Immunoglobulin heavy chain gene analysis in bone marrow biopsies and corresponding lymph node specimens: Dependency on pre-treatment, histological subtype and extension of B-cell lymphoma

Odenthal,Margarete; Siebolts,Udo; Ernestus,Karen; Disse,Daniel; Dienes,Hans Peter; Wickenhauser,Claudia

doi:10.3892/ijmm.21.5.569

May 2008 Volume 21 Issue 5

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May 2008 Volume 21 Issue 5

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Article

Immunoglobulin heavy chain gene analysis in bone marrow biopsies and corresponding lymph node specimens: Dependency on pre-treatment, histological subtype and extension of B-cell lymphoma

Authors:
- Margarete Odenthal
- Udo Siebolts
- Karen Ernestus
- Daniel Disse
- Hans Peter Dienes
- Claudia Wickenhauser
View Affiliations / Copyright

Affiliations: Institute of Pathology, University of Cologne, D-50924 Cologne, Germany
Pages: 569-576
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Published online on: May 1, 2008

https://doi.org/10.3892/ijmm.21.5.569
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Abstract

Bone marrow biopsies (BMB) are the conventional staging method for assessing marrow involvement by lymphoma. Morphological criteria provide basic data determining their dignity, but concerning microfocal infiltrates, these criteria are rather inaccurate. Here, by examination of immunoglobulin H (IgH) receptor rearrangement and comparison of medullar and nodular lymphoma sites, diagnostic reliability was improved. Employing non-nested IgH rearrangement analysis with FR3A and JHa consensus primers, B-cell clonality was assessed on glutardialdehyde fixed, decalcified BMB with and without lymphoma infiltration and on the corresponding lymph node specimens. Malignancy was confirmed when polymerase chain reaction (PCR) generated no more than two peaks and was observed in 60% of the medullar B-cell lymphoma. Comparison of lymph node tissues and BMB revealed an identical pattern in 50% of the probes. In 25% of the cases a single clonal peak derived from the lymph node tissues was also observed in the BMB but was surrounded by additional peaks. Here, direct comparison of the data permitted determination of lymphoma in the BMB. Therefore, IgH FR3 PCR analysis is a suitable tool to examine small lymphoid infiltrates in BMB, and direct comparison with corresponding nodal lymphoma can further facilitate estimation of their dignity.

Journals

International Journal of Molecular Medicine

International Journal of Oncology

Molecular Medicine Reports

Oncology Reports

Experimental and Therapeutic Medicine

Oncology Letters

Biomedical Reports

Molecular and Clinical Oncology

World Academy of Sciences Journal

International Journal of Functional Nutrition

International Journal of Epigenetics

Medicine International

Immunoglobulin heavy chain gene analysis in bone marrow biopsies and corresponding lymph node specimens: Dependency on pre-treatment, histological subtype and extension of B-cell lymphoma

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Abstract

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