T lymphocyte responses against hepatitis B virus-related hepatocellular carcinoma induced by adenovirus vaccine encoding HBx

  • Authors:
    • Yuhua Li
    • Ping Cheng
    • Yanjun Wen
    • Ping Chen
    • Li Yang
    • Xia Zhao
    • Huimin Lv
    • Qingli Quan
    • Yonglin Wu
    • Huiqiang Yang
    • Jie Liu
    • Xueru Wen
    • Nina Liu
    • Zhuang Kang
    • Shan Luo
    • Lian Wang
    • Yuquan Wei
  • View Affiliations

  • Published online on: December 1, 2010     https://doi.org/10.3892/ijmm_00000536
  • Pages: 869-876
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Abstract

HBx is an oncogenic tumor-associated antigen and is dominantly expressed in hepatitis and hepatoma tissues, the induction of active cellular responses against HBx should be a promising approach for the treatment of hepatitis B virus-related hepatocellular carcinoma. The present study was designed to test whether a replication-defective adenovirus vaccine expressing HBx antigen could be effectively used in the immunotherapy of hepatocellular carcinoma. To validate the possibility, we developed a novel HBx-positive hepatocellular carcinoma in mice by inoculated the pcDNA-HBx transfected Hepa1-6 cells subcutaneously into the right flank of mice. We found that immunotherapy with Ad-HBx was effective at both protective and therapeutic antitumor immunity in the hepatoma models in immune-competent mice. Histological examination revealed that Ad-HBx treatment led to significantly increased induction of apoptosis, tumor necrosis, and elevated CD8+ lymphocyte infiltration. In addition, the induction efficacy of the CTL response is dramatically enhanced by immunotherapy. Cytokine analysis comfirmed that the antitumor efficacy of Ad-HBx may mostly result from cellular immunity. Our findings may prove useful in development of adenovirus vaccine based on HBx antigen to the treatment of HBV-associated hepatocellular carcinoma.

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December 2010
Volume 26 Issue 6

Print ISSN: 1107-3756
Online ISSN:1791-244X

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Spandidos Publications style
Li Y, Cheng P, Wen Y, Chen P, Yang L, Zhao X, Lv H, Quan Q, Wu Y, Yang H, Yang H, et al: T lymphocyte responses against hepatitis B virus-related hepatocellular carcinoma induced by adenovirus vaccine encoding HBx . Int J Mol Med 26: 869-876, 2010
APA
Li, Y., Cheng, P., Wen, Y., Chen, P., Yang, L., Zhao, X. ... Wei, Y. (2010). T lymphocyte responses against hepatitis B virus-related hepatocellular carcinoma induced by adenovirus vaccine encoding HBx . International Journal of Molecular Medicine, 26, 869-876. https://doi.org/10.3892/ijmm_00000536
MLA
Li, Y., Cheng, P., Wen, Y., Chen, P., Yang, L., Zhao, X., Lv, H., Quan, Q., Wu, Y., Yang, H., Liu, J., Wen, X., Liu, N., Kang, Z., Luo, S., Wang, L., Wei, Y." T lymphocyte responses against hepatitis B virus-related hepatocellular carcinoma induced by adenovirus vaccine encoding HBx ". International Journal of Molecular Medicine 26.6 (2010): 869-876.
Chicago
Li, Y., Cheng, P., Wen, Y., Chen, P., Yang, L., Zhao, X., Lv, H., Quan, Q., Wu, Y., Yang, H., Liu, J., Wen, X., Liu, N., Kang, Z., Luo, S., Wang, L., Wei, Y." T lymphocyte responses against hepatitis B virus-related hepatocellular carcinoma induced by adenovirus vaccine encoding HBx ". International Journal of Molecular Medicine 26, no. 6 (2010): 869-876. https://doi.org/10.3892/ijmm_00000536