Proteomic analysis of oxidative stress-induced neuronal cell death by using two-dimensional fluorescence difference gel electrophoresis

  • Authors:
    • Eun Young Kim
    • Tae-Sung Yoon
    • Young Jae Bahn
    • Dae Gwin Jeong
    • Mi Rim Park
    • Sang J. Chung
    • Sung Goo Park
    • Byoung Chul Park
    • Sang Chul Lee
    • Seong Eon Ryu
    • Kwang-Hee Bae
  • View Affiliations

  • Published online on: December 1, 2010     https://doi.org/10.3892/ijmm_00000531
  • Pages: 829-835
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Abstract

Oxidative stress has been implicated in a number of neurological disorders, including cerebral ischemia and neuro-degenerative diseases. Comprehensive proteomic studies were carried out using an immortalized mouse hippocampal cell line, HT22, exhibiting oxidative stress-mediated cell death upon glutamate treatment. Two-dimensional fluorescence difference gel electrophoresis (2D DIGE) of subcellular organelle fractions revealed that significant numbers of proteins showed quantitative changes during HT22 cell death, among which a total of 51 proteins were identified by mass spectrometry. The identified proteins indicate that HT22 cell death occurs through perturbations in mitochondrial function, changes in translational elongation machinery, and translocation of proteins across subcellular organelles. This list of proteins may shed light on oxidative stress-mediated neuronal cell death.

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December 2010
Volume 26 Issue 6

Print ISSN: 1107-3756
Online ISSN:1791-244X

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Spandidos Publications style
Kim EY, Yoon T, Bahn YJ, Jeong DG, Park MR, Chung SJ, Park SG, Park BC, Lee SC, Ryu SE, Ryu SE, et al: Proteomic analysis of oxidative stress-induced neuronal cell death by using two-dimensional fluorescence difference gel electrophoresis . Int J Mol Med 26: 829-835, 2010
APA
Kim, E.Y., Yoon, T., Bahn, Y.J., Jeong, D.G., Park, M.R., Chung, S.J. ... Bae, K. (2010). Proteomic analysis of oxidative stress-induced neuronal cell death by using two-dimensional fluorescence difference gel electrophoresis . International Journal of Molecular Medicine, 26, 829-835. https://doi.org/10.3892/ijmm_00000531
MLA
Kim, E. Y., Yoon, T., Bahn, Y. J., Jeong, D. G., Park, M. R., Chung, S. J., Park, S. G., Park, B. C., Lee, S. C., Ryu, S. E., Bae, K."Proteomic analysis of oxidative stress-induced neuronal cell death by using two-dimensional fluorescence difference gel electrophoresis ". International Journal of Molecular Medicine 26.6 (2010): 829-835.
Chicago
Kim, E. Y., Yoon, T., Bahn, Y. J., Jeong, D. G., Park, M. R., Chung, S. J., Park, S. G., Park, B. C., Lee, S. C., Ryu, S. E., Bae, K."Proteomic analysis of oxidative stress-induced neuronal cell death by using two-dimensional fluorescence difference gel electrophoresis ". International Journal of Molecular Medicine 26, no. 6 (2010): 829-835. https://doi.org/10.3892/ijmm_00000531