Spandidos Publications Logo
  • About
    • About Spandidos
    • Aims and Scopes
    • Abstracting and Indexing
    • Editorial Policies
    • Reprints and Permissions
    • Job Opportunities
    • Terms and Conditions
    • Contact
  • Journals
    • All Journals
    • Oncology Letters
      • Oncology Letters
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Oncology
      • International Journal of Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular and Clinical Oncology
      • Molecular and Clinical Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Experimental and Therapeutic Medicine
      • Experimental and Therapeutic Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Molecular Medicine
      • International Journal of Molecular Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Biomedical Reports
      • Biomedical Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Reports
      • Oncology Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular Medicine Reports
      • Molecular Medicine Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • World Academy of Sciences Journal
      • World Academy of Sciences Journal
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Functional Nutrition
      • International Journal of Functional Nutrition
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Epigenetics
      • International Journal of Epigenetics
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Medicine International
      • Medicine International
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
  • Articles
  • Information
    • Information for Authors
    • Information for Reviewers
    • Information for Librarians
    • Information for Advertisers
    • Conferences
  • Language Editing
Spandidos Publications Logo
  • About
    • About Spandidos
    • Aims and Scopes
    • Abstracting and Indexing
    • Editorial Policies
    • Reprints and Permissions
    • Job Opportunities
    • Terms and Conditions
    • Contact
  • Journals
    • All Journals
    • Biomedical Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Experimental and Therapeutic Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Epigenetics
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Functional Nutrition
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Molecular Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Medicine International
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular and Clinical Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular Medicine Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Letters
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • World Academy of Sciences Journal
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
  • Articles
  • Information
    • For Authors
    • For Reviewers
    • For Librarians
    • For Advertisers
    • Conferences
  • Language Editing
Login Register Submit
  • This site uses cookies
  • You can change your cookie settings at any time by following the instructions in our Cookie Policy. To find out more, you may read our Privacy Policy.

    I agree
Search articles by DOI, keyword, author or affiliation
Search
Advanced Search
presentation
International Journal of Oncology
Join Editorial Board Propose a Special Issue
Print ISSN: 1019-6439 Online ISSN: 1791-2423
Journal Cover
April 2012 Volume 40 Issue 4

Full Size Image

Cover Legend PDF

Sign up for eToc alerts
Recommend to Library

Journals

International Journal of Molecular Medicine

International Journal of Molecular Medicine

International Journal of Molecular Medicine is an international journal devoted to molecular mechanisms of human disease.

International Journal of Oncology

International Journal of Oncology

International Journal of Oncology is an international journal devoted to oncology research and cancer treatment.

Molecular Medicine Reports

Molecular Medicine Reports

Covers molecular medicine topics such as pharmacology, pathology, genetics, neuroscience, infectious diseases, molecular cardiology, and molecular surgery.

Oncology Reports

Oncology Reports

Oncology Reports is an international journal devoted to fundamental and applied research in Oncology.

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine is an international journal devoted to laboratory and clinical medicine.

Oncology Letters

Oncology Letters

Oncology Letters is an international journal devoted to Experimental and Clinical Oncology.

Biomedical Reports

Biomedical Reports

Explores a wide range of biological and medical fields, including pharmacology, genetics, microbiology, neuroscience, and molecular cardiology.

Molecular and Clinical Oncology

Molecular and Clinical Oncology

International journal addressing all aspects of oncology research, from tumorigenesis and oncogenes to chemotherapy and metastasis.

World Academy of Sciences Journal

World Academy of Sciences Journal

Multidisciplinary open-access journal spanning biochemistry, genetics, neuroscience, environmental health, and synthetic biology.

International Journal of Functional Nutrition

International Journal of Functional Nutrition

Open-access journal combining biochemistry, pharmacology, immunology, and genetics to advance health through functional nutrition.

International Journal of Epigenetics

International Journal of Epigenetics

Publishes open-access research on using epigenetics to advance understanding and treatment of human disease.

Medicine International

Medicine International

An International Open Access Journal Devoted to General Medicine.

