Osteoclast activation by receptor activator of NF-κB ligand enhances the mobilization of hematopoietic progenitor cells from the bone marrow in acute injury

  • Authors:
    • Kyung-Ah Cho
    • Sun-Young Joo
    • Ho-Seong Han
    • Kyung-Ha Ryu
    • So-Youn Woo
  • View Affiliations

  • Published online on: October 1, 2010     https://doi.org/10.3892/ijmm_00000499
  • Pages: 557-563
Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )
Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )


Abstract

Osteoclasts (OCLs) are multinucleated cells that are derived from the monocyte/macrophage hematopoietic lineage in response to receptor activator of NF-κB ligand (RANKL) activation. They are specialized cells responsible for physiological bone resorption and as well as pathologic bone loss. In addition to their unique ability to resorb bone, OCLs also play a potential role in the mobilization of hematopoietic progenitor cells from the bone marrow (BM), particularly under various stress stimuli (e.g. hypoxia, injury or inflammation). We investigated the effect of activated OCLs on the stem cell niche and whether this leads to mobilization of hematopoietic progenitors. We induced activated OCLs from the RAW264.7 cell line through stimulation with RANKL and we quantified the levels of the stem cell niche component SDF-1 on the osteblasts and CXCR4 on the bone marrow cells (BMCs) by culturing with supernatants from activated OCLs. In addition, we exposed mice to stress by inducing liver injury with CCl4 followed by injecting RANKL to activate OCLs and compared the effect on the mobilization of hematopoietic progenitor cells from the BM. We found that functional OCLs cleaved SDF-1α in the osteoblasts and increased CXCR4 expression in the BMCs. Moreover, under stress in vivo, mobilized hematopoietic progenitor cells were significantly increased after RANKL treatment. These results suggest that OCLs might be involved in alteration of the interaction between SDF-1 and CXCR4 leading to mobilization of hematopoietic progenitor cells from the BM.

Related Articles

Journal Cover

October 2010
Volume 26 Issue 4

Print ISSN: 1107-3756
Online ISSN:1791-244X

Sign up for eToc alerts

Recommend to Library

Copy and paste a formatted citation
x
Spandidos Publications style
Cho K, Joo S, Han H, Ryu K and Woo S: Osteoclast activation by receptor activator of NF-κB ligand enhances the mobilization of hematopoietic progenitor cells from the bone marrow in acute injury . Int J Mol Med 26: 557-563, 2010.
APA
Cho, K., Joo, S., Han, H., Ryu, K., & Woo, S. (2010). Osteoclast activation by receptor activator of NF-κB ligand enhances the mobilization of hematopoietic progenitor cells from the bone marrow in acute injury . International Journal of Molecular Medicine, 26, 557-563. https://doi.org/10.3892/ijmm_00000499
MLA
Cho, K., Joo, S., Han, H., Ryu, K., Woo, S."Osteoclast activation by receptor activator of NF-κB ligand enhances the mobilization of hematopoietic progenitor cells from the bone marrow in acute injury ". International Journal of Molecular Medicine 26.4 (2010): 557-563.
Chicago
Cho, K., Joo, S., Han, H., Ryu, K., Woo, S."Osteoclast activation by receptor activator of NF-κB ligand enhances the mobilization of hematopoietic progenitor cells from the bone marrow in acute injury ". International Journal of Molecular Medicine 26, no. 4 (2010): 557-563. https://doi.org/10.3892/ijmm_00000499