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Knockdown of lncRNA MIR4435‑2HG and ST8SIA1 expression inhibits the proliferation, invasion and migration of prostate cancer cells in vitro and in vivo by blocking the activation of the FAK/AKT/β‑catenin signaling pathway

  • Authors:
    • Pengyi Xing
    • Ye Wang
    • Li Zhang
    • Chao Ma
    • Jianping Lu
  • View Affiliations / Copyright

    Affiliations: Department of Radiology, The First Affiliated Hospital of Naval Medical University, Shanghai 200433, P.R. China, Department of Urology, Chinese People's Liberation Army (PLA) General Hospital/PLA Medical School, Beijing 100853, P.R. China, Department of Endocrinology, PLA Joint Logistics Support Force No. 989 Hospital, Luoyang, Henan 471600, P.R. China
    Copyright: © Xing et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Article Number: 93
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    Published online on: April 1, 2021
       https://doi.org/10.3892/ijmm.2021.4926
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Abstract

Prostate cancer is a main health risk for males with a high incidence and mortality. The present study aimed to examine the effects of long non‑coding RNA (lncRNA) MIR4435‑2HG binding with ST8SIA1 on the proliferation, invasion and migration of prostate cancer cells via the activation of the FAK/AKT/β‑catenin signaling pathway. The expression of MIR4435‑2HG and ST8SIA1 in prostate cancer cell lines, and the transfection efficacy were analyzed by RT‑qPCR. The proliferation, clone formation ability, and the invasion and migration of transfected cells were detected by CCK‑8 assay, clone formation assay, Transwell assay and wound healing assay, respectively. Plasmids were injected subcutaneously into mice to construct a xenograft tumor model. The expression levels of proteins related to proliferation, apoptosis, invasion and migration, and the FAK/AKT/β‑catenin pathway were detected by western blot analysis. The results revealed that MIR4435‑2HG expression was increased in the prostate cancer cell lines and MIR4435‑2HG expression was the highest in the PC‑3 cells. Interference with MIR4435‑2HG inhibited the proliferation, clone formation ability, and the invasion and migration of PC‑3 cells, as well as tumor growth by suppressing the activation of the FAK/AKT/β‑catenin signaling pathway. MIR4435‑2HG was demonstrated to target ST8SIA1. ST8SIA1 expression was also increased in the prostate cancer cell lines and MIR4435‑2HG expression was the highest in the PC‑3 cells. Interference with ST8SIA1 inhibited the promoting effects of MIR4435‑2HG on the proliferation, invasion and migration of PC‑3 cells, as well as tumor growth by suppressing the activation of the FAK/AKT/β‑catenin signaling pathway. On the whole, the present study demonstrates that interference with MIR4435‑2HG, combined with ST8SIA1, inhibits the proliferation, invasion and migration of prostate cancer cells in vitro and in vivo by blocking the activation of the FAK/AKT/β‑catenin signaling pathway.
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Copy and paste a formatted citation
Spandidos Publications style
Xing P, Wang Y, Zhang L, Ma C and Lu J: Knockdown of lncRNA MIR4435‑2HG and ST8SIA1 expression inhibits the proliferation, invasion and migration of prostate cancer cells <em>in vitro</em> and <em>in vivo</em> by blocking the activation of the FAK/AKT/β‑catenin signaling pathway. Int J Mol Med 47: 93, 2021.
APA
Xing, P., Wang, Y., Zhang, L., Ma, C., & Lu, J. (2021). Knockdown of lncRNA MIR4435‑2HG and ST8SIA1 expression inhibits the proliferation, invasion and migration of prostate cancer cells <em>in vitro</em> and <em>in vivo</em> by blocking the activation of the FAK/AKT/β‑catenin signaling pathway. International Journal of Molecular Medicine, 47, 93. https://doi.org/10.3892/ijmm.2021.4926
MLA
Xing, P., Wang, Y., Zhang, L., Ma, C., Lu, J."Knockdown of lncRNA MIR4435‑2HG and ST8SIA1 expression inhibits the proliferation, invasion and migration of prostate cancer cells <em>in vitro</em> and <em>in vivo</em> by blocking the activation of the FAK/AKT/β‑catenin signaling pathway". International Journal of Molecular Medicine 47.6 (2021): 93.
Chicago
Xing, P., Wang, Y., Zhang, L., Ma, C., Lu, J."Knockdown of lncRNA MIR4435‑2HG and ST8SIA1 expression inhibits the proliferation, invasion and migration of prostate cancer cells <em>in vitro</em> and <em>in vivo</em> by blocking the activation of the FAK/AKT/β‑catenin signaling pathway". International Journal of Molecular Medicine 47, no. 6 (2021): 93. https://doi.org/10.3892/ijmm.2021.4926
Copy and paste a formatted citation
x
Spandidos Publications style
Xing P, Wang Y, Zhang L, Ma C and Lu J: Knockdown of lncRNA MIR4435‑2HG and ST8SIA1 expression inhibits the proliferation, invasion and migration of prostate cancer cells <em>in vitro</em> and <em>in vivo</em> by blocking the activation of the FAK/AKT/β‑catenin signaling pathway. Int J Mol Med 47: 93, 2021.
APA
Xing, P., Wang, Y., Zhang, L., Ma, C., & Lu, J. (2021). Knockdown of lncRNA MIR4435‑2HG and ST8SIA1 expression inhibits the proliferation, invasion and migration of prostate cancer cells <em>in vitro</em> and <em>in vivo</em> by blocking the activation of the FAK/AKT/β‑catenin signaling pathway. International Journal of Molecular Medicine, 47, 93. https://doi.org/10.3892/ijmm.2021.4926
MLA
Xing, P., Wang, Y., Zhang, L., Ma, C., Lu, J."Knockdown of lncRNA MIR4435‑2HG and ST8SIA1 expression inhibits the proliferation, invasion and migration of prostate cancer cells <em>in vitro</em> and <em>in vivo</em> by blocking the activation of the FAK/AKT/β‑catenin signaling pathway". International Journal of Molecular Medicine 47.6 (2021): 93.
Chicago
Xing, P., Wang, Y., Zhang, L., Ma, C., Lu, J."Knockdown of lncRNA MIR4435‑2HG and ST8SIA1 expression inhibits the proliferation, invasion and migration of prostate cancer cells <em>in vitro</em> and <em>in vivo</em> by blocking the activation of the FAK/AKT/β‑catenin signaling pathway". International Journal of Molecular Medicine 47, no. 6 (2021): 93. https://doi.org/10.3892/ijmm.2021.4926
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