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Ceramide induces the apoptosis of non‑small cell lung cancer cells through the Txnip/Trx1 complex

  • Authors:
    • Yining Shi
    • Yongmei Jin
    • Fangfang Liu
    • Jianjun Jiang
    • Jiyu Cao
    • Youjin Lu
    • Jin Yang
  • View Affiliations / Copyright

    Affiliations: Department of Respiratory Medicine, The Second Hospital of Anhui Medical University, Hefei, Anhui 230022, P.R. China, Department of Respiratory Medicine, The Second People's Hospital of Hefei, Hefei, Anhui 230022, P.R. China, Department of Respiratory Medicine, The First Hospital of Anhui Medical University, Hefei, Anhui 230022, P.R. China, The Teaching Center for Preventive Medicine, School of Public Health, Anhui Medical University, Hefei, Anhui 230022, P.R. China
    Copyright: © Shi et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Article Number: 85
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    Published online on: March 19, 2021
       https://doi.org/10.3892/ijmm.2021.4918
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Abstract

Ceramide is a biologically active sphingomyelin that inhibits cell growth and proliferation. In previous studies, it was demonstrated that the use of lipopolysaccharides induces acid sphingomyelinases to produce ceramide, promoting lung cancer cell apoptosis; however, the specific mechanisms of this action remain unclear. Thioredoxin‑interacting protein (Txnip) plays an important role in the signal transmission of redox reactions inside and outside the cell. Thus, it was hypothesized that ceramide induces apoptosis in lung adenocarcinoma cells (A549 and PC9) by modulating the Txnip/Trx1 complex. In the present study, the Cell Counting kit‑8 method was used to detect cell activity and the drug concentration. Hoechst 33258 staining and flow cytometry were used to detect cell apoptosis, and the positional association between Txnip and Trx1 upregulated by ceramide was observed by immunofluorescence confocal microscopy. Reverse transcription‑quantitative polymerase chain reaction and western blot analysis were used to detect the changes in related gene, mRNA and protein expression levels. The results revealed that ceramide treatment resulted in the upregulation of Txnip and in the reduction of Trx1 activities. However, the Txnip inhibitor, verapamil, reversed these changes. The analysis of mRNA expression further verified the changes observed in the protein expression of Txnip, Trx1 and apoptosis‑related proteins. On the whole, the present study demonstrates that ceramide induces the apoptosis of lung cancer cells by regulating the Txnip/Trx1 complex.
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View References

1 

Remark R, Becker C, Gomez JE, Damotte D, Dieu-Nosjean MC, Sautès-Fridman C, Fridman WH, Powell CA, Altorki NK, Merad M and Gnjatic S: The non-small cell lung cancer immune contexture. A major determinant of tumor characteristics and patient outcome. Am J Respir Crit Care Med. 191:377–390. 2015. View Article : Google Scholar

2 

Skřičková J, Kadlec B, Venclíček O and Merta Z: Lung cancer. Cas Lek Cesk. 157:226–236. 2018.

3 

Misra P and Singh S: Role of cytokines in combinatorial immunotherapeutics of non-small cell lung cancer through systems perspective. Cancer Med. 8:1976–1995. 2019. View Article : Google Scholar : PubMed/NCBI

4 

Naylor EC, Desani JK and Chung PK: Targeted therapy and immunotherapy for lung cancer. Surg Oncol Clin N Am. 25:601–609. 2016. View Article : Google Scholar : PubMed/NCBI

5 

Owen DH, Williams TM, Bertino EM, Mo X, Webb A, Schweitzer C, Liu T, Roychowdhury S, Timmers CD and Otterson GA: Homologous recombination and DNA repair mutations in patients treated with carboplatin and nab-paclitaxel for metastatic non-small cell lung cancer. Lung Cancer. 134:167–173. 2019. View Article : Google Scholar : PubMed/NCBI

6 

Kuş G, Özkurt M, Öztopcu Vatan P, Erkasap N, Uyar R and Kabadere S: Comparison of a ceramidase inhibitor (ceranib-2) with C2 ceramide and cisplatin on cytotoxicity and apoptosis of glioma cells. Turk J Biol. 42:259–265. 2018.

