Preferred co-localization of chromosome 8 and 21 in myeloid bone marrow cells detected by three dimensional molecular cytogenetics

  • Authors:
    • Marina Manvelyan
    • Philipp Kempf
    • Anja Weise
    • Kristin Mrasek
    • Anita Heller
    • Amelie Lier
    • Klaus Höffken
    • Hans-Jörg Fricke
    • Herbert G. Sayer
    • Thomas Liehr
    • Hasmik Mkrtchyan
  • View Affiliations

  • Published online on: September 1, 2009     https://doi.org/10.3892/ijmm_00000237
  • Pages: 335-341
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Abstract

The impact of chromosome architecture in the formation of chromosome aberrations is a recent finding of interphase directed molecular cytogenetic studies. Also positive correlation of translocation frequencies and spatial proximity of chromosomes was described. Thus, disease specific chromosomal translocations could be due to tissue specific genomic organization. However, no three-dimensional interphase fluorescence in situ hybridization (FISH) studies for the nuclear architecture of bone marrow (BM) cells have previously been done. In this study, BM of three secondary acute myelogenous leukemia (AML) cases with trisomy 8 and otherwise normal karyotype were evaluated. Bone marrow cells of one AML and one ALL (acute lymphoblastic leukemia) case, peripheral blood lymphocytes and human sperm, all of them with normal karyotype, served as controls. Multicolor banding (MCB) probes for chromosomes 8 and 21 were applied in suspension-FISH (S-FISH). Interestingly, in myeloid bone marrow cells chromosomes 8 (di- and trisomic) and 21 tended to co-localize with their homologue chromosome(s), rather than to be separated. Thus, the co-localization of chromosomes 8 and 21 might promote a translocation providing a selective advantage of t(8;21) cells in AML-M2. In summary, the concept that tissue specific spatial proximity of chromosomes leads to enhanced translocation frequencies was further supported.

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September 2009
Volume 24 Issue 3

Print ISSN: 1107-3756
Online ISSN:1791-244X

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Spandidos Publications style
Manvelyan M, Kempf P, Weise A, Mrasek K, Heller A, Lier A, Höffken K, Fricke H, Sayer HG, Liehr T, Liehr T, et al: Preferred co-localization of chromosome 8 and 21 in myeloid bone marrow cells detected by three dimensional molecular cytogenetics. Int J Mol Med 24: 335-341, 2009.
APA
Manvelyan, M., Kempf, P., Weise, A., Mrasek, K., Heller, A., Lier, A. ... Mkrtchyan, H. (2009). Preferred co-localization of chromosome 8 and 21 in myeloid bone marrow cells detected by three dimensional molecular cytogenetics. International Journal of Molecular Medicine, 24, 335-341. https://doi.org/10.3892/ijmm_00000237
MLA
Manvelyan, M., Kempf, P., Weise, A., Mrasek, K., Heller, A., Lier, A., Höffken, K., Fricke, H., Sayer, H. G., Liehr, T., Mkrtchyan, H."Preferred co-localization of chromosome 8 and 21 in myeloid bone marrow cells detected by three dimensional molecular cytogenetics". International Journal of Molecular Medicine 24.3 (2009): 335-341.
Chicago
Manvelyan, M., Kempf, P., Weise, A., Mrasek, K., Heller, A., Lier, A., Höffken, K., Fricke, H., Sayer, H. G., Liehr, T., Mkrtchyan, H."Preferred co-localization of chromosome 8 and 21 in myeloid bone marrow cells detected by three dimensional molecular cytogenetics". International Journal of Molecular Medicine 24, no. 3 (2009): 335-341. https://doi.org/10.3892/ijmm_00000237