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Omega‑3 polyunsaturated fatty acids inhibit IL‑11/STAT3 signaling in hepatocytes during acetaminophen hepatotoxicity

  • Authors:
    • Yunzhi Liu
    • Jingmin Lin
    • Yu Chen
    • Zhuonan Li
    • Jia Zhou
    • Xiao Lu
    • Zhengliang Chen
    • Daming Zuo
  • View Affiliations / Copyright

    Affiliations: Department of Medical Laboratory, School of Laboratory Medicine and Biotechnology, Southern Medical University, Guangzhou, Guangdong 510515, P.R. China, Guangdong Province Key Laboratory of Proteomics, Department of Immunology, School of Basic Medical Sciences, Southern Medical University, Guangzhou, Guangdong 510515, P.R. China, College of Marine Life Sciences, Ocean University of China, Qingdao, Shandong 266003, P.R. China
    Copyright: © Liu et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Article Number: 190
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    Published online on: August 17, 2021
       https://doi.org/10.3892/ijmm.2021.5023
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Abstract

Omega‑3 polyunsaturated fatty acids (n‑3 PUFAs) exert a negative effect on IL‑6 production in several liver disorders, including cirrhosis, acute liver failure and fatty liver disease. However, its effect on the production of IL‑11, another important IL‑6 family cytokine, remains unclear. IL‑11 was found to be significantly elevated in acetaminophen (APAP)‑induced liver damage. The aim of the present study was to investigate whether and how n‑3 PUFAs modulate IL‑11 production during APAP‑induced liver injury. For that purpose, wild‑type (WT) and fat‑1 transgenic mice were intraperitoneally injected with APAP to induce liver injury. Serum was collected for ELISA and alanine aminotransferase assay. The hepatocytes of APAP‑injected mice were isolated for reverse transcription‑quantitative PCR and western blot analyses. For the in vitro study, primary hepatocytes isolated from WT or fat‑1 mice were stimulated with APAP. The results revealed that both endogenous and exogenous n‑3 PUFAs significantly aggravated APAP‑induced liver damage via the downregulation of STAT3 signaling. Notably, n‑3 PUFAs inhibited IL‑11 expression, but not IL‑6 expression in hepatocytes during the APAP challenge. Furthermore, it was demonstrated that limited phosphorylation of ERK1/2 and Fos‑​like‑1 (Fra‑1) expression are responsible for the n‑3 PUFA‑mediated inhibitory effect on IL‑11 production in APAP‑treated hepatocytes. It was concluded that n‑3 PUFAs inhibit IL‑11 production and further STAT3 activation in hepatocytes during APAP‑induced liver injury. Therefore, ERK1/2‑mediated Fra‑1 expression is responsible for the effect of n‑3 PUFAs on IL‑11 expression.
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Copy and paste a formatted citation
Spandidos Publications style
Liu Y, Lin J, Chen Y, Li Z, Zhou J, Lu X, Chen Z and Zuo D: Omega‑3 polyunsaturated fatty acids inhibit IL‑11/STAT3 signaling in hepatocytes during acetaminophen hepatotoxicity. Int J Mol Med 48: 190, 2021.
APA
Liu, Y., Lin, J., Chen, Y., Li, Z., Zhou, J., Lu, X. ... Zuo, D. (2021). Omega‑3 polyunsaturated fatty acids inhibit IL‑11/STAT3 signaling in hepatocytes during acetaminophen hepatotoxicity. International Journal of Molecular Medicine, 48, 190. https://doi.org/10.3892/ijmm.2021.5023
MLA
Liu, Y., Lin, J., Chen, Y., Li, Z., Zhou, J., Lu, X., Chen, Z., Zuo, D."Omega‑3 polyunsaturated fatty acids inhibit IL‑11/STAT3 signaling in hepatocytes during acetaminophen hepatotoxicity". International Journal of Molecular Medicine 48.4 (2021): 190.
Chicago
Liu, Y., Lin, J., Chen, Y., Li, Z., Zhou, J., Lu, X., Chen, Z., Zuo, D."Omega‑3 polyunsaturated fatty acids inhibit IL‑11/STAT3 signaling in hepatocytes during acetaminophen hepatotoxicity". International Journal of Molecular Medicine 48, no. 4 (2021): 190. https://doi.org/10.3892/ijmm.2021.5023
Copy and paste a formatted citation
x
Spandidos Publications style
Liu Y, Lin J, Chen Y, Li Z, Zhou J, Lu X, Chen Z and Zuo D: Omega‑3 polyunsaturated fatty acids inhibit IL‑11/STAT3 signaling in hepatocytes during acetaminophen hepatotoxicity. Int J Mol Med 48: 190, 2021.
APA
Liu, Y., Lin, J., Chen, Y., Li, Z., Zhou, J., Lu, X. ... Zuo, D. (2021). Omega‑3 polyunsaturated fatty acids inhibit IL‑11/STAT3 signaling in hepatocytes during acetaminophen hepatotoxicity. International Journal of Molecular Medicine, 48, 190. https://doi.org/10.3892/ijmm.2021.5023
MLA
Liu, Y., Lin, J., Chen, Y., Li, Z., Zhou, J., Lu, X., Chen, Z., Zuo, D."Omega‑3 polyunsaturated fatty acids inhibit IL‑11/STAT3 signaling in hepatocytes during acetaminophen hepatotoxicity". International Journal of Molecular Medicine 48.4 (2021): 190.
Chicago
Liu, Y., Lin, J., Chen, Y., Li, Z., Zhou, J., Lu, X., Chen, Z., Zuo, D."Omega‑3 polyunsaturated fatty acids inhibit IL‑11/STAT3 signaling in hepatocytes during acetaminophen hepatotoxicity". International Journal of Molecular Medicine 48, no. 4 (2021): 190. https://doi.org/10.3892/ijmm.2021.5023
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