PTEN/pAkt/p53 signaling pathway correlates with the radioresponse of non-small cell lung cancer

  • Authors:
    • Il Lae Jung
    • Hyo Jin Kang
    • Kug Chan Kim
    • In Gyu Kim
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  • Published online on: April 1, 2010     https://doi.org/10.3892/ijmm_00000372
  • Pages: 517-523
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Abstract

The sensitivity or resistance of cancer cells and normal tissues to ionizing radiation plays an important role in the clinical setting of lung cancer treatment. However, to date the exact molecular mechanisms of intrinsic radiosensitivity have not been well explained. In this study, we compared the radiosensitivity or radioresistance in two non-small cell lung cancers (NSCLCs), H460 and A549, and investigated the signaling pathways that confer radioresistance. H460 cells showed a significant G2/M arrest after 12 h of irradiation (5 Gy), reaching 60% of G2/M phase arrest. A549 cells also showed a significant G2/M arrest after 12 h of exposure; however, this arrest completely disappeared after 24 h of exposure. A549 has higher methylated CpG sites in PTEN, which is correlated with tumor radioresistance in some cancer cells, than H460 cells, and the average of the extent of the methylation was ≈4.3 times higher in A549 cells than in H460 cells. As a result, PTEN expression was lower in A549 than in H460. Conducting Western blot analysis, we found that PTEN acted as a negative regulator for pAkt, and the pAkt acted as a negative regulator for p53 expression. According to the above results, we concluded that the radiosensitivity shown in H460 cells may be due to the higher expression of PTEN through p53 signaling pathway.

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April 2010
Volume 25 Issue 4

Print ISSN: 1107-3756
Online ISSN:1791-244X

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Spandidos Publications style
Jung IL, Kang HJ, Kim KC and Kim IG: PTEN/pAkt/p53 signaling pathway correlates with the radioresponse of non-small cell lung cancer. Int J Mol Med 25: 517-523, 2010
APA
Jung, I.L., Kang, H.J., Kim, K.C., & Kim, I.G. (2010). PTEN/pAkt/p53 signaling pathway correlates with the radioresponse of non-small cell lung cancer. International Journal of Molecular Medicine, 25, 517-523. https://doi.org/10.3892/ijmm_00000372
MLA
Jung, I. L., Kang, H. J., Kim, K. C., Kim, I. G."PTEN/pAkt/p53 signaling pathway correlates with the radioresponse of non-small cell lung cancer". International Journal of Molecular Medicine 25.4 (2010): 517-523.
Chicago
Jung, I. L., Kang, H. J., Kim, K. C., Kim, I. G."PTEN/pAkt/p53 signaling pathway correlates with the radioresponse of non-small cell lung cancer". International Journal of Molecular Medicine 25, no. 4 (2010): 517-523. https://doi.org/10.3892/ijmm_00000372