Alterations in CD44 isoforms and HAS expression in human articular chondrocytes during the de- and re-differentiation processes

Albrecht,Christian; Schlegel,Werner; Eckl,Peter; Jagersberger,Tamara; Sadeghi,Kambis; Berger,Angelika; Vécsei,Vilmos; Marlovits,Stefan

doi:10.3892/ijmm_00000124

February 2009 Volume 23 Issue 2

Full Size Image

Journals

International Journal of Molecular Medicine

International Journal of Molecular Medicine is an international journal devoted to molecular mechanisms of human disease.

International Journal of Oncology

International Journal of Oncology is an international journal devoted to oncology research and cancer treatment.

Molecular Medicine Reports

Covers molecular medicine topics such as pharmacology, pathology, genetics, neuroscience, infectious diseases, molecular cardiology, and molecular surgery.

Oncology Reports

Oncology Reports is an international journal devoted to fundamental and applied research in Oncology.

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine is an international journal devoted to laboratory and clinical medicine.

Oncology Letters

Oncology Letters is an international journal devoted to Experimental and Clinical Oncology.

Biomedical Reports

Explores a wide range of biological and medical fields, including pharmacology, genetics, microbiology, neuroscience, and molecular cardiology.

Molecular and Clinical Oncology

International journal addressing all aspects of oncology research, from tumorigenesis and oncogenes to chemotherapy and metastasis.

World Academy of Sciences Journal

Multidisciplinary open-access journal spanning biochemistry, genetics, neuroscience, environmental health, and synthetic biology.

International Journal of Functional Nutrition

Open-access journal combining biochemistry, pharmacology, immunology, and genetics to advance health through functional nutrition.

International Journal of Epigenetics

Publishes open-access research on using epigenetics to advance understanding and treatment of human disease.

Medicine International

An International Open Access Journal Devoted to General Medicine.

February 2009 Volume 23 Issue 2

Full Size Image

Article

Alterations in CD44 isoforms and HAS expression in human articular chondrocytes during the de- and re-differentiation processes

Authors:
- Christian Albrecht
- Werner Schlegel
- Peter Eckl
- Tamara Jagersberger
- Kambis Sadeghi
- Angelika Berger
- Vilmos Vécsei
- Stefan Marlovits
View Affiliations / Copyright

Affiliations: Medical University of Vienna, Department of Traumatology, Center for Joint and Cartilage, A-1090 Vienna, Austria. christian.albrecht@meduniwien.ac.at
Pages: 253-259
|
Published online on: February 1, 2009

https://doi.org/10.3892/ijmm_00000124
Expand metrics +

Abstract

The purpose of this study was to investigate the expression of different CD44 and hyaluronan synthase isoforms in cartilage, their alterations during the chondrocyte dedifferentiation process in monolayer culture and during the redifferentiation process on 3D scaffolds. Chondrocytes isolated from human articular cartilage were cultured as a monolayer for up to 36 days and were seeded on two different 3D scaffolds (HYAFF® 11 and Bio-Gide®). Expression levels of CD44s, CD44-lt, CD44-st, HAS1, HAS2, HAS3 and UDPGD were determined by real-time RT-PCR at different time points. At the protein level CD44 and CD90 were analyzed by flow cytometry. HAS2 was found to be the predominantly expressed hyaluronan synthase in chondrocytes and was not subjected to any regulation during the dedifferentiation process. CD44s, CD44-lt, CD44-st and UDPGD, however, were upregulated immediately after cell isolation. In addition, a high cell density was found to significantly increase CD44-st and CD44-lt expression. Redifferentiation on 3D scaffolds reversed the increase of the CD44 expression. Our data point out that CD44 expression does not correlate with matrix assembly in chondrocytes and that CD44 has a regulatory function in chondrocytes, not necessarily on differentiation, but probably on proliferation.

Journals

International Journal of Molecular Medicine

International Journal of Oncology

Molecular Medicine Reports

Oncology Reports

Experimental and Therapeutic Medicine

Oncology Letters

Biomedical Reports

Molecular and Clinical Oncology

World Academy of Sciences Journal

International Journal of Functional Nutrition

International Journal of Epigenetics

Medicine International

Alterations in CD44 isoforms and HAS expression in human articular chondrocytes during the de- and re-differentiation processes

This article is mentioned in:

Abstract

Related Articles