Spandidos Publications Logo
  • About
    • About Spandidos
    • Aims and Scopes
    • Abstracting and Indexing
    • Editorial Policies
    • Reprints and Permissions
    • Job Opportunities
    • Terms and Conditions
    • Contact
  • Journals
    • All Journals
    • Oncology Letters
      • Oncology Letters
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Oncology
      • International Journal of Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular and Clinical Oncology
      • Molecular and Clinical Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Experimental and Therapeutic Medicine
      • Experimental and Therapeutic Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Molecular Medicine
      • International Journal of Molecular Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Biomedical Reports
      • Biomedical Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Reports
      • Oncology Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular Medicine Reports
      • Molecular Medicine Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • World Academy of Sciences Journal
      • World Academy of Sciences Journal
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Functional Nutrition
      • International Journal of Functional Nutrition
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Epigenetics
      • International Journal of Epigenetics
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Medicine International
      • Medicine International
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
  • Articles
  • Information
    • Information for Authors
    • Information for Reviewers
    • Information for Librarians
    • Information for Advertisers
    • Conferences
  • Language Editing
Spandidos Publications Logo
  • About
    • About Spandidos
    • Aims and Scopes
    • Abstracting and Indexing
    • Editorial Policies
    • Reprints and Permissions
    • Job Opportunities
    • Terms and Conditions
    • Contact
  • Journals
    • All Journals
    • Biomedical Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Experimental and Therapeutic Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Epigenetics
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Functional Nutrition
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Molecular Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Medicine International
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular and Clinical Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular Medicine Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Letters
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • World Academy of Sciences Journal
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
  • Articles
  • Information
    • For Authors
    • For Reviewers
    • For Librarians
    • For Advertisers
    • Conferences
  • Language Editing
Login Register Submit
  • This site uses cookies
  • You can change your cookie settings at any time by following the instructions in our Cookie Policy. To find out more, you may read our Privacy Policy.

    I agree
Search articles by DOI, keyword, author or affiliation
Search
Advanced Search
presentation
International Journal of Molecular Medicine
Join Editorial Board Propose a Special Issue
Print ISSN: 1107-3756 Online ISSN: 1791-244X
Journal Cover
July-2021 Volume 48 Issue 1

Full Size Image

Sign up for eToc alerts
Recommend to Library

Journals

International Journal of Molecular Medicine

International Journal of Molecular Medicine

International Journal of Molecular Medicine is an international journal devoted to molecular mechanisms of human disease.

International Journal of Oncology

International Journal of Oncology

International Journal of Oncology is an international journal devoted to oncology research and cancer treatment.

Molecular Medicine Reports

Molecular Medicine Reports

Covers molecular medicine topics such as pharmacology, pathology, genetics, neuroscience, infectious diseases, molecular cardiology, and molecular surgery.

Oncology Reports

Oncology Reports

Oncology Reports is an international journal devoted to fundamental and applied research in Oncology.

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine is an international journal devoted to laboratory and clinical medicine.

Oncology Letters

Oncology Letters

Oncology Letters is an international journal devoted to Experimental and Clinical Oncology.

Biomedical Reports

Biomedical Reports

Explores a wide range of biological and medical fields, including pharmacology, genetics, microbiology, neuroscience, and molecular cardiology.

Molecular and Clinical Oncology

Molecular and Clinical Oncology

International journal addressing all aspects of oncology research, from tumorigenesis and oncogenes to chemotherapy and metastasis.

World Academy of Sciences Journal

World Academy of Sciences Journal

Multidisciplinary open-access journal spanning biochemistry, genetics, neuroscience, environmental health, and synthetic biology.

International Journal of Functional Nutrition

International Journal of Functional Nutrition

Open-access journal combining biochemistry, pharmacology, immunology, and genetics to advance health through functional nutrition.

International Journal of Epigenetics

International Journal of Epigenetics

Publishes open-access research on using epigenetics to advance understanding and treatment of human disease.

Medicine International

Medicine International

An International Open Access Journal Devoted to General Medicine.

