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Article

In vitro expansion of human glioblastoma cells at non-physiological oxygen tension irreversibly alters subsequent in vivo aggressiveness and AC133 expression

  • Authors:
    • Erika Bourseau-Guilmain
    • Laurent Lemaire
    • Audrey Griveau
    • Eric Hervouet
    • François Vallette
    • François Berger
    • Philippe Menei
    • Jean-Pierre Benoit
    • Didier Wion
    • Emmanuel Garcion
  • View Affiliations / Copyright

    Affiliations: Laboratoire d'Ingénierie de la Vectorisation Particulaire, Inserm, UMR_S 646, Université d'Angers, Angers, France, Institut National de la Santé et de la Recherche Médicale, Inserm U646, IBS - CHU Angers, 4 rue Larrey, 49933 Angers cedex 9, France
  • Pages: 1220-1229
    |
    Published online on: November 23, 2011
       https://doi.org/10.3892/ijo.2011.1271
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Abstract

Among markers of glioblastoma initiating cells, AC133 has been shown to be associated with glioblastoma resistance and malignancy. Recently, it was demonstrated that increasing oxygen tension (pO2) down-regulated AC133 expression in glioblastoma cells in vitro. In order to better understand extrinsic factor regulation of AC133, this work aimed to investigate the relationship between cell culture pO2, AC133 expression, and tumor development and phenotype. Using treatments with CoCl2 and HIF-1α shRNA knockdowns on non-sorted human primary glioblastoma cells cultured at low (3%) versus high (21%) oxygen tension, we established a responsibility for low pO2 in the maintenance of high levels of AC133 expression, with a major but non-exclusive role for HIF-1α. We also demonstrated that human glioblastoma cells previously cultured under high oxygen tension can lose part of their aggressiveness when orthotopically engrafted in SCID mice or lead to tumors with distinct phenotypes and no re-expression of AC133. These observations showed that the specific pO2 microenvironment irreversibly impacts glioblastoma cell phenotypes, highlighting the pertinence of culture conditions when extrapolating data from xenogenic models to human cells in their source environment. They also raised AC133 as a marker of non-exposure to oxygenated areas rather than a marker of aggressiveness or low pO2 niches.
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1 

Ignatova TN, Kukekov VG, Laywell ED, Suslov ON, Vrionis FD and Steindler DA: Human cortical glial tumors contain neural stem-like cells expressing astroglial and neuronal markers in vitro. Glia. 39:193–206. 2002.

2 

Singh SK, Clarke ID, Terasaki M, et al: Identification of a cancer stem cell in human brain tumors. Cancer Res. 63:5821–5828. 2003.

3 

Singh SK, Hawkins C, Clarke ID, et al: Identification of human brain tumour initiating cells. Nature. 432:396–401. 2004.

4 

Jacques TS, Swales A, Brzozowski MJ, et al: Combinations of genetic mutations in the adult neural stem cell compartment determine brain tumour phenotypes. EMBO J. 29:222–235. 2010.

5 

Visvader JE and Lindeman GJ: Cancer stem cells in solid tumours: accumulating evidence and unresolved questions. Nat Rev Cancer. 8:755–768. 2008.

6 

Mayol JF, Loeuillet C, Herodin F and Wion D: Characterisation of normal and cancer stem cells: one experimental paradigm for two kinds of stem cells. Bioessays. 31:993–1001. 2009.

7 

Mao XG, Zhang X, Xue XY, et al: Brain tumor stem-like cells identified by neural stem cell marker CD15. Transl Oncol. 2:247–257. 2009.

8 

Bao S, Wu Q, Li Z, et al: Targeting cancer stem cells through L1CAM suppresses glioma growth. Cancer Res. 68:6043–6048. 2008.

9 

Miraglia S, Godfrey W, Yin AH, et al: A novel five-transmembrane hematopoietic stem cell antigen: isolation, characterization, and molecular cloning. Blood. 90:5013–5021. 1997.

10 

Yin AH, Miraglia S, Zanjani ED, et al: AC133, a novel marker for human hematopoietic stem and progenitor cells. Blood. 90:5002–5012. 1997.

11 

Corbeil D, Roper K, Weigmann A and Huttner WB: AC133 hematopoietic stem cell antigen: human homologue of mouse kidney prominin or distinct member of a novel protein family? Blood. 91:2625–2626. 1998.

12 

Ferrandina G, Petrillo M, Bonanno G and Scambia G: Targeting CD133 antigen in cancer. Expert Opin Ther Targets. 13:823–837. 2009.

