Inhibition of cell viability by human IFN-β is mediated by microRNA-431

Tanaka,Takeshi; Sugaya,Shigeru; Kita,Kazuko; Arai,Makoto; Kanda,Tatsuo; Fujii,Katsunori; Imazeki,Fumio; Sugita,Katsuo; Yokosuka,Osamu; Suzuki,Nobuo

doi:10.3892/ijo.2012.1345

May 2012 Volume 40 Issue 5

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May 2012 Volume 40 Issue 5

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Article

Inhibition of cell viability by human IFN-β is mediated by microRNA-431

Authors:
- Takeshi Tanaka
- Shigeru Sugaya
- Kazuko Kita
- Makoto Arai
- Tatsuo Kanda
- Katsunori Fujii
- Fumio Imazeki
- Katsuo Sugita
- Osamu Yokosuka
- Nobuo Suzuki
View Affiliations / Copyright

Affiliations: Department of Environmental Biochemistry, Graduate School of Medicine, Chiba University, 1-8-1 Inohana, Chiba 260-8670, Japan, Department of Medicine and Clinical Oncology, Graduate School of Medicine, Chiba University, 1-8-1 Inohana, Chiba 260-8670, Japan, Department of Pediatrics, Graduate School of Medicine, Chiba University, 1-8-1 Inohana, Chiba 260-8670, Japan, Faculty of Education, Chiba University, 1-33 Yayoichou, Chiba 263-8522, Japan
Pages: 1470-1476
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Published online on: January 24, 2012

https://doi.org/10.3892/ijo.2012.1345
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Abstract

MicroRNAs (miRNAs) are small non-coding RNAs that inhibit gene expression by cleaving or hindering the translation of target mRNAs. We used microarray-based comparative transcriptome analysis to identify changes in miRNA expression and function between a human cell line, RSa, which is highly sensitive to HuIFN-β-mediated inhibition of cell viability, and its variant, the F-IFr cell line, which is relatively resistant to the cytokine. miR-431 expression was significantly higher in RSa cells compared with F-IFr cells. The addition of HuIFN-β to RSa cultures reduced cell viability, down-regulated expression of IGF1R and IRS2 (putative miR-431 target genes), and inhibited the PI3K-Akt and MAPK pathways. The survival of F-IFr cells was not reduced by HuIFN-β, but transient transfection with miR-431 precursors significantly decreased viability and concomitantly down-regulated IGF1R and IRS2 expression. In addition, the MAPK pathway, but not the PI3K-Akt pathway, was suppressed in F-IFr cells. Based on these results, we propose that, in RSa cells, HuIFN-β-induced miR-431 expression may down-regulate IGF1R and IRS2 expression, and consequently inhibit cell proliferation by suppressing the MAPK pathway.

Journals

International Journal of Molecular Medicine

International Journal of Oncology

Molecular Medicine Reports

Oncology Reports

Experimental and Therapeutic Medicine

Oncology Letters

Biomedical Reports

Molecular and Clinical Oncology

World Academy of Sciences Journal

International Journal of Functional Nutrition

International Journal of Epigenetics

Medicine International

Inhibition of cell viability by human IFN-β is mediated by microRNA-431

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