Journal Cover
July-2021 Volume 48 Issue 1

Full Size Image

Sign up for eToc alerts
Recommend to Library

  • Article
  • Citations
    • Cite This Article
    • Download Citation
    • Create Citation Alert
    • Remove Citation Alert
    • Cited By
  • Similar Articles
    • Related Articles (in Spandidos Publications)
    • Similar Articles (Google Scholar)
    • Similar Articles (PubMed)
  • Download PDF
  • Download XML
  • View XML
Article Open Access

Myocardin‑related transcription factor A nuclear translocation contributes to mechanical overload‑induced nucleus pulposus fibrosis in rats with intervertebral disc degeneration

  • Authors:
    • Meng Kong
    • Yiran Zhang
    • Mengxiong Song
    • Wenbin Cong
    • Changtong Gao
    • Jiajun Zhang
    • Shuo Han
    • Qihao Tu
    • Xuexiao Ma
  • View Affiliations / Copyright

    Affiliations: Department of Spinal Surgery, The Affiliated Hospital of Qingdao University, Qing'dao, Shandong 266000, P.R. China, Department of Radiology, The Affiliated Hospital of Qingdao University, Qing'dao, Shandong 266000, P.R. China, Minimally Invasive Interventional Therapy Center, Qingdao Municipal Hospital, Qing'dao, Shandong 266000, P.R. China
    Copyright: © Kong et al. This is an open access article distributed under the terms of Creative Commons Attribution License [CC BY 4.0].
  • Article Number: 123
    |
    Published online on: May 10, 2021
       https://doi.org/10.3892/ijmm.2021.4956
  • Expand metrics +
Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )
Metrics: Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )
Cited By (CrossRef): 0 citations Loading Articles...

This article is mentioned in:



Abstract

Previous studies have reported that the Ras homolog family member A (RhoA)/myocardin‑related transcription factor A (MRTF‑A) nuclear translocation axis positively regulates fibrogenesis induced by mechanical forces in various organ systems. The aim of the present study was to determine whether this signaling pathway was involved in the pathogenesis of nucleus pulposus (NP) fibrosis induced by mechanical overload during the progression of intervertebral disc degeneration (IVDD) and to confirm the alleviating effect of an MRTF‑A inhibitor in the treatment of IVDD. NP cells (NPCs) were cultured on substrates of different stiffness (2.9 and 41.7 KPa), which mimicked normal and overloaded microenvironments, and were treated with an inhibitor of MRTF‑A nuclear import, CCG‑1423. In addition, bipedal rats were established by clipping the forelimbs of rats at 1 month and gradually elevating the feeding trough, and in order to establish a long‑term overload‑induced model of IVDD, and their intervertebral discs were injected with CCG‑1423 in situ. Cell viability was determined by Cell Counting Kit‑8 assay, and protein expression was determined by western blotting, immunofluorescence and immunohistochemical staining. The results demonstrated that the viability of NPCs was not affected by the application of force or the inhibitor. In NPCs cultured on stiff matrices, MRTF‑A was mostly localized in the nucleus, and the expression levels of fibrotic proteins, including type I collagen, connective tissue growth factor and α‑smooth muscle cell actin, were upregulated compared with those in NPCs cultured on soft matrices. The levels of these proteins were reduced by CCG‑1423 treatment. In rats, 6 months of upright posture activated MRTF‑A nuclear‑cytoplasmic trafficking and fibrogenesis in the NP and induced IVDD; these effects were alleviated by CCG‑1423 treatment. In conclusion, the results of the present study demonstrated that the RhoA/MRTF‑A translocation pathway may promote mechanical overload‑induced fibrogenic activity in NP tissue and partially elucidated the molecular mechanisms underlying the occurrence of IVDD.
View Figures

Figure 1

Figure 2

Figure 3

Figure 4

Figure 5

Figure 6

Figure 7

View References

1 

Fatoye F, Gebrye T and Odeyemi I: Real-world incidence and prevalence of low back pain using routinely collected data. Rheumatol Int. 39:619–626. 2019. View Article : Google Scholar : PubMed/NCBI

2 

GBD 2017 Disease and Injury Incidence and Prevalence Collaborators: Global, regional, and national incidence, prevalence, and years lived with disability for 354 diseases and injuries for 195 countries and territories, 1990-2017: A systematic analysis for the Global Burden of Disease Study 2017. Lancet. 392:1789–1858. 2018. View Article : Google Scholar