13 

Bao S, Wu Q, McLendon RE, et al: Glioma stem cells promote radioresistance by preferential activation of the DNA damage response. Nature. 444:756–760. 2006.

14 

Zobalova R, McDermott L, Stantic M, Prokopova K, Dong LF and Neuzil J: CD133-positive cells are resistant to TRAIL due to up-regulation of FLIP. Biochem Biophys Res Commun. 373:567–571. 2008.

15 

Bao S, Wu Q, Sathornsumetee S, et al: Stem cell-like glioma cells promote tumor angiogenesis through vascular endothelial growth factor. Cancer Res. 66:7843–7848. 2006.

16 

Zeppernick F, Ahmadi R, Campos B, et al: Stem cell marker CD133 affects clinical outcome in glioma patients. Clin Cancer Res. 14:123–129. 2008.

17 

Joo KM, Kim SY, Jin X, et al: Clinical and biological implications of CD133-positive and CD133-negative cells in glioblastomas. Lab Invest. 88:808–815. 2008.

18 

Garcion E, Naveilhan P, Berger F and Wion D: Cancer stem cells: Beyond Koch’s postulates. Cancer Lett. 278:3–8. 2008.

19 

Scadden DT: The stem-cell niche as an entity of action. Nature. 441:1075–1079. 2006.

20 

Calabrese C, Poppleton H, Kocak M, et al: A perivascular niche for brain tumor stem cells. Cancer Cell. 11:69–82. 2007.

21 

Li Z, Bao S, Wu Q, et al: Hypoxia-inducible factors regulate tumorigenic capacity of glioma stem cells. Cancer Cell. 15:501–513. 2009.

22 

Pistollato F, Abbadi S, Rampazzo E, et al: Intratumoral hypoxic gradient drives stem cells distribution and MGMT expression in glioblastoma. Stem Cells. 28:851–862. 2010.

23 

Panchision DM: The role of oxygen in regulating neural stem cells in development and disease. J Cell Physiol. 220:562–568. 2009.

24 

Evans SM, Judy KD, Dunphy I, et al: Hypoxia is important in the biology and aggression of human glial brain tumors. Clin Cancer Res. 10:8177–8184. 2004.

25 

Platet N, Liu SY, Atifi ME, et al: Influence of oxygen tension on CD133 phenotype in human glioma cell cultures. Cancer Lett. 258:286–290. 2007.

26 

McCord AM, Jamal M, Shankavarum UT, Lang FF, Camphausen K and Tofilon PJ: Physiologic oxygen concentration enhances the stem-like properties of CD133+ human glioblastoma cells in vitro. Mol Cancer Res. 7:489–497. 2009.

27 

Vandesompele J, De Preter K, Pattyn F, et al: Accurate normalization of real-time quantitative RT-PCR data by geometric averaging of multiple internal control genes. Genome Biol. 3:Research00342002.

28 

Zhong H, De Marzo AM, Laughner E, et al: Overexpression of hypoxia-inducible factor 1alpha in common human cancers and their metastases. Cancer Res. 59:5830–5835. 1999.

29 

Wenger RH, Stiehl DP and Camenisch G: Integration of oxygen signaling at the consensus HRE. Sci STKE. 2005:re122005.

30 

Tazuke SI, Mazure NM, Sugawara J, et al: Hypoxia stimulates insulin-like growth factor binding protein 1 (IGFBP-1) gene expression in HepG2 cells: a possible model for IGFBP-1 expression in fetal hypoxia. Proc Natl Acad Sci USA. 95:10188–10193. 1998.

31 

Yuan Y, Hilliard G, Ferguson T and Millhorn DE: Cobalt inhibits the interaction between hypoxia-inducible factor-alpha and von Hippel-Lindau protein by direct binding to hypoxia-inducible factor-alpha. J Biol Chem. 278:15911–15916. 2003.

32 

Vordermark D and Brown JM: Evaluation of hypoxia-inducible factor-1alpha (HIF-1alpha) as an intrinsic marker of tumor hypoxia in U87 MG human glioblastoma: in vitro and xenograft studies. Int J Radiat Oncol Biol Phys. 56:1184–1193. 2003.

33 

Csete M: Oxygen in the cultivation of stem cells. Ann NY Acad Sci. 1049:1–8. 2005.

34 

Vaupel P, Kelleher DK and Hockel M: Oxygen status of malignant tumors: pathogenesis of hypoxia and significance for tumor therapy. Semin Oncol. 28:29–35. 2001.

35 

Lee J, Kotliarova S, Kotliarov Y, et al: Tumor stem cells derived from glioblastomas cultured in bFGF and EGF more closely mirror the phenotype and genotype of primary tumors than do serum-cultured cell lines. Cancer Cell. 9:391–403. 2006.

