Open Access

Ailanthone suppresses the activity of human colorectal cancer cells through the STAT3 signaling pathway

  • Authors:
    • Haixiang Ding
    • Xiuchong Yu
    • Zhilong Yan
  • View Affiliations

  • Published online on: December 27, 2021     https://doi.org/10.3892/ijmm.2021.5076
  • Article Number: 21
  • Copyright: © Ding et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

Ailanthone (AIL) is a major quassinoid extracted from the Chinese medicinal herb, Ailanthus altissima, which has been reported to exert anti‑proliferative effects on various cancer cells. The present study aimed to investigate the antitumor effects of AIL on HCT116 and SW620 colon cancer cells, and to analyze the underlying molecular mechanisms. CCK‑8 assay was used to detect cell viability. Furthermore, colony formation and Transwell assays, and flow cytometry were used to examine the effects of AIL on cell proliferation, apoptosis and migration. Finally, the expression levels of cell cycle control proteins, and caspase and Bcl‑2 family‑related proteins involved in the regulation of apoptosis, as well as those of cell migration‑ and pathway‑related proteins were examined using western blot analysis. Reverse transcription‑quantitative PCR was used to quantitatively analyze the changes in the JAK and STAT3 gene levels in each group. The in vitro cell function tests revealed that AIL inhibited the proliferation and migration, and induced the apoptosis and cell cycle arrest of HCT116 and SW620 cells. It was further found exerted these effects via the JAK/STAT3 signaling pathway, as well as through caspase and Bcl‑2 family proteins. On the whole, the present study demonstrates that AIL suppresses the activity of colon cancer cells via the STAT3 pathway.
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February-2022
Volume 49 Issue 2

Print ISSN: 1107-3756
Online ISSN:1791-244X

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Spandidos Publications style
Ding H, Yu X and Yan Z: Ailanthone suppresses the activity of human colorectal cancer cells through the STAT3 signaling pathway. Int J Mol Med 49: 21, 2022
APA
Ding, H., Yu, X., & Yan, Z. (2022). Ailanthone suppresses the activity of human colorectal cancer cells through the STAT3 signaling pathway. International Journal of Molecular Medicine, 49, 21. https://doi.org/10.3892/ijmm.2021.5076
MLA
Ding, H., Yu, X., Yan, Z."Ailanthone suppresses the activity of human colorectal cancer cells through the STAT3 signaling pathway". International Journal of Molecular Medicine 49.2 (2022): 21.
Chicago
Ding, H., Yu, X., Yan, Z."Ailanthone suppresses the activity of human colorectal cancer cells through the STAT3 signaling pathway". International Journal of Molecular Medicine 49, no. 2 (2022): 21. https://doi.org/10.3892/ijmm.2021.5076