3 

Kleinman N, Patel AA, Benson C, Macario A, Kim M and Biondi DM: Economic burden of back and neck pain: Effect of a neuropathic component. Popul Health Manag. 17:224–232. 2014. View Article : Google Scholar : PubMed/NCBI

4 

Neidlinger-Wilke C, Galbusera F, Pratsinis H, Mavrogonatou E, Mietsch A, Kletsas D and Wilke HJ: Mechanical loading of the intervertebral disc: From the macroscopic to the cellular level. Eur Spine J. 23(Suppl 3): S333–S343. 2014. View Article : Google Scholar

5 

Setton LA and Chen J: Mechanobiology of the intervertebral disc and relevance to disc degeneration. J Bone Joint Surg Am. 88(Suppl 2): S52–S57. 2006.

6 

Pengb Y and Lv FJ: Fibrosis in intervertebral disc degeneration: Knowledge and gaps. Austin J Orthopade Rheumatol. 1:32014.

7 

Yee A, Lam MP, Tam V, Chan WC, Chu IK, Cheah KS, Cheung KM and Chan D: Fibrotic-like changes in degenerate human intervertebral discs revealed by quantitative proteomic analysis. Osteoarthritis Cartilage. 24:503–513. 2016. View Article : Google Scholar

8 

Nanthakumar CB, Hatley RJ, Lemma S, Gauldie J, Marshall RP and Macdonald SJ: Dissecting fibrosis: Therapeutic insights from the small-molecule toolbox. Nat Rev Drug Discov. 14:693–720. 2015. View Article : Google Scholar : PubMed/NCBI

9 

Olson EN and Nordheim A: Linking actin dynamics and gene transcription to drive cellular motile functions. Nat Rev Mol Cell Biol. 11:353–365. 2010. View Article : Google Scholar : PubMed/NCBI

10 

Marinissen MJ, Chiariello M, Tanos T, Bernard O, Narumiya S and Gutkind JS: The small GTP-binding protein RhoA regulates c-jun by a ROCK-JNK signaling axis. Mol Cell. 14:29–41. 2004. View Article : Google Scholar : PubMed/NCBI

11 

Liu F, Mih JD, Shea BS, Kho AT, Sharif AS, Tager AM and Tschumperlin DJ: Feedback amplification of fibrosis through matrix stiffening and COX-2 suppression. J Cell Biol. 190:693–706. 2010. View Article : Google Scholar : PubMed/NCBI

12 

Shimizu Y, Dobashi K, Iizuka K, Horie T, Suzuki K, Tukagoshi H, Nakazawa T, Nakazato Y and Mori M: Contribution of small GTPase Rho and its target protein rock in a murine model of lung fibrosis. Am J Respir Crit Care Med. 163:210–217. 2001. View Article : Google Scholar : PubMed/NCBI

13 

Satoh S, Ueda Y, Koyanagi M, Kadokami T, Sugano M, Yoshikawa Y and Makino N: Chronic inhibition of Rho kinase blunts the process of left ventricular hypertrophy leading to cardiac contractile dysfunction in hypertension-induced heart failure. J Mol Cell Cardiol. 35:59–70. 2003. View Article : Google Scholar : PubMed/NCBI

14 

Bei Y, Hua-Huy T, Nicco C, Duong-Quy S, Le-Dong NN, Tiev KP, Chéreau C, Batteux F and Dinh-Xuan AT: RhoA/Rho-kinase activation promotes lung fibrosis in an animal model of systemic sclerosis. Exp Lung Res. 42:44–55. 2016. View Article : Google Scholar : PubMed/NCBI

15 

Okumura N, Koizumi N, Ueno M, Sakamoto Y, Takahashi H, Hirata K, Torii R, Hamuro J and Kinoshita S: Enhancement of corneal endothelium wound healing by Rho-associated kinase (ROCK) inhibitor eye drops. Br J Ophthalmol. 95:1006–1009. 2011. View Article : Google Scholar : PubMed/NCBI

16 

Miralles F, Posern G, Zaromytidou AI and Treisman R: Actin dynamics control SRF activity by regulation of its coactivator MAL. Cell. 113:329–342. 2003. View Article : Google Scholar : PubMed/NCBI

17 

Hayashi K, Watanabe B, Nakagawa Y, Minami S and Morita T: RPEL proteins are the molecular targets for CCG-1423, an inhibitor of Rho signaling. PLoS One. 9:e890162014. View Article : Google Scholar : PubMed/NCBI