36 

Shervington A and Lu C: Expression of multidrug resistance genes in normal and cancer stem cells. Cancer Invest. 26:535–542. 2008.

37 

Huang X, Le QT and Giaccia AJ: MiR-210-micromanager of the hypoxia pathway. Trends Mol Med. 16:230–237. 2010.

38 

Pistollato F, Chen HL, Rood BR, et al: Hypoxia and HIF1alpha repress the differentiative effects of BMPs in high-grade glioma. Stem Cells. 27:7–17. 2009.

39 

Dringen R, Pfeiffer B and Hamprecht B: Synthesis of the antioxidant glutathione in neurons: supply by astrocytes of CysGly as precursor for neuronal glutathione. J Neurosci. 19:562–569. 1999.

40 

King FW, Ritner C, Liszewski W, et al: Subpopulations of human embryonic stem cells with distinct tissue-specific fates can be selected from pluripotent cultures. Stem Cells Dev. 18:1441–1450. 2009.

41 

Wu Y and Wu PY: CD133 as a marker for cancer stem cells: progresses and concerns. Stem Cells Dev. 18:1127–1134. 2009.

42 

Alcantara Llaguno S, Chen J, Kwon CH, et al: Malignant astrocytomas originate from neural stem/progenitor cells in a somatic tumor suppressor mouse model. Cancer Cell. 15:45–56. 2009.

43 

Wang Y, Yang J, Zheng H, et al: Expression of mutant p53 proteins implicates a lineage relationship between neural stem cells and malignant astrocytic glioma in a murine model. Cancer Cell. 15:514–526. 2009.

44 

Hill RP: Identifying cancer stem cells in solid tumors: case not proven. Cancer Res. 66:1890–1895. 2006.

45 

Adams JM and Strasser A: Is tumor growth sustained by rare cancer stem cells or dominant clones? Cancer Res. 68:4018–4021. 2008.

46 

Piccirillo SG, Reynolds BA, Zanetti N, et al: Bone morphogenetic proteins inhibit the tumorigenic potential of human brain tumour-initiating cells. Nature. 444:761–765. 2006.

47 

Ogden AT, Waziri AE, Lochhead RA, et al: Identification of A2B5+CD133− tumor-initiating cells in adult human gliomas. Neurosurgery. 62:505–514. 2008.

48 

Wang J, Sakariassen PO, Tsinkalovsky O, et al: CD133 negative glioma cells form tumors in nude rats and give rise to CD133 positive cells. Int J Cancer. 122:761–768. 2008.

49 

Soeda A, Park M, Lee D, et al: Hypoxia promotes expansion of the CD133-positive glioma stem cells through activation of HIF-1alpha. Oncogene. 28:3949–3959. 2009.

50 

Bar EE, Lin A, Mahairaki V, Matsui W and Eberhart CG: Hypoxia increases the expression of stem-cell markers and promotes clonogenicity in glioblastoma neurospheres. Am J Pathol. 177:1491–1502. 2010.

51 

Matsumoto K, Arao T, Tanaka K, et al: mTOR signal and hypoxia-inducible factor-1 alpha regulate CD133 expression in cancer cells. Cancer Res. 69:7160–7164. 2009.

52 

Heddleston JM, Li Z, McLendon RE, Hjelmeland AB and Rich JN: The hypoxic microenvironment maintains glioblastoma stem cells and promotes reprogramming towards a cancer stem cell phenotype. Cell Cycle. 8:3274–3284. 2009.

53 

Lopez-Barneo J, Pardal R and Ortega-Saenz P: Cellular mechanism of oxygen sensing. Annu Rev Physiol. 63:259–287. 2001.

54 

Arany Z, Foo SY, Ma Y, et al: HIF-independent regulation of VEGF and angiogenesis by the transcriptional coactivator PGC-1alpha. Nature. 451:1008–1012. 2008.

55 

Liu L, Cash TP, Jones RG, Keith B, Thompson CB and Simon MC: Hypoxia-induced energy stress regulates mRNA translation and cell growth. Mol Cell. 21:521–531. 2006.

56 

Tabu K, Sasai K, Kimura T, et al: Promoter hypomethylation regulates CD133 expression in human gliomas. Cell Res. 18:1037–1046. 2008.

57 

Yi JM, Tsai HC, Glockner SC, et al: Abnormal DNA methylation of CD133 in colorectal and glioblastoma tumors. Cancer Res. 68:8094–8103. 2008.

