Comparison of different classes of CpG-ODN in augmenting the generation of human epitope peptide-specific CTLs

Katsuda,Masahiro; Iwahashi,Makoto; Matsuda,Kenji; Miyazawa,Motoki; Nakamori,Mikihito; Nakamura,Masaki; Ojima,Toshiyasu; Iida,Takeshi; Hayata,Keiji; Yamaue,Hiroki

doi:10.3892/ijo.2011.1146

November 2011 Volume 39 Issue 5

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November 2011 Volume 39 Issue 5

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Article

Comparison of different classes of CpG-ODN in augmenting the generation of human epitope peptide-specific CTLs

Authors:
- Masahiro Katsuda
- Makoto Iwahashi
- Kenji Matsuda
- Motoki Miyazawa
- Mikihito Nakamori
- Masaki Nakamura
- Toshiyasu Ojima
- Takeshi Iida
- Keiji Hayata
- Hiroki Yamaue
View Affiliations / Copyright

Affiliations: Second Department of Surgery, School of Medicine, Wakayama Medical University, Wakayama, Japan, Second Department of Surgery, School of Medicine, Wakayama Medical University, 811-1 Kimiidera, Wakayama 641-8510, Japan
Pages: 1295-1302
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Published online on: July 28, 2011

https://doi.org/10.3892/ijo.2011.1146
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Abstract

Three distinct classes of CpG-oligonucleotides (ODN) (CpG-A, CpG-B and CpG-C) have been identified on the basis of differences in their structures and immune effects. To date, only CpG-B is applied for clinical treatments; however, it is still unknown which of the different CpG-ODN classes is most useful as an adjuvant for human cancer vaccine therapy. In the present study, we examined the activity of these 3 types of CpG-ODN in enhancing the induction of human peptide-specific CTLs. Our data showed that the specific cytotoxicity was augmented in the presence of CpG-A, -B and -C but not in the presence of control ODN, and the augmenting effect was most potent with CpG-A. Flow cytometric analysis showed the subpopulation of effector-memory cells in CD8+ cells was most increased with CpG-A. Furthermore, depletion of PDCs from PBMCs before stimulation with peptide and CpG-ODN completely abrogated the augmenting effect of CpG-ODN. These data indicated that the stimulation of PDCs by CpG-ODN augmented the generation of peptide-specific CTLs, and CpG-A was superior to CpG-B and CpG-C in terms of augmenting the generation of human peptide-specific CTLs in vitro.

Journals

International Journal of Molecular Medicine

International Journal of Oncology

Molecular Medicine Reports

Oncology Reports

Experimental and Therapeutic Medicine

Oncology Letters

Biomedical Reports

Molecular and Clinical Oncology

World Academy of Sciences Journal

International Journal of Functional Nutrition

International Journal of Epigenetics

Medicine International

Comparison of different classes of CpG-ODN in augmenting the generation of human epitope peptide-specific CTLs

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Abstract

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