18 

Hiyama A, Arai F, Sakai D, Yokoyama K and Mochida J: The effects of oxygen tension and antiaging factor Klotho on Wnt signaling in nucleus pulposus cells. Arthritis Res Ther. 14:R1052012. View Article : Google Scholar : PubMed/NCBI

19 

Pelham RJ Jr and Wang Yl: Cell locomotion and focal adhesions are regulated by substrate flexibility. Proc Natl Acad Sci USA. 94:13661–13665. 1997. View Article : Google Scholar

20 

Tse JR and Engler AJ: Preparation of hydrogel substrates with tunable mechanical properties. Curr Protoc Cell Biol. 10:Unit 10.16. 2010.PubMed/NCBI

21 

Gilchrist CL, Darling EM, Chen J and Setton LA: Extracellular matrix ligand and stiffness modulate immature nucleus pulposus cell-cell interactions. PLoS One. 6:e271702011. View Article : Google Scholar : PubMed/NCBI

22 

Wallace MA, Della Gatta PA, Ahmad Mir B, Kowalski GM, Kloehn J, McConville MJ, Russell AP and Lamon S: Overexpression of striated muscle activator of Rho signaling (STARS) increases C2C12 skeletal muscle cell differentiation. Front Physiol. 7:72016. View Article : Google Scholar : PubMed/NCBI

23 

Esnault C, Stewart A, Gualdrini F, East P, Horswell S, Matthews N and Treisman R: Rho-actin signaling to the MRTF coactivators dominates the immediate transcriptional response to serum in fibroblasts. Genes Dev. 28:943–958. 2014. View Article : Google Scholar : PubMed/NCBI

24 

Xiao J, Qiu GX, Wu ZH, Xu J, Wang T, Wang YP and Weng XS: An improved operation approach for bipedal rat model construction. Zhonghua Yi Xue Za Zhi. 86:2781–2785. 2006.In Chinese.

25 

Liang X, Shen H, Shi WD, Ren S, Jiang W, Liu H, Yang P, Sun ZY, Lin J and Yang HL: Effect of axial vertical vibration on degeneration of lumbar intervertebral discs in modified bipedal rats: An in-vivo study. Asian Pac J Trop Med. 10:714–717. 2017. View Article : Google Scholar : PubMed/NCBI

26 

Thompson JP, Pearce RH, Schechter MT, Adams ME, Tsang IK and Bishop PB: Preliminary evaluation of a scheme for grading the gross morphology of the human intervertebral disc. Spine (Phila Pa 1976). 15:411–415. 1990. View Article : Google Scholar

27 

Ge J, Cheng X, Yuan C, Qian J, Wu C, Cao C, Yang H, Zhou F and Zou J: Syndecan-4 is a novel therapeutic target for intervertebral disc degeneration via suppressing JNK/p53 pathway. Int J Biol Sci. 16:766–776. 2020. View Article : Google Scholar : PubMed/NCBI

28 

Yang F, Leung VY, Luk KD, Chan D and Cheung KM: Injury-induced sequential transformation of notochordal nucleus pulposus to chondrogenic and fibrocartilaginous phenotype in the mouse. J Pathol. 218:113–121. 2009. View Article : Google Scholar : PubMed/NCBI

29 

Tam V, Chan WCW, Leung VYL, Cheah KSE, Cheung KMC, Sakai D, McCann MR, Bedore J, Séguin CA and Chan D: Histological and reference system for the analysis of mouse intervertebral disc. J Orthop Res. 36:233–243. 2018.

30 

Manohar M, Kandikattu HK, Verma AK and Mishra A: IL-15 regulates fibrosis and inflammation in a mouse model of chronic pancreatitis. Am J Physiol Gastrointest Liver Physiol. 315:G954–G965. 2018. View Article : Google Scholar : PubMed/NCBI

31 

Cui L, Wei H, Li ZM, Dong XB and Wang PY: TGF-β1 aggravates degenerative nucleus pulposus cells inflammation and fibrosis through the upregulation of angiopoietin-like protein 2 expression. Eur Rev Med Pharmacol Sci. 24:12025–12033. 2020.PubMed/NCBI