58 

Wion D, Christen T, Barbier EL and Coles JA: PO(2) matters in stem cell culture. Cell Stem Cell. 5:242–243. 2009.

59 

Lillien L and Raphael H: BMP and FGF regulate the development of EGF-responsive neural progenitor cells. Development. 127:4993–5005. 2000.

60 

Chen R, Nishimura MC, Bumbaca SM, et al: A hierarchy of self-renewing tumor-initiating cell types in glioblastoma. Cancer Cell. 17:362–375. 2010.

61 

Kemper K, Sprick MR, De Bree M, et al: The AC133 epitope, but not the CD133 protein, is lost upon cancer stem cell differentiation. Cancer Res. 70:719–729. 2010.

62 

Campos B, Wan F, Farhadi M, et al: Differentiation therapy exerts antitumor effects on stem-like glioma cells. Clin Cancer Res. 16:2715–2728. 2010.

63 

Soda Y, Marumoto T, Friedmann-Morvinski D, et al: Trans-differentiation of glioblastoma cells into vascular endothelial cells. Proc Natl Acad Sci USA. 108:4274–4280. 2011.

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Copy and paste a formatted citation
Spandidos Publications style
Bourseau-Guilmain E, Lemaire L, Griveau A, Hervouet E, Vallette F, Berger F, Menei P, Benoit J, Wion D, Garcion E, Garcion E, et al: In vitro expansion of human glioblastoma cells at non-physiological oxygen tension irreversibly alters subsequent in vivo aggressiveness and AC133 expression. Int J Oncol 40: 1220-1229, 2012.
APA
Bourseau-Guilmain, E., Lemaire, L., Griveau, A., Hervouet, E., Vallette, F., Berger, F. ... Garcion, E. (2012). In vitro expansion of human glioblastoma cells at non-physiological oxygen tension irreversibly alters subsequent in vivo aggressiveness and AC133 expression. International Journal of Oncology, 40, 1220-1229. https://doi.org/10.3892/ijo.2011.1271
MLA
Bourseau-Guilmain, E., Lemaire, L., Griveau, A., Hervouet, E., Vallette, F., Berger, F., Menei, P., Benoit, J., Wion, D., Garcion, E."In vitro expansion of human glioblastoma cells at non-physiological oxygen tension irreversibly alters subsequent in vivo aggressiveness and AC133 expression". International Journal of Oncology 40.4 (2012): 1220-1229.
Chicago
Bourseau-Guilmain, E., Lemaire, L., Griveau, A., Hervouet, E., Vallette, F., Berger, F., Menei, P., Benoit, J., Wion, D., Garcion, E."In vitro expansion of human glioblastoma cells at non-physiological oxygen tension irreversibly alters subsequent in vivo aggressiveness and AC133 expression". International Journal of Oncology 40, no. 4 (2012): 1220-1229. https://doi.org/10.3892/ijo.2011.1271
Copy and paste a formatted citation
x
Spandidos Publications style
Bourseau-Guilmain E, Lemaire L, Griveau A, Hervouet E, Vallette F, Berger F, Menei P, Benoit J, Wion D, Garcion E, Garcion E, et al: In vitro expansion of human glioblastoma cells at non-physiological oxygen tension irreversibly alters subsequent in vivo aggressiveness and AC133 expression. Int J Oncol 40: 1220-1229, 2012.
APA
Bourseau-Guilmain, E., Lemaire, L., Griveau, A., Hervouet, E., Vallette, F., Berger, F. ... Garcion, E. (2012). In vitro expansion of human glioblastoma cells at non-physiological oxygen tension irreversibly alters subsequent in vivo aggressiveness and AC133 expression. International Journal of Oncology, 40, 1220-1229. https://doi.org/10.3892/ijo.2011.1271
MLA
Bourseau-Guilmain, E., Lemaire, L., Griveau, A., Hervouet, E., Vallette, F., Berger, F., Menei, P., Benoit, J., Wion, D., Garcion, E."In vitro expansion of human glioblastoma cells at non-physiological oxygen tension irreversibly alters subsequent in vivo aggressiveness and AC133 expression". International Journal of Oncology 40.4 (2012): 1220-1229.
Chicago
Bourseau-Guilmain, E., Lemaire, L., Griveau, A., Hervouet, E., Vallette, F., Berger, F., Menei, P., Benoit, J., Wion, D., Garcion, E."In vitro expansion of human glioblastoma cells at non-physiological oxygen tension irreversibly alters subsequent in vivo aggressiveness and AC133 expression". International Journal of Oncology 40, no. 4 (2012): 1220-1229. https://doi.org/10.3892/ijo.2011.1271
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