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Liriodendrin alleviates myocardial ischemia‑reperfusion injury via partially attenuating apoptosis, inflammation and mitochondria damage in rats

  • Authors:
    • Bo Li
    • Wei-Wei Yang
    • Bo-Chen Yao
    • Qing-Liang Chen
    • Li-Li Zhao
    • Yan-Qiu Song
    • Nan Jiang
    • Zhi-Gang Guo
  • View Affiliations / Copyright

    Affiliations: Department of Intensive Care, Tianjin Chest Hospital, Tianjin 300001, P.R. China, Department of Clinical Laboratory, Tianjin Central Hospital of Gynecology Obstetrics, Nankai University Maternity Hospital, Tianjin 300100, P.R. China, Department of Cardiovascular Surgery, Tianjin Chest Hospital, Tianjin 300001, P.R. China, Tianjin Institute of Cardiovascular Diseases, Tianjin 300001, P.R. China
    Copyright: © Li et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Article Number: 65
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    Published online on: February 20, 2025
       https://doi.org/10.3892/ijmm.2025.5506
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Abstract

Myocardial ischemia‑reperfusion (I/R) injury may lead to dysfunction of signaling pathways related to cell apoptosis, inflammation, oxidative stress, and mitochondrial damage. The present study investigated the defensive effect of liriodendrin, as a natural product isolated from Linaria vulgaris, on reperfusion injury in rats and the underlying mechanisms involved in this process. An in vivo rat model of I/R constructed by ligation of the left anterior descending artery, as well as an in vitro model using H9C2 cells under hypoxic conditions, was established to assess the cardioprotective effects of liriodendrin. The biomarkers of myocardial damage, oxidative stress, and inflammatory response were measured with enzyme‑linked immunosorbent assay (ELISA). Gene and protein expression were detected by reverse transcription‑quantitative PCR (RT‑qPCR) and western blotting. Mitochondrial morphology was observed by electron microscopy. The levels of creatine kinase isoenzymes and cardiac troponin T were significantly elevated in the I/R compared with the sham group; liriodendrin mitigated this elevation. The liriodendrin group exhibited a significant reduction in myocardial tissue apoptosis, as indicated by immunohistochemical staining and western blotting. Additionally, ELISA indicated that the I/R group had higher levels of reactive oxygen species (ROS) compared with the liriodendrin group, while the liriodendrin group had higher levels of superoxide dismutase. The in vitro experiments demonstrated that liriodendrin ameliorated hypoxia‑induced injury to mitochondria and suppressed the activation of nuclear factor-κB and B-cell lymphoma-2 associated X protein (Bax). Therefore, the present study demonstrated that liriodendrin impeded ROS‑associated metabolic disorders, maintained mitochondrial homeostasis and partially alleviated cardiomyocyte apoptosis by inhibiting the Bax signaling pathway.
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Copy and paste a formatted citation
Spandidos Publications style
Li B, Yang W, Yao B, Chen Q, Zhao L, Song Y, Jiang N and Guo Z: Liriodendrin alleviates myocardial ischemia‑reperfusion injury via partially attenuating apoptosis, inflammation and mitochondria damage in rats. Int J Mol Med 55: 65, 2025.
APA
Li, B., Yang, W., Yao, B., Chen, Q., Zhao, L., Song, Y. ... Guo, Z. (2025). Liriodendrin alleviates myocardial ischemia‑reperfusion injury via partially attenuating apoptosis, inflammation and mitochondria damage in rats. International Journal of Molecular Medicine, 55, 65. https://doi.org/10.3892/ijmm.2025.5506
MLA
Li, B., Yang, W., Yao, B., Chen, Q., Zhao, L., Song, Y., Jiang, N., Guo, Z."Liriodendrin alleviates myocardial ischemia‑reperfusion injury via partially attenuating apoptosis, inflammation and mitochondria damage in rats". International Journal of Molecular Medicine 55.4 (2025): 65.
Chicago
Li, B., Yang, W., Yao, B., Chen, Q., Zhao, L., Song, Y., Jiang, N., Guo, Z."Liriodendrin alleviates myocardial ischemia‑reperfusion injury via partially attenuating apoptosis, inflammation and mitochondria damage in rats". International Journal of Molecular Medicine 55, no. 4 (2025): 65. https://doi.org/10.3892/ijmm.2025.5506
Copy and paste a formatted citation
x
Spandidos Publications style
Li B, Yang W, Yao B, Chen Q, Zhao L, Song Y, Jiang N and Guo Z: Liriodendrin alleviates myocardial ischemia‑reperfusion injury via partially attenuating apoptosis, inflammation and mitochondria damage in rats. Int J Mol Med 55: 65, 2025.
APA
Li, B., Yang, W., Yao, B., Chen, Q., Zhao, L., Song, Y. ... Guo, Z. (2025). Liriodendrin alleviates myocardial ischemia‑reperfusion injury via partially attenuating apoptosis, inflammation and mitochondria damage in rats. International Journal of Molecular Medicine, 55, 65. https://doi.org/10.3892/ijmm.2025.5506
MLA
Li, B., Yang, W., Yao, B., Chen, Q., Zhao, L., Song, Y., Jiang, N., Guo, Z."Liriodendrin alleviates myocardial ischemia‑reperfusion injury via partially attenuating apoptosis, inflammation and mitochondria damage in rats". International Journal of Molecular Medicine 55.4 (2025): 65.
Chicago
Li, B., Yang, W., Yao, B., Chen, Q., Zhao, L., Song, Y., Jiang, N., Guo, Z."Liriodendrin alleviates myocardial ischemia‑reperfusion injury via partially attenuating apoptosis, inflammation and mitochondria damage in rats". International Journal of Molecular Medicine 55, no. 4 (2025): 65. https://doi.org/10.3892/ijmm.2025.5506
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