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Article Open Access

DNAJC12 promotes lung cancer growth by regulating the activation of β‑catenin

  • Authors:
    • Yun Li
    • Meng Li
    • Fengqi Jin
    • Jianbo Liu
    • Minghui Chen
    • Jingjing Yin
  • View Affiliations / Copyright

    Affiliations: School of Medicine, Cheeloo College of Medicine, Shandong University, Jinan, Shandong 250012, P.R. China, Department of Thoracic Surgery, The First People's Hospital of Taian Affiliated to Shandong First Medical University, Taian, Shandong 271000, P.R. China, Department of Thoracic Surgery, Jinan Zhangqiu District Hospital of Traditional Chinese Medicine, Jinan, Shandong 250200, P.R. China, Department of Thoracic Surgery, The Fourth People's Hospital, Heze, Shangdong 274100, P.R. China, Department of Anesthesia Surgery, Shandong Provincial Hospital Affiliated to Shandong First Medical University, Jinan, Shandong 271000, P.R. China, Department of Thoracic Surgery, Shandong Provincial Hospital, Cheeloo College of Medicine, Shandong University, Jinan, Shandong 250021, P.R. China
    Copyright: © Li et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Article Number: 105
    |
    Published online on: April 16, 2021
       https://doi.org/10.3892/ijmm.2021.4938
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Abstract

Lung cancer has become the leading cause of cancer‑associated mortality worldwide. However, the underlying mechanisms of lung cancer remain poorly understood. DnaJ heat shock protein family (HSP40) member C12 (DNAJC12) is a type III member belonging to the HSP40/DNAJ family. The role of DNAJC12 in numerous types of cancer has been previously reported; however, the effect of DNAJC12 in lung cancer remains unknown. The results of the present study indicated that DNAJC12 may be involved in lung cancer proliferation and migration by regulating the β‑catenin signaling pathway. Data generated in the present study and from The Cancer Genome Atlas revealed that the DNAJC12 expression levels were significantly upregulated in lung cancer tissues compared with non‑cancer lung tissues. The expression of DNAJC12 was subsequently knocked down in A549 and NCI‑H1975 lung cancer cells using lentiviral transfections and further experiments demonstrated that the knockdown of DNAJC12 inhibited the proliferation, colony formation, migration and invasion of lung cancer cells. The results of flow cytometric assays also revealed that the knockdown of DNAJC12 induced the apoptosis of lung cancer cells. In addition, the effects of DNAJC12 knockdown on the in vivo growth of lung cancer cells were observed. Signaling pathway analysis revealed that the knockdown of DNAJC12 expression suppressed the phosphorylation of p65 NF‑κB, downregulated the expression levels and inhibited the subsequent activation of β‑catenin, and downregulated the expression levels of vimentin. Rescue experiments demonstrated that the overexpression of β‑catenin, but not that of NF‑κB or vimentin, reversed the effects of DNAJC12 knockdown on the proliferation and invasion of lung cancer cells. On the whole, the findings of the present study suggest that DNAJC12 may play a crucial role in lung cancer tumorigenesis by regulating the expression and activation of β‑catenin. Therefore, DNAJC12 may represent a novel target for the treatment of lung cancer.
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View References

1 

Long K and Suresh K: Pulmonary toxicity of systemic lung cancer therapy. Respirology. 25(Suppl 2): 72–79. 2020. View Article : Google Scholar : PubMed/NCBI

2 

Minna JD, Roth JA and Gazdar AF: Focus on lung cancer. Cancer Cell. 1:49–52. 2002. View Article : Google Scholar : PubMed/NCBI

3 

Tang X, Mo C, Wang Y, Wei D and Xiao H: Anti-tumour strategies aiming to target tumour-associated macrophages. Immunology. 138:93–104. 2013. View Article : Google Scholar :

4 

Roosen DA, Blauwendraat C, Cookson MR and Lewis PA: DNAJC proteins and pathways to parkinsonism. Febs J. 286:3080–3094. 2019. View Article : Google Scholar : PubMed/NCBI

5 

Zylicz M, Yamamoto T, McKittrick N, Sell S and Georgopoulos C: Purification and properties of the dnaJ replication protein of Escherichia coli. J Biol Chem. 260:7591–7598. 1985. View Article : Google Scholar : PubMed/NCBI

6 

Choi J, Djebbar S, Fournier A and Labrie C: The co-chaperone DNAJC12 binds to Hsc70 and is upregulated by endoplasmic reticulum stress. Cell Stress Chaperones. 19:439–446. 2014. View Article : Google Scholar :

7 

Anikster Y, Haack TB, Vilboux T, Pode-Shakked B, Thöny B, Shen N, Guarani V, Meissner T, Mayatepek E, Trefz FK, et al: Biallelic mutations in DNAJC12 cause hyperphenylalaninemia, dystonia, and intellectual disability. Am J Hum Genet. 100:257–266. 2017. View Article : Google Scholar : PubMed/NCBI