Journal Cover
April 2012 Volume 40 Issue 4

Full Size Image

Cover Legend PDF

Sign up for eToc alerts
Recommend to Library

  • Article
  • Citations
    • Cite This Article
    • Download Citation
    • Create Citation Alert
    • Remove Citation Alert
    • Cited By
  • Similar Articles
    • Related Articles (in Spandidos Publications)
    • Similar Articles (Google Scholar)
    • Similar Articles (PubMed)
  • Download PDF
  • Download XML
  • View XML
Article

Possible association of decreased NKG2D expression levels and suppression of the activity of natural killer cells in patients with colorectal cancer

  • Authors:
    • Yajuan Shen
    • Chao Lu
    • Wenjun Tian
    • Laicheng Wang
    • Bin Cui
    • Yulian Jiao
    • Chunyan Ma
    • Ying Ju
    • Ling Zhu
    • Chunhong Shao
    • Xinqi Liu
    • Jian Wang
    • Bingchang Zhang
    • Zhiming Lu
  • View Affiliations / Copyright

    Affiliations: Department of Clinical Laboratory, Shandong Provincial Hospital Affiliated to Shandong University, Jinan 250021, P.R. China
  • Pages: 1285-1290
    |
    Published online on: December 22, 2011
       https://doi.org/10.3892/ijo.2011.1315
  • Expand metrics +
Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )
Metrics: Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )
Cited By (CrossRef): 0 citations Loading Articles...

This article is mentioned in:



Abstract

Natural-killer group 2 (NKG2), a natural killer (NK) cell receptor, plays a critical role in regulating NK cytotoxicity. In this study, we investigated the expression levels of natural killer group 2 member A (NKG2A) and natural killer group 2 member D (NKG2D) in NK cells as well as the regulatory function of NKG2D in patients with colorectal cancer (CRC). Sixty-two CRC patients and 32 healthy controls were enrolled in this study. The expression levels of NKG2A and NKG2D mRNA in peripheral blood mononuclear cells (PBMCs) were investigated using real-time PCR. Flow cytometry was performed to assay the levels of NKG2A and NKG2D proteins in NK cells. The levels of NKG2D mRNA in PBMCs in the patients were significantly lower than those in the controls [mean ± SD, 1.11±0.60 (CRC patients) vs. 1.65±0.71 (healthy controls); p<0.01], whereas the 2 groups showed no apparent difference in the levels of NKG2A mRNA (p>0.05). In addition, the patients showed significantly lower NKG2D levels in NK cells than the controls did (71.23%±8.31% [CRC patients] vs. 79.39%±5.58% [healthy controls]; p<0.01). However, we observed no distinct difference in the NKG2A expression levels in NK cells between the 2 groups (p>0.05). Notably, blockage of NKG2D signaling with anti-NKG2D antibodies ex vivo resulted in decreased cytotoxicity and CD107a degranulation. Our data revealed that the decrease in NKG2D expression levels may have been associated with suppression of NK cell activity in CRC patients.
View Figures

Figure 1

Figure 2

Figure 3

Figure 4

Figure 5

View References

1 

Jemal A, Siegel R, Ward E, et al: Cancer statistics, 2008. CA Cancer J Clin. 58:71–96. 2008. View Article : Google Scholar

2 

Jemal A, Siegel R, Ward E, Hao Y, Xu J and Thun MJ: Cancer statistics, 2009. CA Cancer J Clin. 59:225–249. 2009. View Article : Google Scholar

3 

Jemal A, Siegel R, Xu J and Ward E: Cancer statistics, 2010. CA Cancer J Clin. 60:277–300. 2010. View Article : Google Scholar

4 

Jemal A, Bray F, Center MM, Ferlay J, Ward E and Forman D: Global cancer statistics. CA Cancer J Clin. 61:69–90. 2011. View Article : Google Scholar

5 

Lanier LL: NK cell receptors. Annu Rev Immunol. 16:359–393. 1998. View Article : Google Scholar

6 

Bauer S, Groh V, Wu J, et al: Activation of NK cells and T cells by NKG2D, a receptor for stress-inducible MICA. Science. 285:727–729. 1999. View Article : Google Scholar : PubMed/NCBI

7 

Eleme K, Taner SB, Onfelt B, et al: Cell surface organization of stress-inducible proteins ULBP and MICA that stimulate human NK cells and T cells via NKG2D. J Exp Med. 199:1005–1010. 2004. View Article : Google Scholar : PubMed/NCBI

8 

Ota T, Takeda K, Akiba H, et al: IFN-gamma-mediated negative feedback regulation of NKT-cell function by CD94/NKG2. Blood. 106:184–192. 2005. View Article : Google Scholar : PubMed/NCBI

9 

Sheu BC, Chiou SH, Lin HH, et al: Up-regulation of inhibitory natural killer receptors CD94/NKG2A with suppressed intracellular perforin expression of tumor-infiltrating CD8+ T lymphocytes in human cervical carcinoma. Cancer Res. 65:2921–2929. 2005. View Article : Google Scholar : PubMed/NCBI