7 

Ordoñez R, Fernández A, Prieto-Domínguez N, Martínez L, García-Ruiz C, Fernández-Checa JC, Mauriz JL and González-Gallego J: Ceramide metabolism regulates autophagy and apoptotic cell death induced by melatonin in liver cancer cells. J Pineal Res. 59:178–189. 2015. View Article : Google Scholar : PubMed/NCBI

8 

Kolesnick R and Fuks Z: Radiation and ceramide-induced apoptosis. Oncogene. 22:5897–5906. 2003. View Article : Google Scholar : PubMed/NCBI

9 

Brodowicz J, Przegaliński E, Müller CP and Filip M: Ceramide and its related neurochemical networks as targets for some brain disorder therapies. Neurotox Res. 33:474–484. 2018. View Article : Google Scholar :

10 

Chang YC, Fong Y, Tsai EM, Chang YG, Chou HL, Wu CY, Teng YN, Liu TC, Yuan SS and Chiu CC: Exogenous C8-ceramide induces apoptosis by overproduction of ROS and the switch of superoxide dismutases SOD1 to SOD2 in human lung cancer cells. Int J Mol Sci. 19:30102018. View Article : Google Scholar

11 

Ma JQ, Liu CM and Yang W: Protective effect of rutin against carbon tetrachloride-induced oxidative stress, inflammation and apoptosis in mouse kidney associated with the ceramide, MAPKs, p53 and calpain activities. Chem Biol Interact. 286:26–33. 2018. View Article : Google Scholar : PubMed/NCBI

12 

Maeng HJ, Song JH, Kim GT, Song YJ, Lee K, Kim JY and Park TS: Celecoxib-mediated activation of endoplasmic reticulum stress induces de novo ceramide biosynthesis and apoptosis in hepatoma HepG2 cells mobilization. BMB Rep. 50:144–149. 2017. View Article : Google Scholar : PubMed/NCBI

13 

Wang SW, Hojabrpour P, Zhang P, Kolesnick RN, Steinbrecher UP, Gómez-Muñoz A and Duronio V: Regulation of ceramide generation during macrophage apoptosis by ASMase and de novo synthesis. Biochim Biophys Acta. 1851:1482–1489. 2015. View Article : Google Scholar : PubMed/NCBI

14 

Dai L, Smith CD, Foroozesh M, Miele L and Qin Z: The sphingosine kinase 2 inhibitor ABC294640 displays anti-non-small cell lung cancer activities in vitro and in vivo. Int J Cancer. 142:2153–2162. 2018. View Article : Google Scholar :

15 

Chang WT, Wu CY, Lin YC, Wu MT, Su KL, Yuan SS, Wang HD, Fong Y, Lin YH and Chiu CC: C(2)-ceramide-induced Rb-dominant senescence-like phenotype leads to human breast cancer MCF-7 escape from p53-dependent cell death. Int J Mol Sci. 20:42922019. View Article : Google Scholar

16 

Rahman A, Pallichankandy S, Thayyullathil F and Galadari S: Critical role of H(2)O(2) in mediating sanguinarine-induced apoptosis in prostate cancer cells via facilitating ceramide generation, ERK1/2 phosphorylation, and Par-4 cleavage. Free Radic Biol Med. 134:527–544. 2019. View Article : Google Scholar : PubMed/NCBI

17 

Yildiz-Ozer M, Oztopcu-Vatan P and Kus G: The investigation of ceranib-2 on apoptosis and drug interaction with carboplatin in human non small cell lung cancer cells in vitro. Cytotechnology. 70:387–396. 2018. View Article : Google Scholar :

18 

Suzuki M, Cao K, Kato S, Komizu Y, Mizutani N, Tanaka K, Arima C, Tai MC, Yanagisawa K, Togawa N, et al: Targeting ceramide synthase 6-dependent metastasis-prone phenotype in lung cancer cells. J Clin Invest. 126:254–265. 2016. View Article : Google Scholar

19 

Xu L, Lin X, Guan M, Zeng Y and Liu Y: Verapamil attenuated prediabetic neuropathy in high-fat diet-fed mice through inhibiting TXNIP-mediated apoptosis and inflammation. Oxid Med Cell Longev. 2019:18960412019. View Article : Google Scholar : PubMed/NCBI

20 

Kizhakkayil J, Thayyullathil F, Chathoth S, Hago A, Patel M and Galadari S: Glutathione regulates caspase-dependent ceramide production and curcumin-induced apoptosis in human leukemic cells. Free Radic Biol Med. 52:1854–1864. 2012. View Article : Google Scholar : PubMed/NCBI

21 

Xu W, Wang L, Li J, Cai Y and Xue Y: TXNIP mediated the oxidative stress response in glomerular mesangial cells partially through AMPK pathway. Biomed Pharmacother. 107:785–792. 2018. View Article : Google Scholar : PubMed/NCBI

22 

Lv H, Zhu C, Wei W, Lv X, Yu Q, Deng X and Ci X: Enhanced Keap1-Nrf2/Trx-1 axis by daphnetin protects against oxidative stress-driven hepatotoxicity via inhibiting ASK1/JNK and Txnip/NLRP3 inflammasome activation. Phytomedicine. 71:1532412020. View Article : Google Scholar : PubMed/NCBI