8 

Li M, Yang Q, Yi S, Qin Z, Luo J and Fan X: Two novel mutations in DNAJC12 identified by whole-exome sequencing in a patient with mild hyperphenylalaninemia. Mol Genet Genomic Med. 8:e13032020. View Article : Google Scholar : PubMed/NCBI

9 

Gallego D, Leal F, Gámez A, Castro M, Navarrete R, Sanchez- Lijarcio O, Vitoria I, Bueno-Delgado M, Belanger-Quintana A, Morais A, et al: Pathogenic variants of DNAJC12 and evaluation of the encoded cochaperone as a genetic modifier of hyperphenylalaninemia. Human Mutation. 41:1329–1338. 2020. View Article : Google Scholar : PubMed/NCBI

10 

Fan Y, Yang Zh, Li F, Hu XC, Yue YW, Yang J, Liu YT, Liu H, Wang YL, Shi CH and Xu YM: DNAJC12 mutation is rare in Chinese Han population with Parkinson's disease. Neurobiol Aging. 68:159.e1–159.e2. 2018. View Article : Google Scholar

11 

He HL, Lee YE, Chen HP, Hsing CH, Chang IW, Shiue YL, Lee SW, Hsu CT, Lin LC, Wu TF and Li CF: Overexpression of DNAJC12 predicts poor response to neoadjuvant concurrent chemoradiotherapy in patients with rectal cancer. Exp Mol Pathol. 98:338–345. 2015. View Article : Google Scholar : PubMed/NCBI

12 

Uno Y, Kanda M, Miwa T, Umeda S, Tanaka H, Tanaka C, Kobayashi D, Suenaga M, Hattori N, Hayashi M, et al: Increased expression of DNAJC12 is associated with aggressive phenotype of gastric cancer. Ann Surg Oncol. 26:836–844. 2019. View Article : Google Scholar : PubMed/NCBI

13 

De Bessa SA, Salaorni S, Patrão DF, Neto MM, Brentani MM and Nagai MA: JDP1 (DNAJC12/Hsp40) expression in breast cancer and its association with estrogen receptor status. Int J Mol Med. 17:363–367. 2006.PubMed/NCBI

14 

Martin-Orozco E, Sanchez-Fernandez A, Ortiz-Parra I and Ayala-San Nicolas M: WNT Signaling in tumors: The way to evade drugs and immunity. Front Immunol. 10:28542019. View Article : Google Scholar

15 

Peifer M, McCrea PD, Green KJ, Wieschaus E and Gumbiner BM: The vertebrate adhesive junction proteins beta-catenin and plakoglobin and the Drosophila segment polarity gene armadillo form a multigene family with similar properties. J Cell Biol. 118:681–691. 1992. View Article : Google Scholar : PubMed/NCBI

16 

Haseeb M, Pirzada RH, Ain QU and Choi S: Wnt signaling in the regulation of immune cell and cancer therapeutics. Cells. 8:13802019. View Article : Google Scholar

17 

Stewart DJ: Wnt signaling pathway in non-small cell lung cancer. J Natl Cancer Inst. 106:djt3562014. View Article : Google Scholar

18 

Krishnamurthy N and Kurzrock R: Targeting the Wnt/beta-catenin pathway in cancer: Update on effectors and inhibitors. Cancer Treat Rev. 62:50–60. 2018. View Article : Google Scholar

19 

Nakayama S, Sng N, Carretero J, Welner R, Hayashi Y, Yamamoto M, Tan AJ, Yamaguchi N, Yasuda H, Li D, et al: β-catenin contributes to lung tumor development induced by EGFR mutations. Cancer Res. 74:5891–5902. 2014. View Article : Google Scholar : PubMed/NCBI

20 

Tang X, Ma H, Han L, Zheng W, Lu YB, Chen XF, Liang ST, Wei GH, Zhang ZQ, Chen HZ and Liu DP: SIRT1 deacetylates the cardiac transcription factor Nkx2.5 and inhibits its transcriptional activity. Sci Rep. 6:365762016. View Article : Google Scholar : PubMed/NCBI

21 

Livak KJ and Schmittgen TD: Analysis of relative gene expres- sion data using real-time quantitative PCR and the 2(-Delta Delta C(T)) method. Methods. 25:402–408. 2001. View Article : Google Scholar

22 

Cui X, Song L, Bai Y, Wang Y, Wang B and Wang W: Stromal interaction molecule 1 regulates growth, cell cycle, and apoptosis of human tongue squamous carcinoma cells. Biosci Rep. 37:BSR201605192017. View Article : Google Scholar : PubMed/NCBI

