Pinus massoniana bark extract selectively induces apoptosis in human hepatoma cells, possibly through caspase-dependent pathways

  • Authors:
    • Hongling Ma
    • Bing Liu
    • Dongru Feng
    • Heng Xie
    • Ruoda Li
    • Yixing Yuchi
    • Hongbin Wang
    • Jinfa Wang
  • View Affiliations

  • Published online on: May 1, 2010     https://doi.org/10.3892/ijmm_00000401
  • Pages: 751-759
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Abstract

Pinus massoniana bark extract (PMBE) is a mixture of flavonoids, whose antioxidant and apoptosis-inducing properties have been confirmed in vitro. In this study, the apoptotic effect and mechanism of PMBE in HepG2 human hepatoma cells were evaluated. PMBE exerted dose- and time-dependent cell growth inhibition on HepG2 cells, and selectively induced apoptosis without impact on normal liver L-02 cells. Apoptosis induced by PMBE in HepG2 cells was also confirmed by annexin-V/PI staining, transmission electron microscopy and sub-G1 phase accumulation. Moreover, PMBE also slightly blocked the cell cycle in the G2/M and S phases in HepG2 cells. The investigation of the mechanism by which PMBE induced apoptosis in HepG2 cells indicated that activation of extrinsic and intrinsic caspase, inhibition of NF-κB activation and decrease of the antiapoptotic protein Bcl-2 and the intact Bid protein were involved. Furthermore, the antitumor activity of PBME was demonstrated in vivo by a 42.88-69.94% reduction rate of tumor weight in H22 tumor-implanted mice. Taken together, these data indicate that PMBE selectively induces apoptosis in HepG2 cells through caspase-dependent pathways, and inhibits tumor growth in vivo, making it a potential candidate for anticancer therapeutics.

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May 2010
Volume 25 Issue 5

Print ISSN: 1107-3756
Online ISSN:1791-244X

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Spandidos Publications style
Ma H, Liu B, Feng D, Xie H, Li R, Yuchi Y, Wang H and Wang J: Pinus massoniana bark extract selectively induces apoptosis in human hepatoma cells, possibly through caspase-dependent pathways. Int J Mol Med 25: 751-759, 2010
APA
Ma, H., Liu, B., Feng, D., Xie, H., Li, R., Yuchi, Y. ... Wang, J. (2010). Pinus massoniana bark extract selectively induces apoptosis in human hepatoma cells, possibly through caspase-dependent pathways. International Journal of Molecular Medicine, 25, 751-759. https://doi.org/10.3892/ijmm_00000401
MLA
Ma, H., Liu, B., Feng, D., Xie, H., Li, R., Yuchi, Y., Wang, H., Wang, J."Pinus massoniana bark extract selectively induces apoptosis in human hepatoma cells, possibly through caspase-dependent pathways". International Journal of Molecular Medicine 25.5 (2010): 751-759.
Chicago
Ma, H., Liu, B., Feng, D., Xie, H., Li, R., Yuchi, Y., Wang, H., Wang, J."Pinus massoniana bark extract selectively induces apoptosis in human hepatoma cells, possibly through caspase-dependent pathways". International Journal of Molecular Medicine 25, no. 5 (2010): 751-759. https://doi.org/10.3892/ijmm_00000401