32 

Meng X, Zhuang L, Wang J, Liu Z, Wang Y, Xiao D and Zhang X: Hypoxia-inducible factor (HIF)-1alpha knockout accelerates intervertebral disc degeneration in mice. Int J Clin Exp Pathol. 11:548–557. 2018.PubMed/NCBI

33 

Eckes B, Nischt R and Krieg T: Cell-matrix interactions in dermal repair and scarring. Fibrogenesis Tissue Repair. 3:42010. View Article : Google Scholar : PubMed/NCBI

34 

Hinz B: The myofibroblast: Paradigm for a mechanically active cell. J Biomech. 43:146–155. 2010. View Article : Google Scholar

35 

Amano M, Nakayama M and Kaibuchi K: Rho-kinase/ROCK: A key regulator of the cytoskeleton and cell polarity. Cytoskeleton (Hoboken). 67:545–554. 2010. View Article : Google Scholar

36 

Bond JE, Kokosis G, Ren L, Selim MA, Bergeron A and Levinson H: Wound contraction is attenuated by fasudil inhibition of Rho-associated kinase. Plast Reconstr Surg. 128:438e–450e. 2011. View Article : Google Scholar : PubMed/NCBI

37 

Brown JH, Del Re DP and Sussman MA: The Rac and Rho hall of fame: A decade of hypertrophic signaling hits. Circ Res. 98:730–742. 2006. View Article : Google Scholar : PubMed/NCBI

38 

Holle AW and Engler AJ: More than a feeling: Discovering, understanding, and influencing mechanosensing pathways. Curr Opin Biotechnol. 22:648–654. 2011. View Article : Google Scholar : PubMed/NCBI

39 

Geneste O, Copeland JW and Treisman R: LIM kinase and Diaphanous cooperate to regulate serum response factor and actin dynamics. J Cell Biol. 157:831–838. 2002. View Article : Google Scholar : PubMed/NCBI

40 

Leask A, Parapuram SK, Shi-Wen X and Abraham DJ: Connective tissue growth factor (CTGF, CCN2) gene regulation: A potent clinical bio-marker of fibroproliferative disease? J Cell Commun Signal. 3:89–94. 2009. View Article : Google Scholar : PubMed/NCBI

41 

Blomme B, Deroanne C, Hulin A, Lambert C, Defraigne JO, Nusgens B, Radermecker M and Colige A: Mechanical strain induces a pro-fibrotic phenotype in human mitral valvular interstitial cells through RhoC/ROCK/MRTF-A and Erk1/2 signaling pathways. J Mol Cell Cardiol. 135:149–159. 2019. View Article : Google Scholar : PubMed/NCBI

42 

Johnson LA, Rodansky ES, Haak AJ, Larsen SD, Neubig RR and Higgins PD: Novel Rho/MRTF/SRF inhibitors block matrix-stiffness and TGF-β-induced fibrogenesis in human colonic myofibroblasts. Inflamm Bowel Dis. 20:154–165. 2014. View Article : Google Scholar

43 

Shiwen X, Stratton R, Nikitorowicz-Buniak J, Ahmed-Abdi B, Ponticos M, Denton C, Abraham D, Takahashi A, Suki B, Layne MD, et al: A role of myocardin related transcription factor-A (MRTF-A) in scleroderma related fibrosis. PLoS One. 10:e01260152015. View Article : Google Scholar : PubMed/NCBI

44 

Shao J, Xu H, Wu X and Xu Y: Epigenetic activation of CTGF transcription by high glucose in renal tubular epithelial cells is mediated by myocardin-related transcription factor A. Cell Tissue Res. 379:549–559. 2020. View Article : Google Scholar

45 

Arthur WT, Noren NK and Burridge K: Regulation of Rho family GTPases by cell-cell and cell-matrix adhesion. Biol Res. 35:239–246. 2002. View Article : Google Scholar : PubMed/NCBI

46 

Kim JG, Islam R, Cho JY, Jeong H, Cap KC, Park Y, Hossain AJ and Park JB: Regulation of RhoA GTPase and various transcription factors in the RhoA pathway. J Cell Physiol. 233:6381–6392. 2018. View Article : Google Scholar : PubMed/NCBI