23 

Wu R, Zhao B, Ren X, Wu S, Liu M, Wang Z and Liu W: MiR-27a-3p targeting GSK3β promotes triple-negative breast cancer proliferation and migration through Wnt/β-catenin pathway. Cancer Manag Res. 12:6241–6249. 2020. View Article : Google Scholar :

24 

Kim JY, Kim HJ, Jung CW, Lee TS, Kim EH and Park MJ: CXCR4 uses STAT3-mediated slug expression to maintain radioresistance of non-small cell lung cancer cells: Emerges as a potential prognostic biomarker for lung cancer. Cell Death Dis. 12:482021. View Article : Google Scholar : PubMed/NCBI

25 

Sivaraman A, Kim D, Bhattarai D, Kim M, Lee HY, Lim S, Kong J, Goo JI, Shim S, Lee S, et al: Synthesis and structure-activity relationships of arylsulfonamides as AIMP2-DX2 inhibitors for the development of a novel anticancer therapy. J Med Chem. 63:5139–5158. 2020. View Article : Google Scholar : PubMed/NCBI

26 

Liang G, Meng W, Huang X, Zhu W, Yin C, Wang C, Fassan M, Yu Y, Kudo M, Xiao S, et al: miR-196b-5p-mediated downregulation of TSPAN12 and GATA6 promotes tumor progression in non-small cell lung cancer. Proc Natl Acad Sci USA. 117:4347–4357. 2020. View Article : Google Scholar : PubMed/NCBI

27 

Hanahan D and Weinberg RA: Hallmarks of cancer: The next generation. Cell. 144:646–674. 2011. View Article : Google Scholar : PubMed/NCBI

28 

Paolillo M and Schinelli S: Extracellular matrix alterations in metastatic processes. Int J Mol Sci. 20:49472019. View Article : Google Scholar :

29 

Wong KK, Jacks T and Dranoff G: NF-kappaB fans the flames of lung carcinogenesis. Cancer Prev Res (Phila). 3:403–405. 2010. View Article : Google Scholar

30 

Popper HH: Progression and metastasis of lung cancer. Cancer Metastasis Rev. 35:75–91. 2016. View Article : Google Scholar : PubMed/NCBI

31 

Xia Z, Wu S, Wei X, Liao Y, Yi P, Liu Y and Liu J and Liu J: Hypoxic ER stress suppresses β-catenin expression and promotes cooperation between the transcription factors XBP1 and HIF1α for cell survival. J Biol Chem. 294:13811–13821. 2019. View Article : Google Scholar : PubMed/NCBI

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Copy and paste a formatted citation
Spandidos Publications style
Li Y, Li M, Jin F, Liu J, Chen M and Yin J: DNAJC12 promotes lung cancer growth by regulating the activation of β‑catenin. Int J Mol Med 47: 105, 2021.
APA
Li, Y., Li, M., Jin, F., Liu, J., Chen, M., & Yin, J. (2021). DNAJC12 promotes lung cancer growth by regulating the activation of β‑catenin. International Journal of Molecular Medicine, 47, 105. https://doi.org/10.3892/ijmm.2021.4938
MLA
Li, Y., Li, M., Jin, F., Liu, J., Chen, M., Yin, J."DNAJC12 promotes lung cancer growth by regulating the activation of β‑catenin". International Journal of Molecular Medicine 47.6 (2021): 105.
Chicago
Li, Y., Li, M., Jin, F., Liu, J., Chen, M., Yin, J."DNAJC12 promotes lung cancer growth by regulating the activation of β‑catenin". International Journal of Molecular Medicine 47, no. 6 (2021): 105. https://doi.org/10.3892/ijmm.2021.4938
Copy and paste a formatted citation
x
Spandidos Publications style
Li Y, Li M, Jin F, Liu J, Chen M and Yin J: DNAJC12 promotes lung cancer growth by regulating the activation of β‑catenin. Int J Mol Med 47: 105, 2021.
APA
Li, Y., Li, M., Jin, F., Liu, J., Chen, M., & Yin, J. (2021). DNAJC12 promotes lung cancer growth by regulating the activation of β‑catenin. International Journal of Molecular Medicine, 47, 105. https://doi.org/10.3892/ijmm.2021.4938
MLA
Li, Y., Li, M., Jin, F., Liu, J., Chen, M., Yin, J."DNAJC12 promotes lung cancer growth by regulating the activation of β‑catenin". International Journal of Molecular Medicine 47.6 (2021): 105.
Chicago
Li, Y., Li, M., Jin, F., Liu, J., Chen, M., Yin, J."DNAJC12 promotes lung cancer growth by regulating the activation of β‑catenin". International Journal of Molecular Medicine 47, no. 6 (2021): 105. https://doi.org/10.3892/ijmm.2021.4938
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