

18 

Fell CD, Martinez FJ, Liu LX, Murray S, Han MK, Kazerooni EA, Gross BH, Myers J, Travis WD, Colby TV, et al: Clinical predictors of a diagnosis of idiopathic pulmonary fibrosis. Am J Respir Crit Care Med. 181:832–837. 2010. View Article : Google Scholar : PubMed/NCBI

19 

Baumgart M, Groth M, Priebe S, Savino A, Testa G, Dix A, Ripa R, Spallotta F, Gaetano C, Ori M, et al: RNA-seq of the aging brain in the short-lived fish N. furzeri-conserved pathways and novel genes associated with neurogenesis. Aging Cell. 13:965–974. 2014. View Article : Google Scholar : PubMed/NCBI

20 

Benayoun BA, Pollina EA and Brunet A: Epigenetic regulation of ageing: Linking environmental inputs to genomic stability. Nat Rev Mol Cell Biol. 16:593–610. 2015. View Article : Google Scholar : PubMed/NCBI

21 

Grammatikakis I, Panda AC, Abdelmohsen K and Gorospe M: Long noncoding RNAs(lncRNAs) and the molecular hallmarks of aging. Aging (Albany NY). 6:992–1009. 2014. View Article : Google Scholar

22 

Huang C, Yang Y and Liu L: Interaction of long noncoding RNAs and microRNAs in the pathogenesis of idiopathic pulmonary fibrosis. Physiol Genomics. 47:463–469. 2015. View Article : Google Scholar : PubMed/NCBI

23 

Xie Z, Li X, Chen H, Zeng A, Shi Y and Tang Y: The lncRNA-DLEU2/miR-186-5p/PDK3 axis promotes the progress of glioma cells. Am J Transl Res. 11:4922–4934. 2019.PubMed/NCBI

24 

Guo Y, Bai M, Lin L, Huang J, An Y, Liang L, Liu Y and Huang W: LncRNA DLEU2 aggravates the progression of hepatocellular carcinoma through binding. Biomed Pharmacother. 118:1092722019. View Article : Google Scholar

25 

Lu T, Wang R, Cai H and Cui Y: Long non-coding RNA DLEU2 promotes the progression of esophageal cancer through miR-30e-5p/E2F7 axis. Biomed Pharmacother. 123:1096502020. View Article : Google Scholar

26 

Li C, Wang Z, Zhang J, Zhao X, Xu P, Liu X, Li M, Lv C and Song X: Crosstalk of mRNA, miRNA, lncRNA, and circRNA and their regulatory pattern in pulmonary fibrosis. Mol Ther Nucleic Acids. 18:204–218. 2019. View Article : Google Scholar : PubMed/NCBI

27 

Liu P, Ma C, Wu Q, Zhang W, Wang C, Yuan L and Xi X: MiR-369-3p participates in endometrioid adenocarcinoma via the regulation of autophagy. Cancer Cell Int. 19:1782019. View Article : Google Scholar : PubMed/NCBI

28 

Pan W, Yu H, Zheng B, Gao Y, Li P, Huang Q, Xie C and Ge X: Upregulation of MiR-369-3p suppresses cell migration and proliferation by targeting SOX4 in Hirschsprung's disease. J Pediatr Surg. 52:1363–1370. 2017. View Article : Google Scholar : PubMed/NCBI

29 

Xu Q and Liu K: MiR-369-3p inhibits tumorigenesis of hepatocellular carcinoma by binding to PAX6. J Biol Regul Homeost Agents. 34:917–926. 2020.PubMed/NCBI

30 

Scalavino V, Liso M, Cavalcanti E, Gigante I, Lippolis A, Mastronardi M, Chieppa M and Serino G: miR-369-3p modulates inducible nitric oxide synthase and is involved in regulation of chronic inflammatory response. Sci Rep. 10:159422020. View Article : Google Scholar : PubMed/NCBI

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Copy and paste a formatted citation
Spandidos Publications style
Yi H, Luo D, Xiao Y and Jiang D: Knockdown of long non‑coding RNA DLEU2 suppresses idiopathic pulmonary fibrosis by regulating the microRNA‑369‑3p/TRIM2 axis. Int J Mol Med 47: 80, 2021.
APA
Yi, H., Luo, D., Xiao, Y., & Jiang, D. (2021). Knockdown of long non‑coding RNA DLEU2 suppresses idiopathic pulmonary fibrosis by regulating the microRNA‑369‑3p/TRIM2 axis. International Journal of Molecular Medicine, 47, 80. https://doi.org/10.3892/ijmm.2021.4913
MLA
Yi, H., Luo, D., Xiao, Y., Jiang, D."Knockdown of long non‑coding RNA DLEU2 suppresses idiopathic pulmonary fibrosis by regulating the microRNA‑369‑3p/TRIM2 axis". International Journal of Molecular Medicine 47.5 (2021): 80.
Chicago
Yi, H., Luo, D., Xiao, Y., Jiang, D."Knockdown of long non‑coding RNA DLEU2 suppresses idiopathic pulmonary fibrosis by regulating the microRNA‑369‑3p/TRIM2 axis". International Journal of Molecular Medicine 47, no. 5 (2021): 80. https://doi.org/10.3892/ijmm.2021.4913
Copy and paste a formatted citation
x
Spandidos Publications style
Yi H, Luo D, Xiao Y and Jiang D: Knockdown of long non‑coding RNA DLEU2 suppresses idiopathic pulmonary fibrosis by regulating the microRNA‑369‑3p/TRIM2 axis. Int J Mol Med 47: 80, 2021.
APA
Yi, H., Luo, D., Xiao, Y., & Jiang, D. (2021). Knockdown of long non‑coding RNA DLEU2 suppresses idiopathic pulmonary fibrosis by regulating the microRNA‑369‑3p/TRIM2 axis. International Journal of Molecular Medicine, 47, 80. https://doi.org/10.3892/ijmm.2021.4913
MLA
Yi, H., Luo, D., Xiao, Y., Jiang, D."Knockdown of long non‑coding RNA DLEU2 suppresses idiopathic pulmonary fibrosis by regulating the microRNA‑369‑3p/TRIM2 axis". International Journal of Molecular Medicine 47.5 (2021): 80.
Chicago
Yi, H., Luo, D., Xiao, Y., Jiang, D."Knockdown of long non‑coding RNA DLEU2 suppresses idiopathic pulmonary fibrosis by regulating the microRNA‑369‑3p/TRIM2 axis". International Journal of Molecular Medicine 47, no. 5 (2021): 80. https://doi.org/10.3892/ijmm.2021.4913
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