23 

Yu Y, Xing K, Badamas R, Kuszynski CA, Wu H and Lou MF: Overexpression of thioredoxin-binding protein 2 increases oxidation sensitivity and apoptosis in human lens epithelial cells. Free Radic Biol Med. 57:92–104. 2013. View Article : Google Scholar : PubMed/NCBI

24 

Muri J, Heer S, Matsushita M, Pohlmeier L, Tortola L, Fuhrer T, Conrad M, Zamboni N, Kisielow J and Kopf M: The thioredoxin-1 system is essential for fueling DNA synthesis during T-cell metabolic reprogramming and proliferation. Nat Commun. 9:18512018. View Article : Google Scholar : PubMed/NCBI

25 

Ungerstedt J, Du Y, Zhang H, Nair D and Holmgren A: In vivo redox state of human thioredoxin and redox shift by the histone deacetylase inhibitor suberoylanilide hydroxamic acid (SAHA). Free Radic Biol Med. 53:2002–2007. 2012. View Article : Google Scholar : PubMed/NCBI

26 

Cohen-Kutner M, Khomsky L, Trus M, Ben-Yehuda H, Lenhard JM, Liang Y, Martin T and Atlas D: Thioredoxin-mimetic peptide CB3 lowers MAPKinase activity in the Zucker rat brain. Redox Biol. 2:447–456. 2014. View Article : Google Scholar : PubMed/NCBI

27 

Hou Y, Wang Y, He Q, Li L, Xie H, Zhao Y and Zhao J: Nrf2 inhibits NLRP3 inflammasome activation through regulating Trx1/TXNIP complex in cerebral ischemia reperfusion injury. Behav Brain Res. 336:32–39. 2018. View Article : Google Scholar

28 

Livak KJ and Schmittgen TD: Analysis of relative gene expression data using real-time quantitative PCR and the 2(-Delta Delta C(T)) method. Methods. 25:402–408. 2001. View Article : Google Scholar

29 

Morad SA, Davis TS, MacDougall MR, Tan SF, Feith DJ, Desai DH, Amin SG, Kester M, Loughran TP Jr and Cabot MC: Role of P-glycoprotein inhibitors in ceramide-based therapeutics for treatment of cancer. Biochem Pharmacol. 130:21–33. 2017. View Article : Google Scholar : PubMed/NCBI

30 

Liu H, Yang J, Wang Y, Wei Y, Cao J and Lu Y: C8-ceramide induces apoptosis of alveolar type II epithelial cells. Chinese J Tuberculosis Respir Zhonghua Jie He He Hu Xi Za Zhi. 38:445–450. 2015.In Chinese.

31 

Yang J, Wang Y, Liu H, Bi J and Lu Y: C2-ceramide influences alveolar epithelial barrier function by downregulating Zo-1, occludin and claudin-4 expression. Toxicol Mech Methods. 27:293–297. 2017. View Article : Google Scholar : PubMed/NCBI

32 

Siegel RL, Miller KD and Jemal A: Cancer statistics, 2019. CA Cancer J Clin. 69:7–34. 2019. View Article : Google Scholar : PubMed/NCBI

33 

Mattiuzzi C and Lippi G: Current cancer epidemiology. J Epidemiol Glob Health. 9:217–222. 2019. View Article : Google Scholar : PubMed/NCBI

34 

Du A, Jiang Y and Fan C: NDRG1 downregulates ATF3 and inhibits cisplatin-induced cytotoxicity in lung cancer A549 cells. Int J Med Sci. 15:1502–1507. 2018. View Article : Google Scholar : PubMed/NCBI

35 

Ogretmen B: Sphingolipid metabolism in cancer signalling and therapy. Nat Rev Cancer. 18:33–50. 2018. View Article : Google Scholar :

36 

Che J, Huang Y, Xu C and Zhang P: Increased ceramide production sensitizes breast cancer cell response to chemotherapy. Cancer Chemother Pharmacol. 79:933–941. 2017. View Article : Google Scholar : PubMed/NCBI

37 

World C, Spindel ON and Berk BC: Thioredoxin-interacting protein mediates TRX1 translocation to the plasma membrane in response to tumor necrosis factor-α: A key mechanism for vascular endothelial growth factor receptor-2 transactivation by reactive oxygen species. Arterioscler Thromb Vasc Biol. 31:1890–1897. 2011. View Article : Google Scholar : PubMed/NCBI

38 

Bharti V, Tan H, Zhou H and Wang JF: Txnip mediates glucocorticoid-activated NLRP3 inflammatory signaling in mouse microglia. Neurochem Int. 131:1045642019. View Article : Google Scholar : PubMed/NCBI

