Open Access

Upregulation of NFKBIZ affects bladder cancer progression via the PTEN/PI3K/Akt signaling pathway

  • Authors:
    • Tao Xu
    • Ting Rao
    • Wei-Ming Yu
    • Jin-Zhuo Ning
    • Xi Yu
    • Shao-Ming Zhu
    • Kang Yang
    • Tao Bai
    • Fan Cheng
  • View Affiliations

  • Published online on: April 19, 2021     https://doi.org/10.3892/ijmm.2021.4942
  • Article Number: 109
  • Copyright: © Xu et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

NF‑κB inhibitor ζ (NFKBIZ), a member of the IκB family that interacts with NF‑κB, has been reported to be an important regulator of inflammation, cell proliferation and survival. However, the role of NFKBIZ in bladder cancer (BC) remains unknown. The present study aimed to investigate the functions of NFKBIZ in BC. First, the expression levels of NFKBIZ and the associations between NFKBIZ expression and the clinical survival of patients were determined using BC tissue samples, BC cell lines and datasets from different databases. Two BC cell lines (T24 and 5637) were selected to overexpress NFKBIZ, and the proliferative, migratory and invasive abilities of cells were determined; additionally, tumor growth following transplantation in in vivo mouse models was analyzed using T24 cells overexpressing NFKBIZ. Subsequently, the association between NFKBIZ and PTEN was determined using data from databases and immunohistochemistry analysis of clinical and nude mice tumor tissues. Finally, the interactions between NFKBIZ, PTEN and the downstream PI3K/AKT/mTOR signaling pathway were evaluated using western blotting. In conclusion, the present results indicated that NFKBIZ expression was low in BC, and NFKBIZ inhibited the proliferation of BC cells through the PTEN/PI3K/Akt signaling pathway, suggesting that NFKBIZ may represent a novel prognostic biomarker in BC and may provide a potential therapeutic tumor‑associated antigen for BC.
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June-2021
Volume 47 Issue 6

Print ISSN: 1107-3756
Online ISSN:1791-244X

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Spandidos Publications style
Xu T, Rao T, Yu W, Ning J, Yu X, Zhu S, Yang K, Bai T and Cheng F: Upregulation of NFKBIZ affects bladder cancer progression via the PTEN/PI3K/Akt signaling pathway. Int J Mol Med 47: 109, 2021
APA
Xu, T., Rao, T., Yu, W., Ning, J., Yu, X., Zhu, S. ... Cheng, F. (2021). Upregulation of NFKBIZ affects bladder cancer progression via the PTEN/PI3K/Akt signaling pathway. International Journal of Molecular Medicine, 47, 109. https://doi.org/10.3892/ijmm.2021.4942
MLA
Xu, T., Rao, T., Yu, W., Ning, J., Yu, X., Zhu, S., Yang, K., Bai, T., Cheng, F."Upregulation of NFKBIZ affects bladder cancer progression via the PTEN/PI3K/Akt signaling pathway". International Journal of Molecular Medicine 47.6 (2021): 109.
Chicago
Xu, T., Rao, T., Yu, W., Ning, J., Yu, X., Zhu, S., Yang, K., Bai, T., Cheng, F."Upregulation of NFKBIZ affects bladder cancer progression via the PTEN/PI3K/Akt signaling pathway". International Journal of Molecular Medicine 47, no. 6 (2021): 109. https://doi.org/10.3892/ijmm.2021.4942