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International Journal of Oncology
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Print ISSN: 1019-6439 Online ISSN: 1791-2423
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Article

RNAi-mediated knockdown of ERK1/2 inhibits cell proliferation and invasion and increases chemosensitivity to cisplatin in human osteosarcoma U2-OS cells in vitro

  • Authors:
    • Haipeng Si
    • Changliang Peng
    • Jingjing Li
    • Xiqian Wang
    • Lianwen Zhai
    • Xiaofeng Li
    • Jianmin Li
  • View Affiliations / Copyright

    Affiliations: Department of Orthopaedics, Second Affiliated Hospital, Shandong University, 247 Beiyuan Da Street, Jinan 250033, P.R. China, Department of Orthopaedics, Qilu Hospital, Shandong University, 107 Wen Hua Xi Road, Jinan 250012, P.R. China
  • Pages: 1291-1297
    |
    Published online on: December 15, 2011
       https://doi.org/10.3892/ijo.2011.1303
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Abstract

Osteosarcoma is the most common primary malignancy of the bone. There have been some advances in surgical and chemotherapeutic strategies, but it is still a tumor with a high mortality rate in children and young adults. Mitogen-activated protein kinase/extracellular signal regulated kinase (ERK) pathway plays an essential role in the development and progression of various tumors. ERK1/2 is a key component of this pathway and hyperactivated in different tumors including osteosarcoma. This study aimed to investigate whether downregulation of ERK1/2 by siRNA (small interfering RNA) could inhibit cell proliferation and invasion and increase chemosensitivity to cisplatin in human osteosarcoma U2-OS cells in vitro. Results showed that the downregulation of ERK1/2 expression by siRNA in human osteosarcoma cells significantly inhibited cell proliferation and invasion in vitro. Furthermore, ERK1/2 knockdown led to cell arrest in the G1/G0 phase of the cell cycle, and eventual apoptosis and chemosensitivity enhancement in tumor cells. Our data reveal that RNAi-mediated downregulation of ERK1/2 expression can lead to potent antitumor activity and chemosensitizing effects in human osteosarcoma.
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Copy and paste a formatted citation
Spandidos Publications style
Si H, Peng C, Li J, Wang X, Zhai L, Li X and Li J: RNAi-mediated knockdown of ERK1/2 inhibits cell proliferation and invasion and increases chemosensitivity to cisplatin in human osteosarcoma U2-OS cells in vitro. Int J Oncol 40: 1291-1297, 2012.
APA
Si, H., Peng, C., Li, J., Wang, X., Zhai, L., Li, X., & Li, J. (2012). RNAi-mediated knockdown of ERK1/2 inhibits cell proliferation and invasion and increases chemosensitivity to cisplatin in human osteosarcoma U2-OS cells in vitro. International Journal of Oncology, 40, 1291-1297. https://doi.org/10.3892/ijo.2011.1303
MLA
Si, H., Peng, C., Li, J., Wang, X., Zhai, L., Li, X., Li, J."RNAi-mediated knockdown of ERK1/2 inhibits cell proliferation and invasion and increases chemosensitivity to cisplatin in human osteosarcoma U2-OS cells in vitro". International Journal of Oncology 40.4 (2012): 1291-1297.
Chicago
Si, H., Peng, C., Li, J., Wang, X., Zhai, L., Li, X., Li, J."RNAi-mediated knockdown of ERK1/2 inhibits cell proliferation and invasion and increases chemosensitivity to cisplatin in human osteosarcoma U2-OS cells in vitro". International Journal of Oncology 40, no. 4 (2012): 1291-1297. https://doi.org/10.3892/ijo.2011.1303
Copy and paste a formatted citation
x
Spandidos Publications style
Si H, Peng C, Li J, Wang X, Zhai L, Li X and Li J: RNAi-mediated knockdown of ERK1/2 inhibits cell proliferation and invasion and increases chemosensitivity to cisplatin in human osteosarcoma U2-OS cells in vitro. Int J Oncol 40: 1291-1297, 2012.
APA
Si, H., Peng, C., Li, J., Wang, X., Zhai, L., Li, X., & Li, J. (2012). RNAi-mediated knockdown of ERK1/2 inhibits cell proliferation and invasion and increases chemosensitivity to cisplatin in human osteosarcoma U2-OS cells in vitro. International Journal of Oncology, 40, 1291-1297. https://doi.org/10.3892/ijo.2011.1303
MLA
Si, H., Peng, C., Li, J., Wang, X., Zhai, L., Li, X., Li, J."RNAi-mediated knockdown of ERK1/2 inhibits cell proliferation and invasion and increases chemosensitivity to cisplatin in human osteosarcoma U2-OS cells in vitro". International Journal of Oncology 40.4 (2012): 1291-1297.
Chicago
Si, H., Peng, C., Li, J., Wang, X., Zhai, L., Li, X., Li, J."RNAi-mediated knockdown of ERK1/2 inhibits cell proliferation and invasion and increases chemosensitivity to cisplatin in human osteosarcoma U2-OS cells in vitro". International Journal of Oncology 40, no. 4 (2012): 1291-1297. https://doi.org/10.3892/ijo.2011.1303
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