Comparison of different classes of CpG-ODN in augmenting the generation of human epitope peptide-specific CTLs

  • Authors:
    • Masahiro Katsuda
    • Makoto Iwahashi
    • Kenji Matsuda
    • Motoki Miyazawa
    • Mikihito Nakamori
    • Masaki Nakamura
    • Toshiyasu Ojima
    • Takeshi Iida
    • Keiji Hayata
    • Hiroki Yamaue
  • View Affiliations

  • Published online on: July 28, 2011     https://doi.org/10.3892/ijo.2011.1146
  • Pages: 1295-1302
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Abstract

Three distinct classes of CpG-oligonucleotides (ODN) (CpG-A, CpG-B and CpG-C) have been identified on the basis of differences in their structures and immune effects. To date, only CpG-B is applied for clinical treatments; however, it is still unknown which of the different CpG-ODN classes is most useful as an adjuvant for human cancer vaccine therapy. In the present study, we examined the activity of these 3 types of CpG-ODN in enhancing the induction of human peptide-specific CTLs. Our data showed that the specific cytotoxicity was augmented in the presence of CpG-A, -B and -C but not in the presence of control ODN, and the augmenting effect was most potent with CpG-A. Flow cytometric analysis showed the subpopulation of effector-memory cells in CD8+ cells was most increased with CpG-A. Furthermore, depletion of PDCs from PBMCs before stimulation with peptide and CpG-ODN completely abrogated the augmenting effect of CpG-ODN. These data indicated that the stimulation of PDCs by CpG-ODN augmented the generation of peptide-specific CTLs, and CpG-A was superior to CpG-B and CpG-C in terms of augmenting the generation of human peptide-specific CTLs in vitro.

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November 2011
Volume 39 Issue 5

Print ISSN: 1019-6439
Online ISSN:1791-2423

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APA
Katsuda, M., Iwahashi, M., Matsuda, K., Miyazawa, M., Nakamori, M., Nakamura, M. ... Yamaue, H. (2011). Comparison of different classes of CpG-ODN in augmenting the generation of human epitope peptide-specific CTLs. International Journal of Oncology, 39, 1295-1302. https://doi.org/10.3892/ijo.2011.1146
MLA
Katsuda, M., Iwahashi, M., Matsuda, K., Miyazawa, M., Nakamori, M., Nakamura, M., Ojima, T., Iida, T., Hayata, K., Yamaue, H."Comparison of different classes of CpG-ODN in augmenting the generation of human epitope peptide-specific CTLs". International Journal of Oncology 39.5 (2011): 1295-1302.
Chicago
Katsuda, M., Iwahashi, M., Matsuda, K., Miyazawa, M., Nakamori, M., Nakamura, M., Ojima, T., Iida, T., Hayata, K., Yamaue, H."Comparison of different classes of CpG-ODN in augmenting the generation of human epitope peptide-specific CTLs". International Journal of Oncology 39, no. 5 (2011): 1295-1302. https://doi.org/10.3892/ijo.2011.1146