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Article Open Access

Identification of novel molecules and pathways associated with fascin actin‑bundling protein 1 in laryngeal squamous cell carcinoma through comprehensive transcriptome analysis

  • Authors:
    • Hongliang Liu
    • Wenjing Hao
    • Xinfang Wang
    • Yuliang Zhang
    • Long He
    • Xuting Xue
    • Jiao Yang
    • Chunming Zhang
  • View Affiliations / Copyright

    Affiliations: Shanxi Key Laboratory of Otorhinolaryngology Head and Neck Cancer, First Hospital of Shanxi Medical University, Taiyuan, Shanxi 030001, P.R. China, Department of Anatomy, the Basic Medical School of Shanxi Medical University, Taiyuan, Shanxi 030001, P.R. China
    Copyright: © Liu et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Article Number: 39
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    Published online on: February 28, 2024
       https://doi.org/10.3892/ijmm.2024.5363
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Abstract

Laryngeal squamous cell carcinoma (LSCC) is a common malignant tumor with a poor prognosis. Fascin actin‑bundling protein 1 (FSCN1) has been reported to play a crucial role in the development and progression of LSCC; however, the underlying molecular mechanisms remain unknown. Herein, a whole transcriptome microarray analysis was performed to screen for differentially expressed genes (DEGs) in cells in which FSCN1 was knocked down. A total of 462 up and 601 downregulated mRNA transcripts were identified. Functional annotation analysis revealed that these DEGs were involved in multiple biological functions, such as transcriptional regulation, response to radiation, focal adhesion, extracellular matrix‑receptor interaction, steroid biosynthesis and others. Through co‑expression and protein‑protein interaction analysis, FSCN1 was linked to novel functions, including defense response to virus and steroid biosynthesis. Furthermore, crosstalk analysis with FSCN1‑interacting proteins revealed seven DEGs, identified as FSCN1‑interacting partners, in LSCC cells, three of which were selected for further validation. Co‑immunoprecipitation validation confirmed that FSCN1 interacted with prostaglandin reductase 1 and 24‑dehydrocholesterol reductase (DHCR24). Of note, DHCR24 is a key enzyme involved in cholesterol biosynthesis, and its overexpression promotes the proliferation and migration of LSCC cells. These findings suggest that DHCR24 is a novel molecule associated with FSCN1 in LSCC, and that the FSCN1‑DHCR24 interaction may promote LSCC progression by regulating cholesterol metabolism‑related signaling pathways.
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Copy and paste a formatted citation
Spandidos Publications style
Liu H, Hao W, Wang X, Zhang Y, He L, Xue X, Yang J and Zhang C: Identification of novel molecules and pathways associated with fascin actin‑bundling protein 1 in laryngeal squamous cell carcinoma through comprehensive transcriptome analysis. Int J Mol Med 53: 39, 2024.
APA
Liu, H., Hao, W., Wang, X., Zhang, Y., He, L., Xue, X. ... Zhang, C. (2024). Identification of novel molecules and pathways associated with fascin actin‑bundling protein 1 in laryngeal squamous cell carcinoma through comprehensive transcriptome analysis. International Journal of Molecular Medicine, 53, 39. https://doi.org/10.3892/ijmm.2024.5363
MLA
Liu, H., Hao, W., Wang, X., Zhang, Y., He, L., Xue, X., Yang, J., Zhang, C."Identification of novel molecules and pathways associated with fascin actin‑bundling protein 1 in laryngeal squamous cell carcinoma through comprehensive transcriptome analysis". International Journal of Molecular Medicine 53.4 (2024): 39.
Chicago
Liu, H., Hao, W., Wang, X., Zhang, Y., He, L., Xue, X., Yang, J., Zhang, C."Identification of novel molecules and pathways associated with fascin actin‑bundling protein 1 in laryngeal squamous cell carcinoma through comprehensive transcriptome analysis". International Journal of Molecular Medicine 53, no. 4 (2024): 39. https://doi.org/10.3892/ijmm.2024.5363
Copy and paste a formatted citation
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Spandidos Publications style
Liu H, Hao W, Wang X, Zhang Y, He L, Xue X, Yang J and Zhang C: Identification of novel molecules and pathways associated with fascin actin‑bundling protein 1 in laryngeal squamous cell carcinoma through comprehensive transcriptome analysis. Int J Mol Med 53: 39, 2024.
APA
Liu, H., Hao, W., Wang, X., Zhang, Y., He, L., Xue, X. ... Zhang, C. (2024). Identification of novel molecules and pathways associated with fascin actin‑bundling protein 1 in laryngeal squamous cell carcinoma through comprehensive transcriptome analysis. International Journal of Molecular Medicine, 53, 39. https://doi.org/10.3892/ijmm.2024.5363
MLA
Liu, H., Hao, W., Wang, X., Zhang, Y., He, L., Xue, X., Yang, J., Zhang, C."Identification of novel molecules and pathways associated with fascin actin‑bundling protein 1 in laryngeal squamous cell carcinoma through comprehensive transcriptome analysis". International Journal of Molecular Medicine 53.4 (2024): 39.
Chicago
Liu, H., Hao, W., Wang, X., Zhang, Y., He, L., Xue, X., Yang, J., Zhang, C."Identification of novel molecules and pathways associated with fascin actin‑bundling protein 1 in laryngeal squamous cell carcinoma through comprehensive transcriptome analysis". International Journal of Molecular Medicine 53, no. 4 (2024): 39. https://doi.org/10.3892/ijmm.2024.5363
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