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Potential mechanisms of osteoprotegerin-induced damage to osteoclast adhesion structures via P2X7R-mediated MAPK signaling

  • Authors:
    • Yonggang Ma
    • Xueni Shi
    • Hongyan Zhao
    • Ruilong Song
    • Hui Zou
    • Jiaqiao Zhu
    • Zongping Liu
  • View Affiliations / Copyright

    Affiliations: College of Veterinary Medicine, Yangzhou University, Yangzhou, Jiangsu 225009, P.R. China, College of Veterinary Medicine, Yangzhou University, Yangzhou, Jiangsu 225009, P.R. China
    Copyright: © Ma et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Article Number: 59
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    Published online on: March 9, 2022
       https://doi.org/10.3892/ijmm.2022.5115
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Abstract

Osteoprotegerin (OPG) is a negative regulator of osteoclast formation by competing with receptor activator of the nuclear factor-κB (NF-κB) ligand (RANKL) for RANK. OPG is not only a soluble decoy receptor for RANKL, but is also considered as a direct effector of osteoclast functions. However, the mechanismsresponsible for OPG-induced changes to osteoclast bone resorption functionsremain unknown. P2X7R is involved in the process of multinucleation and cell fusion. Therefore, in the present study, mitogen-activated protein kinase (MAPK) inhibitors and the RNA interference of purinergic receptor P2X7 (P2X7R) were usedtoexamine the effects of P2X7R-mediated MAPK signaling on changes to osteoclast adhesion structure induced by OPG; for this purpose, western blot analysis and immunofluorescence staining were performed. The results revealed that OPG inhibited osteoclast adhesion-related protein expression, disrupted adhesion protein distribution, and destroyed osteoclast filopodia and lamellipodia structures. The inhibitors partially restored osteoclast adhesion structure, including protein expression, distribution and cell morphology. The absence of P2X7R markedly inhibited osteoclast formation, and subsequent OPG treatment accelerated the damage to adhesion structures. However, P2X7R activation significantly recosvered the phosphorylation of paxillin, vinculin, phosphorylated protein tyrosine kinase 2 and SRC proto-oncogene, non-receptor tyrosine kinase induced by OPG, and their distribution was uniform at the osteoclast periphery. P2X7R silencing suppressed the phosphorylation of MAPK. On the whole, the findings of the present study highlighta key role of P2X7R/MAPK signaling in osteoclast adhesion, and provide a novel therapeutic target for bone disease.
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Copy and paste a formatted citation
Spandidos Publications style
Ma Y, Shi X, Zhao H, Song R, Zou H, Zhu J and Liu Z: Potential mechanisms of osteoprotegerin-induced damage to osteoclast adhesion structures via P2X7R-mediated MAPK signaling. Int J Mol Med 49: 59, 2022.
APA
Ma, Y., Shi, X., Zhao, H., Song, R., Zou, H., Zhu, J., & Liu, Z. (2022). Potential mechanisms of osteoprotegerin-induced damage to osteoclast adhesion structures via P2X7R-mediated MAPK signaling. International Journal of Molecular Medicine, 49, 59. https://doi.org/10.3892/ijmm.2022.5115
MLA
Ma, Y., Shi, X., Zhao, H., Song, R., Zou, H., Zhu, J., Liu, Z."Potential mechanisms of osteoprotegerin-induced damage to osteoclast adhesion structures via P2X7R-mediated MAPK signaling". International Journal of Molecular Medicine 49.5 (2022): 59.
Chicago
Ma, Y., Shi, X., Zhao, H., Song, R., Zou, H., Zhu, J., Liu, Z."Potential mechanisms of osteoprotegerin-induced damage to osteoclast adhesion structures via P2X7R-mediated MAPK signaling". International Journal of Molecular Medicine 49, no. 5 (2022): 59. https://doi.org/10.3892/ijmm.2022.5115
Copy and paste a formatted citation
x
Spandidos Publications style
Ma Y, Shi X, Zhao H, Song R, Zou H, Zhu J and Liu Z: Potential mechanisms of osteoprotegerin-induced damage to osteoclast adhesion structures via P2X7R-mediated MAPK signaling. Int J Mol Med 49: 59, 2022.
APA
Ma, Y., Shi, X., Zhao, H., Song, R., Zou, H., Zhu, J., & Liu, Z. (2022). Potential mechanisms of osteoprotegerin-induced damage to osteoclast adhesion structures via P2X7R-mediated MAPK signaling. International Journal of Molecular Medicine, 49, 59. https://doi.org/10.3892/ijmm.2022.5115
MLA
Ma, Y., Shi, X., Zhao, H., Song, R., Zou, H., Zhu, J., Liu, Z."Potential mechanisms of osteoprotegerin-induced damage to osteoclast adhesion structures via P2X7R-mediated MAPK signaling". International Journal of Molecular Medicine 49.5 (2022): 59.
Chicago
Ma, Y., Shi, X., Zhao, H., Song, R., Zou, H., Zhu, J., Liu, Z."Potential mechanisms of osteoprotegerin-induced damage to osteoclast adhesion structures via P2X7R-mediated MAPK signaling". International Journal of Molecular Medicine 49, no. 5 (2022): 59. https://doi.org/10.3892/ijmm.2022.5115
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