47 

Takahara A, Sugiyama A, Satoh Y, Yoneyama M and Hashimoto K: Cardiovascular effects of Y-27632, a selective Rho-associated kinase inhibitor, assessed in the halothane-anesthetized canine model. Eur J Pharmacol. 460:51–57. 2003. View Article : Google Scholar : PubMed/NCBI

48 

Hinson JS, Medlin MD, Lockman K, Taylor JM and Mack CP: Smooth muscle cell-specific transcription is regulated by nuclear localization of the myocardin-related transcription factors. Am J Physiol Heart Circ Physiol. 292:H1170–1180. 2007. View Article : Google Scholar

49 

Pawłowski R, Rajakylä EK, Vartiainen MK and Treisman R: An actin-regulated importin α/β-dependent extended bipartite NLS directs nuclear import of MRTF-A. EMBO J. 29:3448–3458. 2010. View Article : Google Scholar

50 

Dai J, Qin L, Chen Y, Wang H, Lin G, Li X, Liao H and Fang H: Matrix stiffness regulates epithelial-mesenchymal transition via cytoskeletal remodeling and MRTF-A translocation in osteosarcoma cells. J Mech Behav Biomed Mater. 90:226–238. 2019. View Article : Google Scholar

51 

Hahmann C and Schroeter T: Rho-kinase inhibitors as therapeutics: From pan inhibition to isoform selectivity. Cell Mol Life Sci. 67:171–177. 2010. View Article : Google Scholar

52 

Nakamura S, Hayashi K, Iwasaki K, Fujioka T, Egusa H, Yatani H and Sobue K: Nuclear import mechanism for myocardin family members and their correlation with vascular smooth muscle cell phenotype. J Biol Chem. 285:37314–37323. 2010. View Article : Google Scholar : PubMed/NCBI

53 

Zhang YH, Zhao CQ, Jiang LS and Dai LY: Substrate stiffness regulates apoptosis and the mRNA expression of extracellular matrix regulatory genes in the rat annular cells. Matrix Biol. 30:135–144. 2011. View Article : Google Scholar

54 

Hoffman BD, Grashoff C and Schwartz MA: Dynamic molecular processes mediate cellular mechanotransduction. Nature. 475:316–323. 2011. View Article : Google Scholar : PubMed/NCBI

55 

Waxenbaum JA, Reddy V and Futterman B: Anatomy, Back, Intervertebral Discs. StatPearls. StatPearls Publishing. Copyright© 2020, StatPearls Publishing LLC; Treasure Island, FL: 2020

56 

Sivan SS, Roberts S, Urban JP, Menage J, Bramhill J, Campbell D, Franklin VJ, Lydon F, Merkher Y, Maroudas A and Tighe BJ: Injectable hydrogels with high fixed charge density and swelling pressure for nucleus pulposus repair: Biomimetic glycosaminoglycan analogues. Acta Biomater. 10:1124–1133. 2014. View Article : Google Scholar

57 

Chelberg MK, Banks GM, Geiger DF and Oegema TR Jr: Identification of heterogeneous cell populations in normal human intervertebral disc. J Anat. 186:43–53. 1995.PubMed/NCBI

58 

Zhang YG, Sun ZM, Liu JT, Wang SJ, Ren FL and Guo X: Features of intervertebral disc degeneration in rat's aging process. J Zhejiang Univ Sci B. 10:522–527. 2009. View Article : Google Scholar : PubMed/NCBI

59 

Huang X, Yang N, Fiore VF, Barker TH, Sun Y, Morris SW, Ding Q, Thannickal VJ and Zhou Y: Matrix stiffness-induced myofibroblast differentiation is mediated by intrinsic mechanotransduction. Am J Respir Cell Mol Biol. 47:340–348. 2012. View Article : Google Scholar : PubMed/NCBI

60 

Zhao XH, Laschinger C, Arora P, Szászi K, Kapus A and McCulloch CA: Force activates smooth muscle alpha-actin promoter activity through the Rho signaling pathway. Cell Sci. 120:1801–1809. 2007. View Article : Google Scholar

61 

Johnson LA, Rodansky ES, Sauder KL, Horowitz JC, Mih JD, Tschumperlin DJ and Higgins PD: Matrix stiffness corresponding to strictured bowel induces a fibrogenic response in human colonic fibroblasts. Inflamm Bowel Dis. 19:891–903. 2013. View Article : Google Scholar : PubMed/NCBI

