Modulation of TNF-α-induced endothelial cell activation by glucosamine, a naturally occurring amino monosaccharide

  • Authors:
    • Yinghua Ju
    • Jian Hua
    • Koji Sakamoto
    • Hideoki Ogawa
    • Isao Nagaoka
  • View Affiliations

  • Published online on: December 1, 2008     https://doi.org/10.3892/ijmm_00000089
  • Pages: 809-815
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Abstract

Atherosclerosis is now considered a chronic inflammatory disease, and glucosamine has the potential to exhibit an anti-inflammatory action. Thus, we investigated the effect of glucosamine on tumor necrosis factor α (TNF-α)-induced endothelial cell activation. Human umbilical vein endothelial cells (HUVECs) were stimulated by TNF-α in the presence or absence of glucosamine or its analogue, N-acetylglucosamine. mRNA expression of MCP-1 (a chemoattractant protein) and ICAM-1 (an adhesion molecule) was evaluated by real-time RT-PCR, and their protein levels were analyzed by ELISA and Western blotting, respectively. Furthermore, the effects of glucosamine on the phosphorylation of p38MAPK and NF-κB, and O-N-acetylglucosamine (O-GlcNAc) modification were evaluated by Western blotting. The results demonstrated that glucosamine but not N-acetylglucosamine suppressed TNF-α-induced expression of MCP-1 and ICAM-1 at both the mRNA and protein levels. Furthermore, glucosamine abrogated the phosphorylation of p38MAPK and NF-κB. To note, glucosamine induced O-GlcNAc modification, which was negatively correlated with the expression of MCP-1 and ICAM-1, and phosphorylation of p38MAPK and NF-κB. Thus, glucosamine is likely to suppress endothelial cell activation (TNF-α-induced ICAM-1 and MCP-1 expression) possibly by affecting p38MAPK and NF-κB signaling via O-GlcNAc modification.

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December 2008
Volume 22 Issue 6

Print ISSN: 1107-3756
Online ISSN:1791-244X

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Spandidos Publications style
Ju Y, Hua J, Sakamoto K, Ogawa H and Nagaoka I: Modulation of TNF-α-induced endothelial cell activation by glucosamine, a naturally occurring amino monosaccharide. Int J Mol Med 22: 809-815, 2008.
APA
Ju, Y., Hua, J., Sakamoto, K., Ogawa, H., & Nagaoka, I. (2008). Modulation of TNF-α-induced endothelial cell activation by glucosamine, a naturally occurring amino monosaccharide. International Journal of Molecular Medicine, 22, 809-815. https://doi.org/10.3892/ijmm_00000089
MLA
Ju, Y., Hua, J., Sakamoto, K., Ogawa, H., Nagaoka, I."Modulation of TNF-α-induced endothelial cell activation by glucosamine, a naturally occurring amino monosaccharide". International Journal of Molecular Medicine 22.6 (2008): 809-815.
Chicago
Ju, Y., Hua, J., Sakamoto, K., Ogawa, H., Nagaoka, I."Modulation of TNF-α-induced endothelial cell activation by glucosamine, a naturally occurring amino monosaccharide". International Journal of Molecular Medicine 22, no. 6 (2008): 809-815. https://doi.org/10.3892/ijmm_00000089