10 

Lanier LL: Follow the leader: NK cell receptors for classical and nonclassical MHC class I. Cell. 92:705–707. 1998. View Article : Google Scholar : PubMed/NCBI

11 

Groh V, Rhinehart R, Secrist H, Bauer S, Grabstein KH and Spies T: Broad tumor-associated expression and recognition by tumor-derived gamma delta T cells of MICA and MICB. Proc Natl Acad Sci USA. 96:6879–6884. 1999. View Article : Google Scholar

12 

Wu J, Song Y, Bakker AB, et al: An activating immunoreceptor complex formed by NKG2D and DAP10. Science. 285:730–732. 1999. View Article : Google Scholar : PubMed/NCBI

13 

Yamamoto K, Fujiyama Y, Andoh A, Bamba T and Okabe H: Oxidative stress increases MICA and MICB gene expression in the human colon carcinoma cell line (CaCo-2). Biochim Biophys Acta. 1526:10–12. 2001. View Article : Google Scholar : PubMed/NCBI

14 

Gasser S, Orsulic S, Brown EJ and Raulet DH: The DNA damage pathway regulates innate immune system ligands of the NKG2D receptor. Nature. 436:1186–1190. 2005. View Article : Google Scholar : PubMed/NCBI

15 

Palmisano GL, Contardi E, Morabito A, Gargaglione V, Ferrara GB and Pistillo MP: HLA-E surface expression is independent of the availability of HLA class I signal sequence-derived peptides in human tumor cell lines. Hum Immunol. 66:1–12. 2005. View Article : Google Scholar : PubMed/NCBI

16 

Cao W, Xi X, Wang Z, et al: Four novel ULBP splice variants are ligands for human NKG2D. Int Immunol. 20:981–991. 2008. View Article : Google Scholar : PubMed/NCBI

17 

Guerra N, Tan YX, Joncker NT, et al: NKG2D-deficient mice are defective in tumor surveillance in models of spontaneous malignancy. Immunity. 28:571–580. 2008. View Article : Google Scholar : PubMed/NCBI

18 

Ljunggren HG: Cancer immunosurveillance: NKG2D breaks cover. Immunity. 28:492–494. 2008. View Article : Google Scholar : PubMed/NCBI

19 

Melum E, Buch S, Schafmayer C, et al: Investigation of cholangiocarcinoma associated NKG2D polymorphisms in colorectal carcinoma. Int J Cancer. 123:241–242. 2008. View Article : Google Scholar : PubMed/NCBI

20 

Nausch N and Cerwenka A: NKG2D ligands in tumor immunity. Oncogene. 27:5944–5958. 2008. View Article : Google Scholar : PubMed/NCBI

21 

Barber A and Sentman CL: Chimeric NKG2D T cells require both T cell-and host-derived cytokine secretion and perforin expression to increase tumor antigen presentation and systemic immunity. J Immunol. 183:2365–2372. 2009. View Article : Google Scholar : PubMed/NCBI

22 

Berezhnoi AE, Chernisheva AD, Zakeeva IR, et al: HLA-E molecule induction on the surface of tumor cells protects them from cytotoxic lymphocytes. Vopr Onkol. 55:224–229. 2009.(In Russian).

23 

Kopp R, Glas J, Lau-Werner U, Albert ED and Weiss EH: Association of MICA-TM and MICB C1_2_A microsatellite polymorphisms with tumor progression in patients with colorectal cancer. J Clin Immunol. 29:545–554. 2009. View Article : Google Scholar : PubMed/NCBI

24 

Zeng XH, Sha WH, Li YY, Nie YQ, Li QN and Liang PZ: Expression of NK cells receptor NKG2D from peripheral blood in patients with primary hepatic carcinoma and its clinical significance. Zhonghua Yi Xue Za Zhi. 89:1272–1274. 2009.(In Chinese).

25 

Bjorklund AT, Schaffer M, Fauriat C, et al: NK cells expressing inhibitory KIR for non-self-ligands remain tolerant in HLA-matched sibling stem cell transplantation. Blood. 115:2686–2694. 2010. View Article : Google Scholar : PubMed/NCBI

26 

Kren L, Slaby O, Muckova K, et al: Expression of immune-modulatory molecules HLA-G and HLA-E by tumor cells in glioblastomas: an unexpected prognostic significance? Neuropathology. 31:129–134. 2011. View Article : Google Scholar : PubMed/NCBI