62 

Zheng L, Qin J, Sun L, Gui L, Zhang C, Huang Y, Deng W, Huang A, Sun D and Luo M: Intrahepatic upregulation of MRTF-A signaling contributes to increased hepatic vascular resistance in cirrhotic rats with portal hypertension. Clin Res Hepatol Gastroenterol. 41:303–310. 2017. View Article : Google Scholar : PubMed/NCBI

63 

Hyväri L, Vanhatupa S, Halonen HT, Kääriäinen M and Miettinen S: Myocardin-related transcription factor A (MRTF-A) regulates the balance between adipogenesis and osteogenesis of human adipose stem cells. Stem Cells Int. 2020:88535412020. View Article : Google Scholar : PubMed/NCBI

Related Articles

  • Abstract
  • View
  • Download
  • Twitter
Copy and paste a formatted citation
Spandidos Publications style
Kong M, Zhang Y, Song M, Cong W, Gao C, Zhang J, Han S, Tu Q and Ma X: Myocardin‑related transcription factor A nuclear translocation contributes to mechanical overload‑induced nucleus pulposus fibrosis in rats with intervertebral disc degeneration. Int J Mol Med 48: 123, 2021.
APA
Kong, M., Zhang, Y., Song, M., Cong, W., Gao, C., Zhang, J. ... Ma, X. (2021). Myocardin‑related transcription factor A nuclear translocation contributes to mechanical overload‑induced nucleus pulposus fibrosis in rats with intervertebral disc degeneration. International Journal of Molecular Medicine, 48, 123. https://doi.org/10.3892/ijmm.2021.4956
MLA
Kong, M., Zhang, Y., Song, M., Cong, W., Gao, C., Zhang, J., Han, S., Tu, Q., Ma, X."Myocardin‑related transcription factor A nuclear translocation contributes to mechanical overload‑induced nucleus pulposus fibrosis in rats with intervertebral disc degeneration". International Journal of Molecular Medicine 48.1 (2021): 123.
Chicago
Kong, M., Zhang, Y., Song, M., Cong, W., Gao, C., Zhang, J., Han, S., Tu, Q., Ma, X."Myocardin‑related transcription factor A nuclear translocation contributes to mechanical overload‑induced nucleus pulposus fibrosis in rats with intervertebral disc degeneration". International Journal of Molecular Medicine 48, no. 1 (2021): 123. https://doi.org/10.3892/ijmm.2021.4956
Copy and paste a formatted citation
x
Spandidos Publications style
Kong M, Zhang Y, Song M, Cong W, Gao C, Zhang J, Han S, Tu Q and Ma X: Myocardin‑related transcription factor A nuclear translocation contributes to mechanical overload‑induced nucleus pulposus fibrosis in rats with intervertebral disc degeneration. Int J Mol Med 48: 123, 2021.
APA
Kong, M., Zhang, Y., Song, M., Cong, W., Gao, C., Zhang, J. ... Ma, X. (2021). Myocardin‑related transcription factor A nuclear translocation contributes to mechanical overload‑induced nucleus pulposus fibrosis in rats with intervertebral disc degeneration. International Journal of Molecular Medicine, 48, 123. https://doi.org/10.3892/ijmm.2021.4956
MLA
Kong, M., Zhang, Y., Song, M., Cong, W., Gao, C., Zhang, J., Han, S., Tu, Q., Ma, X."Myocardin‑related transcription factor A nuclear translocation contributes to mechanical overload‑induced nucleus pulposus fibrosis in rats with intervertebral disc degeneration". International Journal of Molecular Medicine 48.1 (2021): 123.
Chicago
Kong, M., Zhang, Y., Song, M., Cong, W., Gao, C., Zhang, J., Han, S., Tu, Q., Ma, X."Myocardin‑related transcription factor A nuclear translocation contributes to mechanical overload‑induced nucleus pulposus fibrosis in rats with intervertebral disc degeneration". International Journal of Molecular Medicine 48, no. 1 (2021): 123. https://doi.org/10.3892/ijmm.2021.4956
Follow us
  • Twitter
  • LinkedIn
  • Facebook
About
  • Spandidos Publications
  • Careers
  • Cookie Policy
  • Privacy Policy
How can we help?
  • Help
  • Live Chat
  • Contact
  • Email to our Support Team