The PTEN tumor suppressor inhibits human airway smooth muscle cell migration

  • Authors:
    • Haibing Lan
    • Haohai Zhong
    • Yang Gao
    • Dunqiang Ren
    • Lili Chen
    • Dacheng Zhang
    • Wenyan Lai
    • Jian Xu
    • Yaling Luo
  • View Affiliations

  • Published online on: December 1, 2010     https://doi.org/10.3892/ijmm_00000539
  • Pages: 893-899
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Abstract

Airway remodeling in asthma is characterized by increased airway smooth muscle (ASM) mass, accompanied by cell migration. It is well known that the proliferation and migration of ASM cells (ASMCs) play a key role in airway remodeling, but the precise mechanism modulating these cellular events remains unclear. One of the genes most likely to be involved in this process is the phosphatase and tensin homolog (PTEN) gene, whose deletion from chromosome 10 can inhibit the proliferation and migration of many cell types. In this study, we investigated the effects of PTEN on human ASMCs. The cells were infected with recombinant adenovirus containing wild-type PTEN cDNA (Ad-PTEN), and the results were compared with those from the uninfected cells and those infected with the GFP-labeled adenovirus vector. Cell proliferation was measured using the MTT method. Cell migration was determined by wound-healing and transwell assays. The expressions of PTEN, phospho-Akt, Akt, phospho-ERK1/2, ERK1/2, phospho-focal adhesion kinase (FAK) and FAK, were examined by Western blot analysis. The results show that PTEN is expressed endogenously in ASMCs, and that Ad-PTEN inhibits the proliferation and migration of these cells. In addition, the Ad-PTEN treatment decreased the phosphorylation of Akt and FAK but not that of ERK1/2. In conclusion, this study demonstrates that PTEN overexpression inhibits the proliferation and migration of human ASMCs by down-regulating the activity of the Akt and FAK signaling pathways.

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December 2010
Volume 26 Issue 6

Print ISSN: 1107-3756
Online ISSN:1791-244X

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Spandidos Publications style
Lan H, Zhong H, Gao Y, Ren D, Chen L, Zhang D, Lai W, Xu J and Luo Y: The PTEN tumor suppressor inhibits human airway smooth muscle cell migration . Int J Mol Med 26: 893-899, 2010.
APA
Lan, H., Zhong, H., Gao, Y., Ren, D., Chen, L., Zhang, D. ... Luo, Y. (2010). The PTEN tumor suppressor inhibits human airway smooth muscle cell migration . International Journal of Molecular Medicine, 26, 893-899. https://doi.org/10.3892/ijmm_00000539
MLA
Lan, H., Zhong, H., Gao, Y., Ren, D., Chen, L., Zhang, D., Lai, W., Xu, J., Luo, Y."The PTEN tumor suppressor inhibits human airway smooth muscle cell migration ". International Journal of Molecular Medicine 26.6 (2010): 893-899.
Chicago
Lan, H., Zhong, H., Gao, Y., Ren, D., Chen, L., Zhang, D., Lai, W., Xu, J., Luo, Y."The PTEN tumor suppressor inhibits human airway smooth muscle cell migration ". International Journal of Molecular Medicine 26, no. 6 (2010): 893-899. https://doi.org/10.3892/ijmm_00000539