27 

Zhang C, Zhang J, Sun R, Feng J, Wei H and Tian Z: Opposing effect of IFNgamma and IFNalpha on expression of NKG2 receptors: negative regulation of IFNgamma on NK cells. Int Immunopharmacol. 5:1057–1067. 2005. View Article : Google Scholar : PubMed/NCBI

28 

Zhang C, Zhang J, Niu J, Zhou Z and Tian Z: Interleukin-12 improves cytotoxicity of natural killer cells via upregulated expression of NKG2D. Hum Immunol. 69:490–500. 2008. View Article : Google Scholar : PubMed/NCBI

29 

Serrano AE, Menares-Castillo E, Garrido-Tapia M, et al: Interleukin 10 decreases MICA expression on melanoma cell surface. Immunol Cell Biol. 89:447–457. 2011. View Article : Google Scholar : PubMed/NCBI

30 

Zhang J, Sun R, Wei H and Tian Z: Characterization of interleukin-15 gene-modified human natural killer cells: implications for adoptive cellular immunotherapy. Haematologica. 89:338–347. 2004.PubMed/NCBI

Related Articles

  • Abstract
  • View
  • Download
  • Twitter
Copy and paste a formatted citation
Spandidos Publications style
Shen Y, Lu C, Tian W, Wang L, Cui B, Jiao Y, Ma C, Ju Y, Zhu L, Shao C, Shao C, et al: Possible association of decreased NKG2D expression levels and suppression of the activity of natural killer cells in patients with colorectal cancer. Int J Oncol 40: 1285-1290, 2012.
APA
Shen, Y., Lu, C., Tian, W., Wang, L., Cui, B., Jiao, Y. ... Lu, Z. (2012). Possible association of decreased NKG2D expression levels and suppression of the activity of natural killer cells in patients with colorectal cancer. International Journal of Oncology, 40, 1285-1290. https://doi.org/10.3892/ijo.2011.1315
MLA
Shen, Y., Lu, C., Tian, W., Wang, L., Cui, B., Jiao, Y., Ma, C., Ju, Y., Zhu, L., Shao, C., Liu, X., Wang, J., Zhang, B., Lu, Z."Possible association of decreased NKG2D expression levels and suppression of the activity of natural killer cells in patients with colorectal cancer". International Journal of Oncology 40.4 (2012): 1285-1290.
Chicago
Shen, Y., Lu, C., Tian, W., Wang, L., Cui, B., Jiao, Y., Ma, C., Ju, Y., Zhu, L., Shao, C., Liu, X., Wang, J., Zhang, B., Lu, Z."Possible association of decreased NKG2D expression levels and suppression of the activity of natural killer cells in patients with colorectal cancer". International Journal of Oncology 40, no. 4 (2012): 1285-1290. https://doi.org/10.3892/ijo.2011.1315
Copy and paste a formatted citation
x
Spandidos Publications style
Shen Y, Lu C, Tian W, Wang L, Cui B, Jiao Y, Ma C, Ju Y, Zhu L, Shao C, Shao C, et al: Possible association of decreased NKG2D expression levels and suppression of the activity of natural killer cells in patients with colorectal cancer. Int J Oncol 40: 1285-1290, 2012.
APA
Shen, Y., Lu, C., Tian, W., Wang, L., Cui, B., Jiao, Y. ... Lu, Z. (2012). Possible association of decreased NKG2D expression levels and suppression of the activity of natural killer cells in patients with colorectal cancer. International Journal of Oncology, 40, 1285-1290. https://doi.org/10.3892/ijo.2011.1315
MLA
Shen, Y., Lu, C., Tian, W., Wang, L., Cui, B., Jiao, Y., Ma, C., Ju, Y., Zhu, L., Shao, C., Liu, X., Wang, J., Zhang, B., Lu, Z."Possible association of decreased NKG2D expression levels and suppression of the activity of natural killer cells in patients with colorectal cancer". International Journal of Oncology 40.4 (2012): 1285-1290.
Chicago
Shen, Y., Lu, C., Tian, W., Wang, L., Cui, B., Jiao, Y., Ma, C., Ju, Y., Zhu, L., Shao, C., Liu, X., Wang, J., Zhang, B., Lu, Z."Possible association of decreased NKG2D expression levels and suppression of the activity of natural killer cells in patients with colorectal cancer". International Journal of Oncology 40, no. 4 (2012): 1285-1290. https://doi.org/10.3892/ijo.2011.1315
Follow us
  • Twitter
  • LinkedIn
  • Facebook
About
  • Spandidos Publications
  • Careers
  • Cookie Policy
  • Privacy Policy
How can we help?
  • Help
  • Live Chat
  • Contact
  • Email to our Support Team