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International Journal of Molecular Medicine

International Journal of Molecular Medicine

International Journal of Molecular Medicine is an international journal devoted to molecular mechanisms of human disease.

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International Journal of Oncology

International Journal of Oncology is an international journal devoted to oncology research and cancer treatment.

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Covers molecular medicine topics such as pharmacology, pathology, genetics, neuroscience, infectious diseases, molecular cardiology, and molecular surgery.

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Oncology Reports is an international journal devoted to fundamental and applied research in Oncology.

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Experimental and Therapeutic Medicine is an international journal devoted to laboratory and clinical medicine.

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Oncology Letters is an international journal devoted to Experimental and Clinical Oncology.

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Molecular and Clinical Oncology

International journal addressing all aspects of oncology research, from tumorigenesis and oncogenes to chemotherapy and metastasis.

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Multidisciplinary open-access journal spanning biochemistry, genetics, neuroscience, environmental health, and synthetic biology.

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Article

MicroRNA-200a suppresses the Wnt/β-catenin signaling pathway by interacting with β-catenin

  • Authors:
    • Juan Su
    • Anling Zhang
    • Zhendong Shi
    • Feifei Ma
    • Peiyu Pu
    • Tao Wang
    • Jie Zhang
    • Chunsheng Kang
    • Qingyu Zhang
  • View Affiliations / Copyright

    Affiliations: Department of Gastroenterology, Tianjin Medical University General Hospital, Heping District, Tianjin 300052, P.R. China, Department of Neurosurgery, Tianjin Medical University General Hospital and Laboratory of Neuro-Oncology, Tianjin Neurological Institute, Heping District, Tianjin 300052, P.R. China, Tianjin Medical College, Tianjin 300011, P.R. China
  • Pages: 1162-1170
    |
    Published online on: December 30, 2011
       https://doi.org/10.3892/ijo.2011.1322
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Abstract

The Wnt/β-catenin signaling pathway is crucial for human organ development and is involved in tumor progression of many cancers. Accumulating evidence suggests that the expression of β-catenin is, in part, regulated by specific microRNAs (miRNAs). The purpose of this study was to determine the expression of a recently identified epithelial to mesenchymal transition (EMT)-associated tumor suppressor microRNA (miR)-200a, in cancer cells. We also aimed to identify specific miR-200a target genes and to investigate the antitumor effects of miR-200a on the Wnt/β-catenin signaling pathway. We employed TOP/FOP flash luciferase assays to identify the effect of miR-200a on the Wnt/β-catenin pathway and we confirmed our observations using fluorescence microscopy. To determine target genes of miR-200a, a 3' untranslated region (3' UTR) luciferase assay was performed. Cell viability, invasion and wound healing assays were carried out for functional analysis after miRNA transfection. We further investigated the role of miR-200a in EMT by Western blot analysis. We found fluctuation in the expression of miR-200a that was accompanied by changes in the expression of members of the Wnt/β-catenin signaling pathway. We also determined that miR-200a can directly interact with the 3' UTR of CTNNB1 (the gene that encodes β-catenin) to suppress Wnt/β-catenin signaling. MiR-200a could also influence the biological activities of SGC790 and U251 cells. Our results demonstrate that miR-200a is a new tumor suppressor that can regulate the activity of the Wnt/β-catenin signaling pathway via two mechanisms. MiR-200a is a candidate target for tumor treatment via its regulation of the Wnt/β-catenin signaling pathway.
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Copy and paste a formatted citation
Spandidos Publications style
Su J, Zhang A, Shi Z, Ma F, Pu P, Wang T, Zhang J, Kang C and Zhang Q: MicroRNA-200a suppresses the Wnt/β-catenin signaling pathway by interacting with β-catenin. Int J Oncol 40: 1162-1170, 2012.
APA
Su, J., Zhang, A., Shi, Z., Ma, F., Pu, P., Wang, T. ... Zhang, Q. (2012). MicroRNA-200a suppresses the Wnt/β-catenin signaling pathway by interacting with β-catenin. International Journal of Oncology, 40, 1162-1170. https://doi.org/10.3892/ijo.2011.1322
MLA
Su, J., Zhang, A., Shi, Z., Ma, F., Pu, P., Wang, T., Zhang, J., Kang, C., Zhang, Q."MicroRNA-200a suppresses the Wnt/β-catenin signaling pathway by interacting with β-catenin". International Journal of Oncology 40.4 (2012): 1162-1170.
Chicago
Su, J., Zhang, A., Shi, Z., Ma, F., Pu, P., Wang, T., Zhang, J., Kang, C., Zhang, Q."MicroRNA-200a suppresses the Wnt/β-catenin signaling pathway by interacting with β-catenin". International Journal of Oncology 40, no. 4 (2012): 1162-1170. https://doi.org/10.3892/ijo.2011.1322
Copy and paste a formatted citation
x
Spandidos Publications style
Su J, Zhang A, Shi Z, Ma F, Pu P, Wang T, Zhang J, Kang C and Zhang Q: MicroRNA-200a suppresses the Wnt/β-catenin signaling pathway by interacting with β-catenin. Int J Oncol 40: 1162-1170, 2012.
APA
Su, J., Zhang, A., Shi, Z., Ma, F., Pu, P., Wang, T. ... Zhang, Q. (2012). MicroRNA-200a suppresses the Wnt/β-catenin signaling pathway by interacting with β-catenin. International Journal of Oncology, 40, 1162-1170. https://doi.org/10.3892/ijo.2011.1322
MLA
Su, J., Zhang, A., Shi, Z., Ma, F., Pu, P., Wang, T., Zhang, J., Kang, C., Zhang, Q."MicroRNA-200a suppresses the Wnt/β-catenin signaling pathway by interacting with β-catenin". International Journal of Oncology 40.4 (2012): 1162-1170.
Chicago
Su, J., Zhang, A., Shi, Z., Ma, F., Pu, P., Wang, T., Zhang, J., Kang, C., Zhang, Q."MicroRNA-200a suppresses the Wnt/β-catenin signaling pathway by interacting with β-catenin". International Journal of Oncology 40, no. 4 (2012): 1162-1170. https://doi.org/10.3892/ijo.2011.1322
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