39 

Hou R, Shen M, Wang R, Liu H, Gao C, Xu J, Tao L, Yin Z and Yin T: Thioredoxin1 inactivation mediates the impairment of ischemia-induced angiogenesis and further injury in diabetic myocardium. J Vasc Res. 57:76–85. 2020. View Article : Google Scholar : PubMed/NCBI

40 

Yoshihara E: TXNIP/TBP-2: A master regulator for glucose homeostasis. Antioxidants (Basel). 9:7652020. View Article : Google Scholar

41 

Nishiyama A, Matsui M, Iwata S, Hirota K, Masutani H, Nakamura H, Takagi Y, Sono H, Gon Y and Yodoi J: Identification of thioredoxin-binding protein-2/vitamin D(3) up-regulated protein 1 as a negative regulator of thioredoxin function and expression. J Biol Chem. 274:21645–21650. 1999. View Article : Google Scholar : PubMed/NCBI

42 

Wang Y, De Keulenaer GW and Lee RT: Vitamin D(3)-up-regulated protein-1 is a stress-responsive gene that regulates cardiomyocyte viability through interaction with thioredoxin. J Biol Chem. 277:26496–26500. 2002. View Article : Google Scholar : PubMed/NCBI

43 

Li D, Ren W, Jiang Z and Zhu L: Regulation of the NLRP3 inflammasome and macrophage pyroptosis by the p38 MAPK signaling pathway in a mouse model of acute lung injury. Mol Med Rep. 18:4399–4409. 2018.PubMed/NCBI

44 

Song W, Wei L, Du Y, Wang Y and Jiang S: Protective effect of ginsenoside metabolite compound K against diabetic nephropathy by inhibiting NLRP3 inflammasome activation and NF-κB/p38 signaling pathway in high-fat diet/streptozotocin-induced diabetic mice. Int Immunopharmacol. 63:227–238. 2018. View Article : Google Scholar : PubMed/NCBI

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Copy and paste a formatted citation
Spandidos Publications style
Shi Y, Jin Y, Liu F, Jiang J, Cao J, Lu Y and Yang J: Ceramide induces the apoptosis of non‑small cell lung cancer cells through the Txnip/Trx1 complex. Int J Mol Med 47: 85, 2021.
APA
Shi, Y., Jin, Y., Liu, F., Jiang, J., Cao, J., Lu, Y., & Yang, J. (2021). Ceramide induces the apoptosis of non‑small cell lung cancer cells through the Txnip/Trx1 complex. International Journal of Molecular Medicine, 47, 85. https://doi.org/10.3892/ijmm.2021.4918
MLA
Shi, Y., Jin, Y., Liu, F., Jiang, J., Cao, J., Lu, Y., Yang, J."Ceramide induces the apoptosis of non‑small cell lung cancer cells through the Txnip/Trx1 complex". International Journal of Molecular Medicine 47.5 (2021): 85.
Chicago
Shi, Y., Jin, Y., Liu, F., Jiang, J., Cao, J., Lu, Y., Yang, J."Ceramide induces the apoptosis of non‑small cell lung cancer cells through the Txnip/Trx1 complex". International Journal of Molecular Medicine 47, no. 5 (2021): 85. https://doi.org/10.3892/ijmm.2021.4918
Copy and paste a formatted citation
x
Spandidos Publications style
Shi Y, Jin Y, Liu F, Jiang J, Cao J, Lu Y and Yang J: Ceramide induces the apoptosis of non‑small cell lung cancer cells through the Txnip/Trx1 complex. Int J Mol Med 47: 85, 2021.
APA
Shi, Y., Jin, Y., Liu, F., Jiang, J., Cao, J., Lu, Y., & Yang, J. (2021). Ceramide induces the apoptosis of non‑small cell lung cancer cells through the Txnip/Trx1 complex. International Journal of Molecular Medicine, 47, 85. https://doi.org/10.3892/ijmm.2021.4918
MLA
Shi, Y., Jin, Y., Liu, F., Jiang, J., Cao, J., Lu, Y., Yang, J."Ceramide induces the apoptosis of non‑small cell lung cancer cells through the Txnip/Trx1 complex". International Journal of Molecular Medicine 47.5 (2021): 85.
Chicago
Shi, Y., Jin, Y., Liu, F., Jiang, J., Cao, J., Lu, Y., Yang, J."Ceramide induces the apoptosis of non‑small cell lung cancer cells through the Txnip/Trx1 complex". International Journal of Molecular Medicine 47, no. 5 (2021): 85. https://doi.org/10.3892/ijmm.2021.4918
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